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Description:
Probable endonuclease IV (EC 3.1.21.2) (Endodeoxyribonuclease IV).
Molecular weight: 31685
View which proteins in this organism that is involved with DNA Repair;
classified after biological processes (using data from the GOA project):
DNA repair( GO:0006281 )
Important dates:
28-NOV-2002, integrated into UniProtKB/Swiss-Prot.
01-OCT-2001, sequence version 1.
07-MAR-2006, entry version 27.
Phylogenetic order:
Bacteria Firmicutes Mollicutes Mycoplasmataceae Mycoplasma.
To calculate the pI (Isoelectric point - the pH where a protein has a neutral charge),
go to this page and enter the protein ID (e.g 3MG_ECOLI): http://us.expasy.org/tools/pi_tool.html
Links to references in other databases for protein END4_MYCPU:
| Database | Pointer | Add. info#1 | Add. info#2 |
| EMBL | AL445565 | CAC13794.1 | - |
| PIR | E90589 | E90589. | |
| HSSP | P12638 | 1QTW | |
| GenomeReviews | AL445566_GR | MYPU_6210.1 | |
| MypuList | MYPU_6210 | -. | |
| BioCyc | MPUL2107:MYPU_6210-MONOMER | -.1 | |
| HAMAP | MF_00152 | - | 1. |
| InterPro | IPR001719 | AP_endnuclease2. | |
| InterPro | IPR012307 | Xylisom_TIMbarrl. | |
| Pfam | PF01261 | AP_endonuc_2 | 1. |
| SMART | SM00518 | AP2Ec | 1. |
| TIGRFAMs | TIGR00587 | nfo | 1. |
| PROSITE | PS00729 | AP_NUCLEASE_F2_1 | 1. |
| PROSITE | PS00730 | AP_NUCLEASE_F2_2 | FALSE_NEG. |
| PROSITE | PS00731 | AP_NUCLEASE_F2_3 | 1. |
Keywords:
Complete proteome; DNA damage; DNA repair; Endonuclease; Hydrolase; Metal-binding; Nuclease; Zinc.
References:
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=UAB CTIP;
RX MEDLINE=21267165; PubMed=11353084; DOI=10.1093/nar/29.10.2145;
RA Chambaud I., Heilig R., Ferris S., Barbe V., Samson D., Galisson F.,
RA Moszer I., Dybvig K., Wroblewski H., Viari A., Rocha E.P.C.,
RA Blanchard A.;
RT "The complete genome sequence of the murine respiratory pathogen
RT Mycoplasma pulmonis.";
RL Nucleic Acids Res. 29:2145-2153(2001).
Feature:
CHAIN 1 277 Probable endonuclease IV.
/FTId=PRO_0000190859.
METAL 70 70 Zinc 1 (By similarity).
METAL 108 108 Zinc 1 (By similarity).
METAL 145 145 Zinc 1 (By similarity).
METAL 145 145 Zinc 2 (By similarity).
METAL 178 178 Zinc 2 (By similarity).
METAL 181 181 Zinc 3 (By similarity).
METAL 212 212 Zinc 2 (By similarity).
METAL 225 225 Zinc 3 (By similarity).
METAL 227 227 Zinc 3 (By similarity).
METAL 257 257 Zinc 2 (By similarity).
Comments:
-!- FUNCTION: Endonuclease IV plays a role in DNA repair. It cleaves
phosphodiester bonds at apurinic or apyrimidinic sites (AP sites)
to produce new 5' ends that are base-free deoxyribose 5-phosphate
residues. It preferentially attacks modified AP sites created by
bleomycin and neocarzinostatin (By similarity).
-!- CATALYTIC ACTIVITY: Endonucleolytic cleavage to 5'-
phosphooligonucleotide end-products.
-!- COFACTOR: Binds 3 zinc ions (By similarity).
-!- SIMILARITY: Belongs to the AP endonuclease 2 family.
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Sequence length: 277
MIKLGSHVSF KSPNYLYDSI KESIKNGANC AMIFLGAPQN TKRVDFEKYQ YEKYLKDFSN
LIKPEDIVVH APYIINPASL EKADFAISFL SSEIKRMDKA KFKYLVLHPG FYGKNNVKDS
LDQLARSIQK IFEITKDSNV EIMLETMSGK GSEVGKSYEE ILYVIDKVKS PRLGACLDTC
HVWDAGYNIN DYQVFKDELI KTGILKHIKV IHLNDSKNEL GSHKDRHANI DKGLIGLKNL
KRIVHDPIFE NIPIILETPW TEKGPIYDQE IAMLLEK