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Description:
LexA repressor (EC 3.4.21.88).
Molecular weight: 26049
View which proteins in this organism that is involved with DNA Repair;
classified after biological processes (using data from the GOA project):
DNA repair( GO:0006281 )
Important dates:
28-MAR-2003, integrated into UniProtKB/Swiss-Prot.
01-MAR-2003, sequence version 1.
07-MAR-2006, entry version 23.
Phylogenetic order:
Bacteria Actinobacteria Actinobacteridae Bifidobacteriales Bifidobacteriaceae Bifidobacterium.
To calculate the pI (Isoelectric point - the pH where a protein has a neutral charge),
go to this page and enter the protein ID (e.g 3MG_ECOLI): http://us.expasy.org/tools/pi_tool.html
Links to references in other databases for protein LEXA_BIFLO:
| Database | Pointer | Add. info#1 | Add. info#2 |
| EMBL | AE014295 | AAN25110.1 | - |
| HSSP | P03033 | 1JHC | |
| GenomeReviews | AE014295_GR | BL1310.1 | |
| BioCyc | BLON206672:BL1310-MONOMER | -.1 | |
| HAMAP | MF_00015 | - | 1. |
| InterPro | IPR006199 | LexA_DNA_bd. | |
| InterPro | IPR006200 | Pept_S24_LexA. | |
| InterPro | IPR006198 | Pept_S24_S26. | |
| InterPro | IPR006197 | Pept_S24_SOS. | |
| InterPro | IPR013324 | Sigma_r3_r4. | |
| InterPro | IPR011991 | Wing_hlx_DNA_bd. | |
| Pfam | PF01726 | LexA_DNA_bind | 1. |
| Pfam | PF00717 | Peptidase_S24 | 1. |
| PRINTS | PR00726 | LEXASERPTASE. | |
| TIGRFAMs | TIGR00498 | lexA | 1. |
Keywords:
Autocatalytic cleavage; Complete proteome; DNA damage; DNA repair; DNA replication; DNA-binding; Hydrolase; Repressor; SOS response; Transcription; Transcription regulation.
References:
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=NCC 2705;
RX MEDLINE=22294977; PubMed=12381787; DOI=10.1073/pnas.212527599;
RA Schell M.A., Karmirantzou M., Snel B., Vilanova D., Berger B.,
RA Pessi G., Zwahlen M.-C., Desiere F., Bork P., Delley M.,
RA Pridmore R.D., Arigoni F.;
RT "The genome sequence of Bifidobacterium longum reflects its adaptation
RT to the human gastrointestinal tract.";
RL Proc. Natl. Acad. Sci. U.S.A. 99:14422-14427(2002).
Feature:
CHAIN 1 241 LexA repressor.
/FTId=PRO_0000170013.
DNA_BIND 41 61 H-T-H motif (By similarity).
ACT_SITE 165 165 Involved in auto-cleavage (By
similarity).
ACT_SITE 202 202 Involved in auto-cleavage (By
similarity).
SITE 130 131 Cleavage (auto-) (By similarity).
Comments:
-!- FUNCTION: Represses a number of genes involved in the response to
DNA damage (SOS response), including recA and lexA. In the
presence of single-stranded DNA, recA interacts with lexA causing
an autocatalytic cleavage which disrupts the DNA-binding part of
lexA, leading to derepression of the SOS regulon and eventually
DNA repair (By similarity).
-!- CATALYTIC ACTIVITY: Hydrolysis of Ala-|-Gly bond in repressor
lexA.
-!- SUBUNIT: Homodimer (By similarity).
-!- SIMILARITY: Belongs to the peptidase S24 family.
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Sequence length: 241
MSTIPFSPKQ KPDESTLTDR QRKVLDAIRT HIDEQGFAPS FREIGNAAGL KSPSSVKHQL
QVLEDKGFIR MNANKGRAIE VVAGSAPNAE KPSQASEEAT STSNVAEIYQ FPAEAIAESH
DVPLVGRIAA GVPITAEQHV DDVMRLPERL TGSGTLFMLE VHGDSMVDAA ICDGDYVVVR
EQNSAVNGDI VAALLDDEAT VKTFRKENGH VWLMPHNPAY SPIDGTHATI MGKVVTVLRK
L