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Description:
Flap structure-specific endonuclease (EC 3.1.-.-).
Molecular weight: 37527
View which proteins in this organism that is involved with DNA Repair;
classified after biological processes (using data from the GOA project):
DNA repair( GO:0006281 )
Important dates:
05-DEC-2001, integrated into UniProtKB/Swiss-Prot.
01-NOV-1996, sequence version 1.
07-MAR-2006, entry version 41.
Phylogenetic order:
Archaea Euryarchaeota Methanococci Methanococcales Methanocaldococcaceae Methanocaldococcus.
To calculate the pI (Isoelectric point - the pH where a protein has a neutral charge),
go to this page and enter the protein ID (e.g 3MG_ECOLI): http://us.expasy.org/tools/pi_tool.html
Links to references in other databases for protein FEN_METJA:
| Database | Pointer | Add. info#1 | Add. info#2 |
| EMBL | L77117 | AAB99454.1 | - |
| PIR | C64480 | C64480. | |
| PDB | 1A76 | X-ray | @=1-326. |
| PDB | 1A77 | X-ray | @=1-326. |
| GenomeReviews | L77117_GR | MJ1444.1 | |
| TIGR | MJ1444 | -. | |
| BioCyc | MJAN243232:MJ1444-MONOMER | -.1 | |
| HAMAP | MF_00614 | - | 1. |
| InterPro | IPR000513 | Exo_N_I. | |
| InterPro | IPR008918 | HhH2. | |
| InterPro | IPR006086 | XPG_I. | |
| InterPro | IPR006085 | XPG_N. | |
| InterPro | IPR006084 | XPGC_Rad. | |
| Pfam | PF00867 | XPG_I | 1. |
| Pfam | PF00752 | XPG_N | 1. |
| PRINTS | PR00853 | XPGRADSUPER. | |
| SMART | SM00279 | HhH2 | 1. |
| SMART | SM00484 | XPGI | 1. |
| SMART | SM00485 | XPGN | 1. |
Keywords:
3D-structure; Complete proteome; Endonuclease; Hydrolase; Magnesium; Metal-binding; Nuclease.
References:
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=JAL-1 / DSM 2661 / ATCC 43067;
RX MEDLINE=96337999; PubMed=8688087;
RA Bult C.J., White O., Olsen G.J., Zhou L., Fleischmann R.D.,
RA Sutton G.G., Blake J.A., FitzGerald L.M., Clayton R.A., Gocayne J.D.,
RA Kerlavage A.R., Dougherty B.A., Tomb J.-F., Adams M.D., Reich C.I.,
RA Overbeek R., Kirkness E.F., Weinstock K.G., Merrick J.M., Glodek A.,
RA Scott J.L., Geoghagen N.S.M., Weidman J.F., Fuhrmann J.L., Nguyen D.,
RA Utterback T.R., Kelley J.M., Peterson J.D., Sadow P.W., Hanna M.C.,
RA Cotton M.D., Roberts K.M., Hurst M.A., Kaine B.P., Borodovsky M.,
RA Klenk H.-P., Fraser C.M., Smith H.O., Woese C.R., Venter J.C.;
RT "Complete genome sequence of the methanogenic archaeon, Methanococcus
RT jannaschii.";
RL Science 273:1058-1073(1996).
RN [2]
RP CHARACTERIZATION.
RX MEDLINE=98440432; PubMed=9765572;
RA Rao H.G., Rosenfeld A., Wetmur J.G.;
RT "Methanococcus jannaschii flap endonuclease: expression, purification,
RT and substrate requirements.";
RL J. Bacteriol. 180:5406-5412(1998).
RN [3]
RP CHARACTERIZATION, AND X-RAY CRYSTALLOGRAPHY (2.0 ANGSTROMS).
RX MEDLINE=99200693; PubMed=10102570;
RA Bae K.W., Baek K.W., Cho C.S., Hwang K.Y., Kim H.-R., Sung H.-C.,
RA Cho Y.;
RT "Expression, purification, characterization and crystallization of
RT flap endonuclease-1 from Methanococcus jannaschii.";
RL Mol. Cells 9:45-48(1999).
RN [4]
RP X-RAY CRYSTALLOGRAPHY (2.0 ANGSTROMS).
RC STRAIN=JAL-1 / DSM 2661 / ATCC 43067;
RX MEDLINE=98363215; PubMed=9699635;
RA Hwang K.Y., Baek K., Kim H.-Y., Cho Y.;
RT "The crystal structure of flap endonuclease-1 from Methanococcus
RT jannaschii.";
RL Nat. Struct. Biol. 5:707-713(1998).
Feature:
CHAIN 1 326 Flap structure-specific endonuclease.
/FTId=PRO_0000154053.
METAL 154 154 Magnesium 1.
HELIX 6 8
STRAND 9 9
STRAND 12 13
STRAND 16 16
HELIX 17 19
TURN 20 21
STRAND 23 27
HELIX 28 38
STRAND 39 40
TURN 42 43
STRAND 44 44
STRAND 46 46
STRAND 48 48
TURN 50 51
STRAND 52 52
STRAND 54 54
HELIX 56 70
TURN 71 72
STRAND 74 79
STRAND 83 84
STRAND 89 90
STRAND 93 94
STRAND 96 98
STRAND 100 101
STRAND 103 103
STRAND 110 110
HELIX 111 115
TURN 116 117
STRAND 118 118
TURN 119 120
HELIX 121 124
STRAND 126 127
HELIX 129 142
TURN 143 143
STRAND 146 148
STRAND 150 151
HELIX 153 162
TURN 163 164
STRAND 165 170
STRAND 172 174
HELIX 175 179
TURN 180 180
STRAND 182 190
STRAND 192 193
STRAND 196 199
HELIX 200 207
TURN 208 208
HELIX 211 221
TURN 224 225
STRAND 226 226
TURN 227 232
HELIX 235 243
TURN 244 245
HELIX 247 254
STRAND 255 255
TURN 256 257
HELIX 258 266
STRAND 267 267
HELIX 283 290
TURN 291 294
HELIX 298 315
Comments:
-!- FUNCTION: Endonuclease that cleave the 5'overhanging flap
structure that is generated by displacement synthesis when DNA
polymerase encounters the 5'end of a downstream Okazaki fragment.
Has 5'endo-/exonuclease and 5'pseudo-Y-endonuclease activities.
Cleaves the junction between single and double-stranded regions of
flap DNA.
-!- COFACTOR: Binds 2 magnesium ions per subunit.
-!- BIOPHYSICOCHEMICAL PROPERTIES:
pH dependence:
Optimum pH is 6-7;
-!- SIMILARITY: Belongs to the XPG/RAD2 endonuclease family. FEN1
subfamily.
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Sequence length: 326
MGVQFGDFIP KNIISFEDLK GKKVAIDGMN ALYQFLTSIR LRDGSPLRNR KGEITSAYNG
VFYKTIHLLE NDITPIWVFD GEPPKLKEKT RKVRREMKEK AELKMKEAIK KEDFEEAAKY
AKRVSYLTPK MVENCKYLLS LMGIPYVEAP SEGEAQASYM AKKGDVWAVV SQDYDALLYG
APRVVRNLTT TKEMPELIEL NEVLEDLRIS LDDLIDIAIF MGTDYNPGGV KGIGFKRAYE
LVRSGVAKDV LKKEVEYYDE IKRIFKEPKV TDNYSLSLKL PDKEGIIKFL VDENDFNYDR
VKKHVDKLYN LIANKTKQKT LDAWFK