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Description:
N-glycosylase/DNA lyase [Includes: 8-oxoguanine DNA glycosylase(EC 3.2.2.-); DNA-(apurinic or apyrimidinic site) lyase (EC 4.2.99.18)(AP lyase)].
Molecular weight: 24827
View which proteins in this organism that is involved with DNA Repair;
classified after biological processes (using data from the GOA project):
DNA repair( GO:0006281 ) base-excision repair( GO:0006284 )
Important dates:
01-NOV-1997, integrated into UniProtKB/Swiss-Prot.
01-NOV-1996, sequence version 1.
07-MAR-2006, entry version 36.
Phylogenetic order:
Archaea Euryarchaeota Methanococci Methanococcales Methanocaldococcaceae Methanocaldococcus.
To calculate the pI (Isoelectric point - the pH where a protein has a neutral charge),
go to this page and enter the protein ID (e.g 3MG_ECOLI): http://us.expasy.org/tools/pi_tool.html
Links to references in other databases for protein OGG1_METJA:
| Database | Pointer | Add. info#1 | Add. info#2 |
| EMBL | L77117 | AAB98720.1 | - |
| PIR | D64390 | D64390. | |
| GenomeReviews | L77117_GR | MJ0724.1 | |
| TIGR | MJ0724 | -. | |
| BioCyc | MJAN243232:MJ0724-MONOMER | -.1 | |
| HAMAP | MF_00241 | - | 1. |
| InterPro | IPR003265 | Endo_3c. | |
| InterPro | IPR012092 | Thrmst_ogg. | |
| Pfam | PF00730 | HhH-GPD | 1. |
| PIRSF | PIRSF005954 | Thrmst_ogg | 1. |
| SMART | SM00478 | ENDO3c | 1. |
Keywords:
Complete proteome; DNA damage; DNA repair; Glycosidase; Hydrolase; Lyase; Multifunctional enzyme; Nuclease.
References:
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=JAL-1 / DSM 2661 / ATCC 43067;
RX MEDLINE=96337999; PubMed=8688087;
RA Bult C.J., White O., Olsen G.J., Zhou L., Fleischmann R.D.,
RA Sutton G.G., Blake J.A., FitzGerald L.M., Clayton R.A., Gocayne J.D.,
RA Kerlavage A.R., Dougherty B.A., Tomb J.-F., Adams M.D., Reich C.I.,
RA Overbeek R., Kirkness E.F., Weinstock K.G., Merrick J.M., Glodek A.,
RA Scott J.L., Geoghagen N.S.M., Weidman J.F., Fuhrmann J.L., Nguyen D.,
RA Utterback T.R., Kelley J.M., Peterson J.D., Sadow P.W., Hanna M.C.,
RA Cotton M.D., Roberts K.M., Hurst M.A., Kaine B.P., Borodovsky M.,
RA Klenk H.-P., Fraser C.M., Smith H.O., Woese C.R., Venter J.C.;
RT "Complete genome sequence of the methanogenic archaeon, Methanococcus
RT jannaschii.";
RL Science 273:1058-1073(1996).
RN [2]
RP CHARACTERIZATION, AND MUTAGENESIS OF LYS-129.
RX MEDLINE=99452928; PubMed=10521423; DOI=10.1074/jbc.274.43.30447;
RA Gogos A., Clarke N.D.;
RT "Characterization of an 8-oxoguanine DNA glycosylase from
RT Methanococcus jannaschii.";
RL J. Biol. Chem. 274:30447-30450(1999).
Feature:
CHAIN 1 207 N-glycosylase/DNA lyase.
/FTId=PRO_0000159562.
ACT_SITE 129 129
MUTAGEN 129 129 K->S: Loss of activity.
Comments:
-!- FUNCTION: Responsible for removing an oxidatively damaged form of
guanine (7,8-dihydro-8-oxoguanine = 7-oxoG) from DNA. Also nicks
DNA at apurinic/apyrimidinic sites (AP sites). Has little
specificity for the base opposite oxoG.
-!- CATALYTIC ACTIVITY: The C-O-P bond 3' to the apurinic or
apyrimidinic site in DNA is broken by a beta-elimination reaction,
leaving a 3'-terminal unsaturated sugar and a product with a
terminal 5'-phosphate.
-!- BIOPHYSICOCHEMICAL PROPERTIES:
pH dependence:
Optimum pH is 8.5;
-!- SIMILARITY: Belongs to the type-2 OGG1 family.
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Sequence length: 207
MMLIKKIEEL KNSEIKDIID KRIQEFKSFK NKSNEEWFKE LCFCILTANF TAEGGIRIQK
EIGDGFLTLP REELEEKLKN LGHRFYRKRA EYIVLARRFK NIKDIVESFE NEKVAREFLV
RNIKGIGYKE ASHFLRNVGY DDVAIIDRHI LRELYENNYI DEIPKTLSRR KYLEIENILR
DIGEEVNLKL SELDLYIWYL RTGKVLK