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Description:
Methylated-DNA--protein-cysteine methyltransferase (EC 2.1.1.63) (6-O-methylguanine-DNA methyltransferase) (MGMT) (O-6-methylguanine-DNA-alkyltransferase).
Molecular weight: 19431
View which proteins in this organism that is involved with DNA Repair;
classified after biological processes (using data from the GOA project):
DNA repair( GO:0006281 ) DNA dealkylation( GO:0006307 )
Important dates:
16-JAN-2004, integrated into UniProtKB/Swiss-Prot.
01-NOV-1996, sequence version 1.
07-MAR-2006, entry version 33.
Phylogenetic order:
Archaea Euryarchaeota Methanococci Methanococcales Methanocaldococcaceae Methanocaldococcus.
To calculate the pI (Isoelectric point - the pH where a protein has a neutral charge),
go to this page and enter the protein ID (e.g 3MG_ECOLI): http://us.expasy.org/tools/pi_tool.html
Links to references in other databases for protein OGT_METJA:
| Database | Pointer | Add. info#1 | Add. info#2 |
| EMBL | L77117 | AAB99547.1 | - |
| PIR | H64490 | H64490. | |
| HSSP | O74023 | 1MGT | |
| GenomeReviews | L77117_GR | MJ1529.1 | |
| TIGR | MJ1529 | -. | |
| BioCyc | MJAN243232:MJ1529-MONOMER | -.1 | |
| HAMAP | MF_00772 | - | 1. |
| InterPro | IPR001497 | Methyltransf_1. | |
| InterPro | IPR011991 | Wing_hlx_DNA_bd. | |
| Pfam | PF01035 | DNA_binding_1 | 1. |
| TIGRFAMs | TIGR00589 | ogt | 1. |
| PROSITE | PS00374 | MGMT | 1. |
Keywords:
Complete proteome; DNA damage; DNA repair; Methyltransferase; Transferase.
References:
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=JAL-1 / DSM 2661 / ATCC 43067;
RX MEDLINE=96337999; PubMed=8688087;
RA Bult C.J., White O., Olsen G.J., Zhou L., Fleischmann R.D.,
RA Sutton G.G., Blake J.A., FitzGerald L.M., Clayton R.A., Gocayne J.D.,
RA Kerlavage A.R., Dougherty B.A., Tomb J.-F., Adams M.D., Reich C.I.,
RA Overbeek R., Kirkness E.F., Weinstock K.G., Merrick J.M., Glodek A.,
RA Scott J.L., Geoghagen N.S.M., Weidman J.F., Fuhrmann J.L., Nguyen D.,
RA Utterback T.R., Kelley J.M., Peterson J.D., Sadow P.W., Hanna M.C.,
RA Cotton M.D., Roberts K.M., Hurst M.A., Kaine B.P., Borodovsky M.,
RA Klenk H.-P., Fraser C.M., Smith H.O., Woese C.R., Venter J.C.;
RT "Complete genome sequence of the methanogenic archaeon, Methanococcus
RT jannaschii.";
RL Science 273:1058-1073(1996).
Feature:
CHAIN 1 167 Methylated-DNA--protein-cysteine
methyltransferase.
/FTId=PRO_0000139377.
ACT_SITE 128 128 Alkyl group acceptor (By similarity).
Comments:
-!- FUNCTION: Involved in the cellular defense against the biological
effects of O6-methylguanine (O6-MeG) in DNA. Repairs alkylated
guanine in DNA by stoichiometrically transferring the alkyl group
at the O-6 position to a cysteine residue in the enzyme. This is a
suicide reaction: the enzyme is irreversibly inactivated (By
similarity).
-!- CATALYTIC ACTIVITY: DNA (containing 6-O-methylguanine) + protein
L-cysteine = DNA (without 6-O-methylguanine) + protein S-methyl-L-
cysteine.
-!- SUBCELLULAR LOCATION: Cytoplasm (By similarity).
-!- SIMILARITY: Belongs to the MGMT family.
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Sequence length: 167
MIIQIEEYFI GMIFKGNQLV RNTIPLRREE IFNFMDGEVV SNPEDEHLKV AEIILKLYFA
EIDDKKVREL ISYKLEVPEF TKKVLDIVKD IEFGKTLTYG DIAKKLNTSP RAVGMALKRN
PLPLIIPCHR VVAKNSLGGY SYGLDKKKFI LERERLNMVS FKFNKVY