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Description:
Flap structure-specific endonuclease (EC 3.1.-.-).
Molecular weight: 37711
View which proteins in this organism that is involved with DNA Repair;
classified after biological processes (using data from the GOA project):
DNA repair( GO:0006281 )
Important dates:
12-FEB-2003, integrated into UniProtKB/Swiss-Prot.
01-OCT-2002, sequence version 1.
07-MAR-2006, entry version 21.
Phylogenetic order:
Archaea Euryarchaeota Methanomicrobia Methanosarcinales Methanosarcinaceae Methanosarcina.
To calculate the pI (Isoelectric point - the pH where a protein has a neutral charge),
go to this page and enter the protein ID (e.g 3MG_ECOLI): http://us.expasy.org/tools/pi_tool.html
Links to references in other databases for protein FEN_METMA:
| Database | Pointer | Add. info#1 | Add. info#2 |
| EMBL | AE008384 | AAM30602.1 | - |
| HSSP | O93634 | 1B43 | |
| GenomeReviews | AE008384_GR | MM0906.1 | |
| BioCyc | MMAZ192952:MM0906-MONOMER | -.1 | |
| HAMAP | MF_00614 | - | 1. |
| InterPro | IPR000513 | Exo_N_I. | |
| InterPro | IPR008918 | HhH2. | |
| InterPro | IPR006086 | XPG_I. | |
| InterPro | IPR006085 | XPG_N. | |
| InterPro | IPR006084 | XPGC_Rad. | |
| Pfam | PF00867 | XPG_I | 1. |
| Pfam | PF00752 | XPG_N | 1. |
| PRINTS | PR00853 | XPGRADSUPER. | |
| SMART | SM00279 | HhH2 | 1. |
| SMART | SM00484 | XPGI | 1. |
| SMART | SM00485 | XPGN | 1. |
| PROSITE | PS00841 | XPG_1 | 1. |
Keywords:
Complete proteome; Endonuclease; Hydrolase; Magnesium; Metal-binding; Nuclease.
References:
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Goe1 / Go1 / ATCC BAA-199 / DSM 3647 / OCM 88;
RX MEDLINE=22120827; PubMed=12125824;
RA Deppenmeier U., Johann A., Hartsch T., Merkl R., Schmitz R.A.,
RA Martinez-Arias R., Henne A., Wiezer A., Baeumer S., Jacobi C.,
RA Brueggemann H., Lienard T., Christmann A., Boemecke M., Steckel S.,
RA Bhattacharyya A., Lykidis A., Overbeek R., Klenk H.-P., Gunsalus R.P.,
RA Fritz H.-J., Gottschalk G.;
RT "The genome of Methanosarcina mazei: evidence for lateral gene
RT transfer between Bacteria and Archaea.";
RL J. Mol. Microbiol. Biotechnol. 4:453-461(2002).
Feature:
CHAIN 1 338 Flap structure-specific endonuclease.
/FTId=PRO_0000154055.
METAL 154 154 Magnesium 1 (By similarity).
Comments:
-!- FUNCTION: Endonuclease that cleave the 5'overhanging flap
structure that is generated by displacement synthesis when DNA
polymerase encounters the 5'end of a downstream Okazaki fragment.
Has 5'endo-/exonuclease and 5'pseudo-Y-endonuclease activities.
Cleaves the junction between single and double-stranded regions of
flap DNA (By similarity).
-!- COFACTOR: Binds 2 magnesium ions per subunit (By similarity).
-!- SIMILARITY: Belongs to the XPG/RAD2 endonuclease family. FEN1
subfamily.
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Sequence length: 338
MGTDIGDLLQ KRKIELSDLS NRVVAVDAFN TLHQFLSIIR QRDGSPLVNS QGKVTSHLSG
LLYRTASLVE AGIKPVFVFD GKPPEMKTGT LNRRKEIRES SKEKWENAKA EGNLEAAYKY
AQASSKVDQD IIEDSKYLLD IMGIPWIQAP CEGEAQAAHM VLKKDADCVA SQDYDSFLFG
APTVVRNLAA TGKRKLPGKN VYVDVELEMI ELEETLDSLG INRDQLIDIA ICVGTDYNKG
LEKVGPKTAL KLIKKHGNIH AVIREKGMEI EALDSIKELF THPDVTDDYE IKWGKPDSEK
LINFLCDEND FSEDRVVKAA DRLKAASGAR QQTLDQWF