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Description:
UvrABC system protein B (Protein uvrB) (Excinuclease ABC subunit B).
Molecular weight: 77691
View which proteins in this organism that is involved with DNA Repair;
classified after biological processes (using data from the GOA project):
DNA repair( GO:0006281 ) base-excision repair( GO:0006284 ) nucleotide-excision repair (and GO:0045001, a synonym)( GO:0006289 )
Important dates:
10-OCT-2002, integrated into UniProtKB/Swiss-Prot.
10-OCT-2002, sequence version 1.
07-MAR-2006, entry version 23.
Phylogenetic order:
Archaea Euryarchaeota Methanomicrobia Methanosarcinales Methanosarcinaceae Methanosarcina.
To calculate the pI (Isoelectric point - the pH where a protein has a neutral charge),
go to this page and enter the protein ID (e.g 3MG_ECOLI): http://us.expasy.org/tools/pi_tool.html
Links to references in other databases for protein UVRB_METMA:
| Database | Pointer | Add. info#1 | Add. info#2 |
| EMBL | AE008384 | AAM32985.1 | - |
| HSSP | P56981 | 1D9X | |
| GenomeReviews | AE008384_GR | MM3289.1 | |
| BioCyc | MMAZ192952:MM3289-MONOMER | -.1 | |
| HAMAP | MF_00204 | - | 1. |
| InterPro | IPR001410 | DEAD. | |
| InterPro | IPR011545 | DEAD/DEAH_N. | |
| InterPro | IPR001650 | Helicase_C. | |
| InterPro | IPR006935 | ResIII. | |
| InterPro | IPR001943 | UvrB/C. | |
| InterPro | IPR004807 | UvrB_ABC. | |
| Pfam | PF00271 | Helicase_C | 1. |
| Pfam | PF04851 | ResIII | 1. |
| Pfam | PF02151 | UVR | 1. |
| SMART | SM00487 | DEXDc | 1. |
| SMART | SM00490 | HELICc | 1. |
| TIGRFAMs | TIGR00631 | uvrb | 1. |
| PROSITE | PS50151 | UVR | 1. |
Keywords:
ATP-binding; Complete proteome; DNA damage; DNA excision; DNA repair; Excision nuclease; Nucleotide-binding; SOS response.
References:
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Goe1 / Go1 / ATCC BAA-199 / DSM 3647 / OCM 88;
RX MEDLINE=22120827; PubMed=12125824;
RA Deppenmeier U., Johann A., Hartsch T., Merkl R., Schmitz R.A.,
RA Martinez-Arias R., Henne A., Wiezer A., Baeumer S., Jacobi C.,
RA Brueggemann H., Lienard T., Christmann A., Boemecke M., Steckel S.,
RA Bhattacharyya A., Lykidis A., Overbeek R., Klenk H.-P., Gunsalus R.P.,
RA Fritz H.-J., Gottschalk G.;
RT "The genome of Methanosarcina mazei: evidence for lateral gene
RT transfer between Bacteria and Archaea.";
RL J. Mol. Microbiol. Biotechnol. 4:453-461(2002).
Feature:
CHAIN 1 670 UvrABC system protein B.
/FTId=PRO_0000138455.
DOMAIN 631 666 UVR.
NP_BIND 64 71 ATP (Potential).
MOTIF 117 140 Beta-hairpin.
Comments:
-!- FUNCTION: The UvrABC repair system catalyzes the recognition and
processing of DNA lesions. A damage recognition complex composed
of 2 uvrA and 2 uvrB subunits scans DNA for abnormalities. Upon
binding of the uvrA(2)B(2) complex to a putative damaged site, the
DNA wraps around one uvrB monomer. DNA wrap is dependent on ATP
binding by uvrB and probably causes local melting of the DNA
helix, facilitating insertion of uvrB beta-hairpin between the DNA
strands. Then uvrB probes one DNA strand for the presence of a
lesion. If a lesion is found the uvrA subunits dissociate and the
uvrB-DNA preincision complex is formed. This complex is
subsequently bound by uvrC and the second uvrB is released. If no
lesion is found, the DNA wraps around the other uvrB subunit that
will check the other stand for damage (By similarity).
-!- SUBUNIT: Forms a heterotetramer with uvrA during the search for
lesions. Interacts with uvrC in an incision complex (By
similarity).
-!- SUBCELLULAR LOCATION: Cytoplasm (By similarity).
-!- DOMAIN: The beta-hairpin motif is involved in DNA binding (By
similarity).
-!- SIMILARITY: Belongs to the uvrB family.
-!- SIMILARITY: Contains 1 UVR domain.
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Sequence length: 670
MKVSSQTTGE LAENKFETIY DERYWESPPF KLVSDFEPKG SQPEAIEKLV DGLKKGEPFQ
TLLGVTGSGK TYTVANVINQ IRKPTLVIAH NKTLAAQLYN EFREFFPENR VEYFVSYYDY
YQPESYLPAK DQYIEKDAMI NPKIEQMRLA ATASLMSRQD VIVVASVSCI YGLGNPENFQ
KMGFELKVGD KVHRKEILEK LIDIQFERND LELMPGRFRV KGDTIDIIPG YFDDIIRIEL
FGDEVDRISE VDKQTGQRKE DMDYFFVYPA RHYVIPEEEQ KSAIQFILEE LEERLPELGL
LESHRLKQRT LYDMEMIQET GSCKGIENYS RHFDHRQPGE KPFCLLDYFP DDFLLVIDES
HQTIPQLHGM YNGDRSRKKS LVDYGFRLPS AFDNRPLKFE EFEKYMKNVI FVSATPADYE
REHSSRVVEQ IIRPTGLVDP EVEVRPLEGQ VRDVMQEIRK IVERGDRALV TTLTKKLAEE
LTEFLAKNEI KARYLHSDIK TIERTEIIRE LRLGKFDVLV GINLLREGLD IPEVGFIGIL
DADKEGFLRN SKSLIQIIGR AARNSSSRVV LYADNMTDSI KSAVQETERR RSMQIAYNEE
HGIVPTTIRK PIREKVVDIT DTKHIPKTDI PNVIIELDAE MREAADRLDF ERAIQVRELI
KKLEKEIKAV