Description:
Methylated-DNA--protein-cysteine methyltransferase (EC 2.1.1.63) (6-O-methylguanine-DNA methyltransferase) (MGMT) (O-6-methylguanine-DNA-alkyltransferase).
Molecular weight: 17337
View which proteins in this organism that is involved with DNA Repair;
classified after biological processes (using data from the GOA project):
DNA repair( GO:0006281 ) DNA dealkylation( GO:0006307 )
Important dates:
10-MAY-2004, integrated into UniProtKB/Swiss-Prot.
01-JUN-2002, sequence version 1.
07-MAR-2006, entry version 20.
Phylogenetic order:
Archaea Euryarchaeota Methanomicrobia Methanosarcinales Methanosarcinaceae Methanosarcina.
To calculate the pI (Isoelectric point - the pH where a protein has a neutral charge),
go to this page and enter the protein ID (e.g 3MG_ECOLI): http://us.expasy.org/tools/pi_tool.html
Links to references in other databases for protein OGT_METAC:
| Database | Pointer | Add. info#1 | Add. info#2 |
| EMBL | AE011150 | AAM07666.1 | - |
| HSSP | P06134 | 1SFE | |
| GenomeReviews | AE010299_GR | MA4322.1 | |
| BioCyc | MACE188937:MA4322-MONOMER | -.1 | |
| HAMAP | MF_00772 | - | 1. |
| InterPro | IPR008332 | MethylG_mtfrase. | |
| InterPro | IPR001497 | Methyltransf_1. | |
| InterPro | IPR011991 | Wing_hlx_DNA_bd. | |
| Pfam | PF01035 | DNA_binding_1 | 1. |
| Pfam | PF02870 | Methyltransf_1N | 1. |
| TIGRFAMs | TIGR00589 | ogt | 1. |
| PROSITE | PS00374 | MGMT | 1. |
Keywords:
Complete proteome; DNA damage; DNA repair; Methyltransferase; Transferase.
References:
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=C2A / ATCC 35395 / DSM 2834;
RX MEDLINE=21929760; PubMed=11932238; DOI=10.1101/gr.223902;
RA Galagan J.E., Nusbaum C., Roy A., Endrizzi M.G., Macdonald P.,
RA FitzHugh W., Calvo S., Engels R., Smirnov S., Atnoor D., Brown A.,
RA Allen N., Naylor J., Stange-Thomann N., DeArellano K., Johnson R.,
RA Linton L., McEwan P., McKernan K., Talamas J., Tirrell A., Ye W.,
RA Zimmer A., Barber R.D., Cann I., Graham D.E., Grahame D.A., Guss A.M.,
RA Hedderich R., Ingram-Smith C., Kuettner H.C., Krzycki J.A.,
RA Leigh J.A., Li W., Liu J., Mukhopadhyay B., Reeve J.N., Smith K.,
RA Springer T.A., Umayam L.A., White O., White R.H., de Macario E.C.,
RA Ferry J.G., Jarrell K.F., Jing H., Macario A.J.L., Paulsen I.T.,
RA Pritchett M., Sowers K.R., Swanson R.V., Zinder S.H., Lander E.,
RA Metcalf W.W., Birren B.;
RT "The genome of Methanosarcina acetivorans reveals extensive metabolic
RT and physiological diversity.";
RL Genome Res. 12:532-542(2002).
Feature:
CHAIN 1 156 Methylated-DNA--protein-cysteine
methyltransferase.
/FTId=PRO_0000139376.
ACT_SITE 126 126 Alkyl group acceptor (By similarity).
Comments:
-!- FUNCTION: Involved in the cellular defense against the biological
effects of O6-methylguanine (O6-MeG) in DNA. Repairs alkylated
guanine in DNA by stoichiometrically transferring the alkyl group
at the O-6 position to a cysteine residue in the enzyme. This is a
suicide reaction: the enzyme is irreversibly inactivated (By
similarity).
-!- CATALYTIC ACTIVITY: DNA (containing 6-O-methylguanine) + protein
L-cysteine = DNA (without 6-O-methylguanine) + protein S-methyl-L-
cysteine.
-!- SUBCELLULAR LOCATION: Cytoplasm (By similarity).
-!- SIMILARITY: Belongs to the MGMT family.
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Sequence length: 156
MYYAIFESPI GPILLAGDEE GLKYVNFMKG KKKIEVPDSW VENEEFFREA SRQLEAYFAG
ELKSFDVKLA PEGTEFQKSV WNALKEIPYG ETRTYGEIAK NIGNPKASRA VGLANNRNPI
AIIVPCHRVI GANGKLTGYA SGLDIKEFLL KLEGSC