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Description:
Flap structure-specific endonuclease (EC 3.1.-.-).
Molecular weight: 38065
View which proteins in this organism that is involved with DNA Repair;
classified after biological processes (using data from the GOA project):
DNA repair( GO:0006281 )
Important dates:
12-FEB-2003, integrated into UniProtKB/Swiss-Prot.
01-JAN-1998, sequence version 1.
07-MAR-2006, entry version 37.
Phylogenetic order:
Archaea Euryarchaeota Archaeoglobi Archaeoglobales Archaeoglobaceae Archaeoglobus.
To calculate the pI (Isoelectric point - the pH where a protein has a neutral charge),
go to this page and enter the protein ID (e.g 3MG_ECOLI): http://us.expasy.org/tools/pi_tool.html
Links to references in other databases for protein FEN_ARCFU:
| Database | Pointer | Add. info#1 | Add. info#2 |
| EMBL | AE000782 | AAB90967.1 | - |
| PIR | H69282 | H69282. | |
| PDB | 1RXV | X-ray | A/B=1-336. |
| PDB | 1RXW | X-ray | A=1-336. |
| PDB | 1RXZ | X-ray | B=326-336. |
| GenomeReviews | AE000782_GR | AF0264.1 | |
| TIGR | AF0264 | -. | |
| BioCyc | AFUL2234:AF0264-MONOMER | -.1 | |
| HAMAP | MF_00614 | - | 1. |
| InterPro | IPR002421 | 5_3_exonuclease. | |
| InterPro | IPR000513 | Exo_N_I. | |
| InterPro | IPR008918 | HhH2. | |
| InterPro | IPR006086 | XPG_I. | |
| InterPro | IPR006085 | XPG_N. | |
| InterPro | IPR006084 | XPGC_Rad. | |
| Pfam | PF00867 | XPG_I | 1. |
| Pfam | PF00752 | XPG_N | 1. |
| PRINTS | PR00853 | XPGRADSUPER. | |
| SMART | SM00279 | HhH2 | 1. |
| SMART | SM00484 | XPGI | 1. |
| SMART | SM00485 | XPGN | 1. |
| PROSITE | PS00841 | XPG_1 | 1. |
Keywords:
3D-structure; Complete proteome; Endonuclease; Hydrolase; Magnesium; Metal-binding; Nuclease.
References:
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=VC-16 / DSM 4304 / ATCC 49558;
RX MEDLINE=98049343; PubMed=9389475; DOI=10.1038/37052;
RA Klenk H.-P., Clayton R.A., Tomb J.-F., White O., Nelson K.E.,
RA Ketchum K.A., Dodson R.J., Gwinn M.L., Hickey E.K., Peterson J.D.,
RA Richardson D.L., Kerlavage A.R., Graham D.E., Kyrpides N.C.,
RA Fleischmann R.D., Quackenbush J., Lee N.H., Sutton G.G., Gill S.R.,
RA Kirkness E.F., Dougherty B.A., McKenney K., Adams M.D., Loftus B.J.,
RA Peterson S.N., Reich C.I., McNeil L.K., Badger J.H., Glodek A.,
RA Zhou L., Overbeek R., Gocayne J.D., Weidman J.F., McDonald L.A.,
RA Utterback T.R., Cotton M.D., Spriggs T., Artiach P., Kaine B.P.,
RA Sykes S.M., Sadow P.W., D'Andrea K.P., Bowman C., Fujii C.,
RA Garland S.A., Mason T.M., Olsen G.J., Fraser C.M., Smith H.O.,
RA Woese C.R., Venter J.C.;
RT "The complete genome sequence of the hyperthermophilic, sulphate-
RT reducing archaeon Archaeoglobus fulgidus.";
RL Nature 390:364-370(1997).
Feature:
CHAIN 1 336 Flap structure-specific endonuclease.
/FTId=PRO_0000154050.
METAL 153 153 Magnesium 1 (By similarity).
TURN 4 4
HELIX 5 8
STRAND 12 13
HELIX 16 19
TURN 20 21
STRAND 23 27
HELIX 28 38
STRAND 39 40
TURN 42 43
STRAND 44 44
STRAND 46 46
STRAND 48 48
TURN 50 51
STRAND 52 52
STRAND 54 54
HELIX 56 71
TURN 72 72
STRAND 74 79
STRAND 82 82
HELIX 85 111
TURN 112 112
TURN 114 115
HELIX 116 123
HELIX 128 140
TURN 141 142
STRAND 145 147
STRAND 149 150
HELIX 152 161
TURN 162 163
STRAND 164 169
STRAND 171 173
HELIX 174 177
TURN 178 179
STRAND 181 186
STRAND 207 210
HELIX 211 218
TURN 219 219
HELIX 222 232
STRAND 234 234
TURN 235 236
STRAND 237 237
TURN 241 242
HELIX 245 255
STRAND 256 257
HELIX 258 265
TURN 266 266
HELIX 273 280
STRAND 281 281
HELIX 297 304
TURN 305 308
HELIX 312 322
Comments:
-!- FUNCTION: Endonuclease that cleave the 5'overhanging flap
structure that is generated by displacement synthesis when DNA
polymerase encounters the 5'end of a downstream Okazaki fragment.
Has 5'endo-/exonuclease and 5'pseudo-Y-endonuclease activities.
Cleaves the junction between single and double-stranded regions of
flap DNA (By similarity).
-!- COFACTOR: Binds 2 magnesium ions per subunit (By similarity).
-!- SIMILARITY: Belongs to the XPG/RAD2 endonuclease family. FEN1
subfamily.
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Sequence length: 336
MGADIGDLFE REEVELEYFS GKKIAVDAFN TLYQFISIIR QPDGTPLKDS QGRITSHLSG
ILYRVSNMVE VGIRPVFVFD GEPPEFKKAE IEERKKRRAE AEEMWIAALQ AGDKDAKKYA
QAAGRVDEYI VDSAKTLLSY MGIPFVDAPS EGEAQAAYMA AKGDVEYTGS QDYDSLLFGS
PRLARNLAIT GKRKLPGKNV YVDVKPEIII LESNLKRLGL TREQLIDIAI LVGTDYNEGV
KGVGVKKALN YIKTYGDIFR ALKALKVNID HVEEIRNFFL NPPVTDDYRI EFREPDFEKA
IEFLCEEHDF SRERVEKALE KLKALKSTQA TLERWF