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Description:
Methylated-DNA--protein-cysteine methyltransferase (EC 2.1.1.63) (6-O-methylguanine-DNA methyltransferase) (MGMT) (O-6-methylguanine-DNA-alkyltransferase).
Molecular weight: 16717
View which proteins in this organism that is involved with DNA Repair;
classified after biological processes (using data from the GOA project):
DNA repair( GO:0006281 ) DNA dealkylation( GO:0006307 )
Important dates:
10-MAY-2004, integrated into UniProtKB/Swiss-Prot.
01-JAN-1998, sequence version 1.
07-MAR-2006, entry version 30.
Phylogenetic order:
Archaea Euryarchaeota Archaeoglobi Archaeoglobales Archaeoglobaceae Archaeoglobus.
To calculate the pI (Isoelectric point - the pH where a protein has a neutral charge),
go to this page and enter the protein ID (e.g 3MG_ECOLI): http://us.expasy.org/tools/pi_tool.html
Links to references in other databases for protein OGT_ARCFU:
| Database | Pointer | Add. info#1 | Add. info#2 |
| EMBL | AE000782 | AAB88942.1 | - |
| PIR | B69539 | B69539. | |
| HSSP | O74023 | 1MGT | |
| GenomeReviews | AE000782_GR | AF2314.1 | |
| TIGR | AF2314 | -. | |
| BioCyc | AFUL2234:AF2314-MONOMER | -.1 | |
| HAMAP | MF_00772 | - | 1. |
| InterPro | IPR001497 | Methyltransf_1. | |
| InterPro | IPR011991 | Wing_hlx_DNA_bd. | |
| Pfam | PF01035 | DNA_binding_1 | 1. |
| TIGRFAMs | TIGR00589 | ogt | 1. |
| PROSITE | PS00374 | MGMT | 1. |
Keywords:
Complete proteome; DNA damage; DNA repair; Methyltransferase; Transferase.
References:
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=VC-16 / DSM 4304 / ATCC 49558;
RX MEDLINE=98049343; PubMed=9389475; DOI=10.1038/37052;
RA Klenk H.-P., Clayton R.A., Tomb J.-F., White O., Nelson K.E.,
RA Ketchum K.A., Dodson R.J., Gwinn M.L., Hickey E.K., Peterson J.D.,
RA Richardson D.L., Kerlavage A.R., Graham D.E., Kyrpides N.C.,
RA Fleischmann R.D., Quackenbush J., Lee N.H., Sutton G.G., Gill S.R.,
RA Kirkness E.F., Dougherty B.A., McKenney K., Adams M.D., Loftus B.J.,
RA Peterson S.N., Reich C.I., McNeil L.K., Badger J.H., Glodek A.,
RA Zhou L., Overbeek R., Gocayne J.D., Weidman J.F., McDonald L.A.,
RA Utterback T.R., Cotton M.D., Spriggs T., Artiach P., Kaine B.P.,
RA Sykes S.M., Sadow P.W., D'Andrea K.P., Bowman C., Fujii C.,
RA Garland S.A., Mason T.M., Olsen G.J., Fraser C.M., Smith H.O.,
RA Woese C.R., Venter J.C.;
RT "The complete genome sequence of the hyperthermophilic, sulphate-
RT reducing archaeon Archaeoglobus fulgidus.";
RL Nature 390:364-370(1997).
Feature:
CHAIN 1 147 Methylated-DNA--protein-cysteine
methyltransferase.
/FTId=PRO_0000139375.
ACT_SITE 112 112 Alkyl group acceptor (By similarity).
Comments:
-!- FUNCTION: Involved in the cellular defense against the biological
effects of O6-methylguanine (O6-MeG) in DNA. Repairs alkylated
guanine in DNA by stoichiometrically transferring the alkyl group
at the O-6 position to a cysteine residue in the enzyme. This is a
suicide reaction: the enzyme is irreversibly inactivated (By
similarity).
-!- CATALYTIC ACTIVITY: DNA (containing 6-O-methylguanine) + protein
L-cysteine = DNA (without 6-O-methylguanine) + protein S-methyl-L-
cysteine.
-!- SUBCELLULAR LOCATION: Cytoplasm (By similarity).
-!- SIMILARITY: Belongs to the MGMT family.
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Sequence length: 147
MFSVKWGELY FNVVMEGGKA VKSYFSTYPS FSSSDSEYAR QLERYFSGER VEVRIPYRLK
ASSFTRRVLE EVSRIPYGMV RMYSDIAKAL NTSPRAVGQA VKRNPLPVII PCHRVVGKKE
IGGYTVSCSD IDGKSLKKRL LRLEGVF