Description:
Thermostable DNA ligase (EC 6.5.1.1) (Polydeoxyribonucleotide synthase[ATP]) (Pfu DNA ligase).
Molecular weight: 63773
View which proteins in this organism that is involved with DNA Repair;
classified after biological processes (using data from the GOA project):
DNA repair( GO:0006281 )
Important dates:
30-MAY-2000, integrated into UniProtKB/Swiss-Prot.
30-MAY-2000, sequence version 1.
07-MAR-2006, entry version 39.
Phylogenetic order:
Archaea Euryarchaeota Thermococci Thermococcales Thermococcaceae Pyrococcus.
To calculate the pI (Isoelectric point - the pH where a protein has a neutral charge),
go to this page and enter the protein ID (e.g 3MG_ECOLI): http://us.expasy.org/tools/pi_tool.html
Links to references in other databases for protein DNLI_PYRFU:
| Database | Pointer | Add. info#1 | Add. info#2 |
| EMBL | AE010263 | AAL81759.1 | - |
| GenomeReviews | AE009950_GR | PF1635.1 | |
| BioCyc | PFUR186497:PF1635-MONOMER | -.1 | |
| HAMAP | MF_00407 | - | 1. |
| InterPro | IPR000977 | DNA_ligase. | |
| InterPro | IPR012309 | DNA_ligase_A_C. | |
| InterPro | IPR012310 | DNA_ligase_A_M. | |
| InterPro | IPR012308 | DNA_ligase_A_N. | |
| InterPro | IPR012340 | OB_NA_bd_sub. | |
| PANTHER | PTHR10459 | DNA_ligase.1 | 1. |
| Pfam | PF04679 | DNA_ligase_A_C | 1. |
| Pfam | PF01068 | DNA_ligase_A_M | 1. |
| Pfam | PF04675 | DNA_ligase_A_N | 1. |
| TIGRFAMs | TIGR00574 | dnl1 | 1. |
| PROSITE | PS00697 | DNA_LIGASE_A1 | 1. |
| PROSITE | PS00333 | DNA_LIGASE_A2 | FALSE_NEG. |
| PROSITE | PS50160 | DNA_LIGASE_A3 | 1. |
Keywords:
ATP-binding; Cell cycle; Cell division; Complete proteome; Direct protein sequencing; DNA damage; DNA recombination; DNA repair; DNA replication; Ligase; Nucleotide-binding.
References:
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND PROTEIN SEQUENCE OF N-TERMINUS.
RC STRAIN=Vc1 / DSM 3638 / ATCC 43587 / JCM 8422;
RA Mathur E.J., Marsh E.J., Schoettlin W.E.;
RT "Purified thermostable Pyrococcus furiosus DNA ligase.";
RL Patent number US5700672, 23-DEC-1997.
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Vc1 / DSM 3638 / ATCC 43587 / JCM 8422;
RA Weiss R.B., Dunn D.M., Robb F.T., Brown J.R.;
RT "The complete sequence of the Pyrococcus furiosus genome.";
RL Submitted (FEB-2002) to the EMBL/GenBank/DDBJ databases.
Feature:
CHAIN 1 561 Thermostable DNA ligase.
/FTId=PRO_0000059613.
ACT_SITE 249 249 N6-AMP-lysine intermediate (By
similarity).
Comments:
-!- FUNCTION: This protein seals, during DNA replication, DNA
recombination and DNA repair, nicks in double-stranded DNA.
-!- CATALYTIC ACTIVITY: ATP + (deoxyribonucleotide)(n) +
(deoxyribonucleotide)(m) = AMP + diphosphate +
(deoxyribonucleotide)(n+m).
-!- BIOPHYSICOCHEMICAL PROPERTIES:
Temperature dependence:
Optimum temperature is about 70 degrees Celsius. Active from 4
to 100 degrees Celsius. Thermostable;
-!- SUBUNIT: Monomer.
-!- SIMILARITY: Belongs to the ATP-dependent DNA ligase family.
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Sequence length: 561
MRYLELAQLY QKLEKTTMKL IKTRLVADFL KKVPDDHLEF IPYLILGEVF PEWDERELGV
GEKLLIKAVA MATGIDAKEI EESVKDTGDL GESIALAVKK KKQKSFFSQP LTIKRVYQTL
VKVAETTGEG SQDKKVKYLA DLFMDAEPLE AKYLARTILG TMRTGVAEGL LRDAIAMAFH
VKVELVERAY MLTSDFGYVA KIAKLEGNEG LAKVQVQLGK PIKPMLAQQA ASIRDALLEM
GGEAEFEIKY DGARVQVHKD GSKIIVYSRR LENVTRAIPE IVEALKEAII PEKAIVEGEL
VAIGENGRPL PFQYVLRRFR RKHNIEEMME KIPLELNLFD VLYVDGQSLI DTKFIDRRRT
LEEIIKQNEK IKVAENLITK KVEEAEAFYK RALEMGHEGL MAKRLDAVYE PGNRGKKWLK
IKPTMENLDL VIIGAEWGEG RRAHLFGSFI LGAYDPETGE FLEVGKVGSG FTDDDLVEFT
KMLKPLIIKE EGKRVWLQPK VVIEVTYQEI QKSPKYRSGF ALRFPRFVAL RDDKGPEDAD
TIERIAQLYE LQEKMKGKVE S