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Description:
Probable endonuclease IV (EC 3.1.21.2) (Endodeoxyribonuclease IV).
Molecular weight: 31278
View which proteins in this organism that is involved with DNA Repair;
classified after biological processes (using data from the GOA project):
DNA repair( GO:0006281 )
Important dates:
01-NOV-2002, integrated into UniProtKB/Swiss-Prot.
01-MAR-2001, sequence version 1.
07-MAR-2006, entry version 27.
Phylogenetic order:
Archaea Euryarchaeota Thermoplasmata Thermoplasmatales Thermoplasmataceae Thermoplasma.
To calculate the pI (Isoelectric point - the pH where a protein has a neutral charge),
go to this page and enter the protein ID (e.g 3MG_ECOLI): http://us.expasy.org/tools/pi_tool.html
Links to references in other databases for protein END4_THEAC:
| Database | Pointer | Add. info#1 | Add. info#2 |
| EMBL | AL445065 | CAC12020.1 | - |
| HSSP | P12638 | 1QTW | |
| GenomeReviews | AL139299_GR | Ta0891.1 | |
| BioCyc | TACI2303:TA0891-MONOMER | -.1 | |
| HAMAP | MF_00152 | - | 1. |
| InterPro | IPR001719 | AP_endnuclease2. | |
| InterPro | IPR012307 | Xylisom_TIMbarrl. | |
| Pfam | PF01261 | AP_endonuc_2 | 1. |
| SMART | SM00518 | AP2Ec | 1. |
| TIGRFAMs | TIGR00587 | nfo | 1. |
| PROSITE | PS00729 | AP_NUCLEASE_F2_1 | 1. |
| PROSITE | PS00730 | AP_NUCLEASE_F2_2 | 1. |
| PROSITE | PS00731 | AP_NUCLEASE_F2_3 | 1. |
Keywords:
Complete proteome; DNA damage; DNA repair; Endonuclease; Hydrolase; Metal-binding; Nuclease; Zinc.
References:
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=DSM 1728;
RX MEDLINE=20479972; PubMed=11029001; DOI=10.1038/35035069;
RA Ruepp A., Graml W., Santos-Martinez M.-L., Koretke K.K., Volker C.,
RA Mewes H.-W., Frishman D., Stocker S., Lupas A.N., Baumeister W.;
RT "The genome sequence of the thermoacidophilic scavenger Thermoplasma
RT acidophilum.";
RL Nature 407:508-513(2000).
Feature:
CHAIN 1 281 Probable endonuclease IV.
/FTId=PRO_0000190894.
METAL 78 78 Zinc 1 (By similarity).
METAL 118 118 Zinc 1 (By similarity).
METAL 149 149 Zinc 1 (By similarity).
METAL 149 149 Zinc 2 (By similarity).
METAL 181 181 Zinc 2 (By similarity).
METAL 184 184 Zinc 3 (By similarity).
METAL 216 216 Zinc 2 (By similarity).
METAL 229 229 Zinc 3 (By similarity).
METAL 231 231 Zinc 3 (By similarity).
METAL 260 260 Zinc 2 (By similarity).
Comments:
-!- FUNCTION: Endonuclease IV plays a role in DNA repair. It cleaves
phosphodiester bonds at apurinic or apyrimidinic sites (AP sites)
to produce new 5' ends that are base-free deoxyribose 5-phosphate
residues. It preferentially attacks modified AP sites created by
bleomycin and neocarzinostatin (By similarity).
-!- CATALYTIC ACTIVITY: Endonucleolytic cleavage to 5'-
phosphooligonucleotide end-products.
-!- COFACTOR: Binds 3 zinc ions (By similarity).
-!- SIMILARITY: Belongs to the AP endonuclease 2 family.
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Sequence length: 281
MIDEAIRSIS KKYTIGGHIS VAGGLHNGPA RAAVFGFPTF QFFSKNQMRW SSPPLKDDEA
AAFKSEVRKY GIESTMIHAS YLINLASADP DLYKRSMEAF HDEIDRSDKL GSTFLTVHPG
SNPDRADGIR RVRDALSTIG DHAVIILIEN TAGQGNVIGT RLDEVAKIID TSDKKLGVCI
DTCHAWASGY DLRDSLEKFI ESLDYTIGLD RIFAFHLNDA KREMGSRIDR HELIGKGTID
GGLINLIRDD RLRAKPKIME TPFGEARFED NLRYMSSKIG E