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Description:
Bifunctional methyltransferase/endonuclease [Includes: Probablemethylated-DNA--protein-cysteine methyltransferase (EC 2.1.1.-) (O-6-methylbase-DNA-alkyltransferase); Endonuclease V (EC 3.1.21.7)(Deoxyinosine 3'endonuclease) (Deoxyribonuclease V) (DNase V)].
Molecular weight: 36732
View which proteins in this organism that is involved with DNA Repair;
classified after biological processes (using data from the GOA project):
DNA repair( GO:0006281 )
Important dates:
07-DEC-2004, integrated into UniProtKB/Swiss-Prot.
01-MAR-2001, sequence version 1.
07-MAR-2006, entry version 23.
Phylogenetic order:
Archaea Euryarchaeota Thermoplasmata Thermoplasmatales Thermoplasmataceae Thermoplasma.
To calculate the pI (Isoelectric point - the pH where a protein has a neutral charge),
go to this page and enter the protein ID (e.g 3MG_ECOLI): http://us.expasy.org/tools/pi_tool.html
Links to references in other databases for protein NFI_THEAC:
| Database | Pointer | Add. info#1 | Add. info#2 |
| EMBL | AL445064 | CAC11602.1 | - |
| HSSP | O74023 | 1MGT | |
| GenomeReviews | AL139299_GR | Ta0460.1 | |
| BioCyc | TACI2303:TA0460-MONOMER | -.1 | |
| LinkHub | Q9HKY3 | -.1 | |
| HAMAP | MF_00801 | fused | 1. |
| InterPro | IPR007581 | Endonuc_V. | |
| InterPro | IPR001497 | Methyltransf_1. | |
| InterPro | IPR011991 | Wing_hlx_DNA_bd. | |
| Pfam | PF01035 | DNA_binding_1 | 1. |
| Pfam | PF04493 | Endonuclease_5 | 1. |
| TIGRFAMs | TIGR00589 | ogt | 1. |
Keywords:
Complete proteome; DNA damage; DNA repair; Endonuclease; Hydrolase; Magnesium; Methyltransferase; Multifunctional enzyme; Nuclease; Transferase.
References:
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=DSM 1728;
RX MEDLINE=20479972; PubMed=11029001; DOI=10.1038/35035069;
RA Ruepp A., Graml W., Santos-Martinez M.-L., Koretke K.K., Volker C.,
RA Mewes H.-W., Frishman D., Stocker S., Lupas A.N., Baumeister W.;
RT "The genome sequence of the thermoacidophilic scavenger Thermoplasma
RT acidophilum.";
RL Nature 407:508-513(2000).
Feature:
CHAIN 1 323 Bifunctional
methyltransferase/endonuclease.
/FTId=PRO_0000159699.
REGION 1 91 Probable methylated-DNA--protein-cysteine
methyltransferase.
REGION 92 323 Endonuclease V.
ACT_SITE 66 66 Potential.
Comments:
-!- FUNCTION: Selectively cleaves double-stranded DNA at the second
phosphodiester bond 3' to a deoxyinosine leaving behind the intact
lesion on the nicked DNA. Acts in DNA repair (By similarity).
-!- CATALYTIC ACTIVITY: Endonucleolytic cleavage at apurinic or
apyrimidinic sites to products with a 5'-phosphate.
-!- COFACTOR: Magnesium (By similarity).
-!- SUBCELLULAR LOCATION: Cytoplasm (By similarity).
-!- SIMILARITY: In the N-terminal section; belongs to the MGMT family.
-!- SIMILARITY: In the C-terminal section; belongs to the endonuclease
V family.
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Sequence length: 323
MLGSIACSME FEDFDLYSYF YGLVKQIPEG YVTTYGDLAQ ALGDPIAARA VGYMLSINED
PDTIPCYRVV LHDGTVGNYT HPLGPMEKVR RLRADGIPVV DGMIENFDRY RFRDFKTDQP
LRKMQQYQEY IARKYSESGA GEFRTVAAFD VSYEERRAFA SMVIQDGSTI EAYVISDGIN
FPYIPGYLAF REFRFIKKLY RKADLLLIDG NGILHPRFAG LATHAGVLLD RASIGVAKHL
TRSTRHQDII YMQDRPVGVM IRKNMIVSPG NFIDVNGSAD TVRKMWNDRY PWPLKLAHQL
STRHAETGGR VSEYSIESAH ELH