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Description:
LexA repressor (EC 3.4.21.88).
Molecular weight: 22506
View which proteins in this organism that is involved with DNA Repair;
classified after biological processes (using data from the GOA project):
DNA repair( GO:0006281 )
Important dates:
01-OCT-1994, integrated into UniProtKB/Swiss-Prot.
01-OCT-1994, sequence version 1.
07-MAR-2006, entry version 48.
Phylogenetic order:
Bacteria Proteobacteria Gammaproteobacteria Pseudomonadales Pseudomonadaceae Pseudomonas.
To calculate the pI (Isoelectric point - the pH where a protein has a neutral charge),
go to this page and enter the protein ID (e.g 3MG_ECOLI): http://us.expasy.org/tools/pi_tool.html
Links to references in other databases for protein LEXA_PSEAE:
| Database | Pointer | Add. info#1 | Add. info#2 |
| EMBL | X63018 | CAA44750.1 | - |
| EMBL | AE004726 | AAG06395.1 | - |
| PIR | S30165 | S30165. | |
| HSSP | P03033 | 1JHH | |
| MEROPS | S24.001 | -. | |
| GenomeReviews | AE004091_GR | PA3007.1 | |
| BioCyc | PAER287:PA3007-MONOMER | -.1 | |
| HAMAP | MF_00015 | - | 1. |
| InterPro | IPR006199 | LexA_DNA_bd. | |
| InterPro | IPR006200 | Pept_S24_LexA. | |
| InterPro | IPR006198 | Pept_S24_S26. | |
| InterPro | IPR006197 | Pept_S24_SOS. | |
| InterPro | IPR013324 | Sigma_r3_r4. | |
| InterPro | IPR011991 | Wing_hlx_DNA_bd. | |
| Pfam | PF01726 | LexA_DNA_bind | 1. |
| Pfam | PF00717 | Peptidase_S24 | 1. |
| PRINTS | PR00726 | LEXASERPTASE. | |
| TIGRFAMs | TIGR00498 | lexA | 1. |
Keywords:
Autocatalytic cleavage; Complete proteome; DNA damage; DNA repair; DNA replication; DNA-binding; Hydrolase; Repressor; SOS response; Transcription; Transcription regulation.
References:
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=PAO;
RX MEDLINE=93156677; PubMed=1494343;
RA Garriga X., Calero S., Barbe J.;
RT "Nucleotide sequence analysis and comparison of the lexA genes from
RT Salmonella typhimurium, Erwinia carotovora, Pseudomonas aeruginosa and
RT Pseudomonas putida.";
RL Mol. Gen. Genet. 236:125-134(1992).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 15692 / PAO1;
RX MEDLINE=20437337; PubMed=10984043; DOI=10.1038/35023079;
RA Stover C.K., Pham X.-Q.T., Erwin A.L., Mizoguchi S.D., Warrener P.,
RA Hickey M.J., Brinkman F.S.L., Hufnagle W.O., Kowalik D.J., Lagrou M.,
RA Garber R.L., Goltry L., Tolentino E., Westbrock-Wadman S., Yuan Y.,
RA Brody L.L., Coulter S.N., Folger K.R., Kas A., Larbig K., Lim R.M.,
RA Smith K.A., Spencer D.H., Wong G.K.-S., Wu Z., Paulsen I.T.,
RA Reizer J., Saier M.H. Jr., Hancock R.E.W., Lory S., Olson M.V.;
RT "Complete genome sequence of Pseudomonas aeruginosa PAO1, an
RT opportunistic pathogen.";
RL Nature 406:959-964(2000).
Feature:
CHAIN 1 204 LexA repressor.
/FTId=PRO_0000170067.
DNA_BIND 28 48 H-T-H motif (By similarity).
ACT_SITE 125 125 Involved in auto-cleavage (By
similarity).
ACT_SITE 162 162 Involved in auto-cleavage (By
similarity).
SITE 90 91 Cleavage (auto-) (By similarity).
Comments:
-!- FUNCTION: Represses a number of genes involved in the response to
DNA damage (SOS response), including recA and lexA. In the
presence of single-stranded DNA, recA interacts with lexA causing
an autocatalytic cleavage which disrupts the DNA-binding part of
lexA, leading to derepression of the SOS regulon and eventually
DNA repair (By similarity).
-!- CATALYTIC ACTIVITY: Hydrolysis of Ala-|-Gly bond in repressor
lexA.
-!- SUBUNIT: Homodimer (By similarity).
-!- SIMILARITY: Belongs to the peptidase S24 family.
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Sequence length: 204
MQKLTPRQAE ILSFIKRCLE DHGFPPTRAE IAQELGFKSP NAAEEHLKAL ARKGAIEMTP
GASRGIRIPG FEPHAANDDE GLPVIGRVAA GAPILAEQNI EESCRINPAF FNPRADYLLR
VRGMSMKDIG ILDGDLLAVH VTREARNGQV VVARIGEEVT VKRFKREGSK VWLLAENPEF
APIEVDLKEQ ELIIEGLSVG VIRR