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Description:
Holliday junction ATP-dependent DNA helicase ruvB (EC 3.6.1.-).
Molecular weight: 38933
View which proteins in this organism that is involved with DNA Repair;
classified after biological processes (using data from the GOA project):
DNA repair( GO:0006281 )
Important dates:
01-NOV-1997, integrated into UniProtKB/Swiss-Prot.
11-JAN-2001, sequence version 2.
07-MAR-2006, entry version 40.
Phylogenetic order:
Bacteria Proteobacteria Gammaproteobacteria Pseudomonadales Pseudomonadaceae Pseudomonas.
To calculate the pI (Isoelectric point - the pH where a protein has a neutral charge),
go to this page and enter the protein ID (e.g 3MG_ECOLI): http://us.expasy.org/tools/pi_tool.html
Links to references in other databases for protein RUVB_PSEAE:
| Database | Pointer | Add. info#1 | Add. info#2 |
| EMBL | D83138 | BAA11819.1 | - |
| EMBL | AE004530 | AAG04356.1 | - |
| PIR | A83525 | A83525. | |
| HSSP | Q56313 | 1J7K | |
| GenomeReviews | AE004091_GR | PA0967.1 | |
| BioCyc | PAER287:PA0967-MONOMER | -.1 | |
| HAMAP | MF_00016 | - | 1. |
| InterPro | IPR003593 | AAA_ATPase. | |
| InterPro | IPR003959 | AAA_ATPase_centr. | |
| InterPro | IPR004605 | RuvB. | |
| InterPro | IPR008823 | RuvB_C. | |
| InterPro | IPR008824 | RuvB_N. | |
| InterPro | IPR011991 | Wing_hlx_DNA_bd. | |
| Pfam | PF00004 | AAA | 1. |
| Pfam | PF05491 | RuvB_C | 1. |
| Pfam | PF05496 | RuvB_N | 1. |
| SMART | SM00382 | AAA | 1. |
| TIGRFAMs | TIGR00635 | ruvB | 1. |
Keywords:
ATP-binding; Complete proteome; DNA damage; DNA recombination; DNA repair; Helicase; Hydrolase; Nucleotide-binding; SOS response.
References:
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=ATCC 15692 / PAO1;
RX MEDLINE=97136691; PubMed=8982068; DOI=10.1016/S0378-1119(96)00474-X;
RA Hishida T., Iwasaki H., Ishioka K., Shinagawa H.;
RT "Molecular analysis of the Pseudomonas aeruginosa genes, ruvA, ruvB
RT and ruvC, involved in processing of homologous recombination
RT intermediates.";
RL Gene 182:63-70(1996).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 15692 / PAO1;
RX MEDLINE=20437337; PubMed=10984043; DOI=10.1038/35023079;
RA Stover C.K., Pham X.-Q.T., Erwin A.L., Mizoguchi S.D., Warrener P.,
RA Hickey M.J., Brinkman F.S.L., Hufnagle W.O., Kowalik D.J., Lagrou M.,
RA Garber R.L., Goltry L., Tolentino E., Westbrock-Wadman S., Yuan Y.,
RA Brody L.L., Coulter S.N., Folger K.R., Kas A., Larbig K., Lim R.M.,
RA Smith K.A., Spencer D.H., Wong G.K.-S., Wu Z., Paulsen I.T.,
RA Reizer J., Saier M.H. Jr., Hancock R.E.W., Lory S., Olson M.V.;
RT "Complete genome sequence of Pseudomonas aeruginosa PAO1, an
RT opportunistic pathogen.";
RL Nature 406:959-964(2000).
Feature:
CHAIN 1 352 Holliday junction ATP-dependent DNA
helicase ruvB.
/FTId=PRO_0000165578.
NP_BIND 63 70 ATP (Potential).
CONFLICT 146 146 A -> G (in Ref. 1).
Comments:
-!- FUNCTION: The ruvA-ruvB complex in the presence of ATP renatures
cruciform structure in supercoiled DNA with palindromic sequence,
indicating that it may promote strand exchange reactions in
homologous recombination. RuvAB is an helicase that mediates the
Holliday junction migration by localized denaturation and
reannealing (By similarity).
-!- SUBUNIT: Forms a complex with ruvA.
-!- SIMILARITY: Belongs to the ruvB family.
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Sequence length: 352
MIEPDRLISA VSGRERDEQL DRAIRPLKLA DYIGQPSVRE QMELFIHAAR GRQEALDHTL
IFGPPGLGKT TLANIIAQEM GVSIKSTSGP VLERPGDLAA LLTNLEAGDV LFVDEIHRLS
PIVEEVLYPA MEDFQLDIMI GEGPAARSIK LDLPPFTLVG ATTRAGMLTN PLRDRFGIVQ
RLEFYNVEDL ATIVSRSAGI LGLEIEPQGA AEIAKRARGT PRIANRLLRR VRDFAEVRGQ
GDITRVIADK ALNLLDVDER GFDHLDRRLL LTMIDKFDGG PVGIDNLAAA LSEERHTIED
VLEPYLIQQG YIMRTPRGRV VTRHAYLHFG LNIPKRLGPG VTTDLFTSED GN