|
|
|
|
|
|
Description:
UvrABC system protein B (Protein uvrB) (Excinuclease ABC subunit B).
Molecular weight: 76067
View which proteins in this organism that is involved with DNA Repair;
classified after biological processes (using data from the GOA project):
DNA repair( GO:0006281 ) base-excision repair( GO:0006284 ) nucleotide-excision repair (and GO:0045001, a synonym)( GO:0006289 )
Important dates:
01-NOV-1997, integrated into UniProtKB/Swiss-Prot.
08-DEC-2000, sequence version 2.
07-MAR-2006, entry version 43.
Phylogenetic order:
Bacteria Proteobacteria Gammaproteobacteria Pseudomonadales Pseudomonadaceae Pseudomonas.
To calculate the pI (Isoelectric point - the pH where a protein has a neutral charge),
go to this page and enter the protein ID (e.g 3MG_ECOLI): http://us.expasy.org/tools/pi_tool.html
Links to references in other databases for protein UVRB_PSEAE:
| Database | Pointer | Add. info#1 | Add. info#2 |
| EMBL | X93486 | CAA63759.1 | - |
| EMBL | AE004737 | AAG06526.1 | - |
| EMBL | U50396 | AAC45869.1 | ALT_INIT |
| PIR | A83255 | A83255. | |
| HSSP | P56981 | 1D9X | |
| GenomeReviews | AE004091_GR | PA3138.1 | |
| BioCyc | PAER287:PA3138-MONOMER | -.1 | |
| HAMAP | MF_00204 | - | 1. |
| InterPro | IPR001410 | DEAD. | |
| InterPro | IPR011545 | DEAD/DEAH_N. | |
| InterPro | IPR001650 | Helicase_C. | |
| InterPro | IPR006935 | ResIII. | |
| InterPro | IPR001943 | UvrB/C. | |
| InterPro | IPR004807 | UvrB_ABC. | |
| Pfam | PF00271 | Helicase_C | 1. |
| Pfam | PF04851 | ResIII | 1. |
| Pfam | PF02151 | UVR | 1. |
| SMART | SM00487 | DEXDc | 1. |
| SMART | SM00490 | HELICc | 1. |
| TIGRFAMs | TIGR00631 | uvrb | 1. |
| PROSITE | PS50151 | UVR | 1. |
Keywords:
ATP-binding; Complete proteome; DNA damage; DNA excision; DNA repair; Excision nuclease; Nucleotide-binding; SOS response.
References:
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=ATCC 15692 / PAO1;
RX MEDLINE=96404811; PubMed=8808952;
RA Rivera E., Vila L., Barbe J.;
RT "The uvrB gene of Pseudomonas aeruginosa is not DNA damage
RT inducible.";
RL J. Bacteriol. 178:5550-5554(1996).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 15692 / PAO1;
RX MEDLINE=20437337; PubMed=10984043; DOI=10.1038/35023079;
RA Stover C.K., Pham X.-Q.T., Erwin A.L., Mizoguchi S.D., Warrener P.,
RA Hickey M.J., Brinkman F.S.L., Hufnagle W.O., Kowalik D.J., Lagrou M.,
RA Garber R.L., Goltry L., Tolentino E., Westbrock-Wadman S., Yuan Y.,
RA Brody L.L., Coulter S.N., Folger K.R., Kas A., Larbig K., Lim R.M.,
RA Smith K.A., Spencer D.H., Wong G.K.-S., Wu Z., Paulsen I.T.,
RA Reizer J., Saier M.H. Jr., Hancock R.E.W., Lory S., Olson M.V.;
RT "Complete genome sequence of Pseudomonas aeruginosa PAO1, an
RT opportunistic pathogen.";
RL Nature 406:959-964(2000).
RN [3]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-209.
RC STRAIN=ATCC 15692 / PAO1;
RX MEDLINE=97093969; PubMed=8939432;
RA Burrows L.L., Charter D.F., Lam J.S.;
RT "Molecular characterization of the Pseudomonas aeruginosa serotype O5
RT (PAO1) B-band lipopolysaccharide gene cluster.";
RL Mol. Microbiol. 22:481-495(1996).
Feature:
CHAIN 1 670 UvrABC system protein B.
/FTId=PRO_0000138418.
DOMAIN 631 666 UVR.
NP_BIND 38 45 ATP (Potential).
MOTIF 91 114 Beta-hairpin.
CONFLICT 173 173 R -> Q (in Ref. 1).
CONFLICT 406 406 V -> I (in Ref. 1).
Comments:
-!- FUNCTION: The UvrABC repair system catalyzes the recognition and
processing of DNA lesions. A damage recognition complex composed
of 2 uvrA and 2 uvrB subunits scans DNA for abnormalities. Upon
binding of the uvrA(2)B(2) complex to a putative damaged site, the
DNA wraps around one uvrB monomer. DNA wrap is dependent on ATP
binding by uvrB and probably causes local melting of the DNA
helix, facilitating insertion of uvrB beta-hairpin between the DNA
strands. Then uvrB probes one DNA strand for the presence of a
lesion. If a lesion is found the uvrA subunits dissociate and the
uvrB-DNA preincision complex is formed. This complex is
subsequently bound by uvrC and the second uvrB is released. If no
lesion is found, the DNA wraps around the other uvrB subunit that
will check the other stand for damage (By similarity).
-!- SUBUNIT: Forms a heterotetramer with uvrA during the search for
lesions. Interacts with uvrC in an incision complex (By
similarity).
-!- SUBCELLULAR LOCATION: Cytoplasm (By similarity).
-!- DOMAIN: The beta-hairpin motif is involved in DNA binding (By
similarity).
-!- SIMILARITY: Belongs to the uvrB family.
-!- SIMILARITY: Contains 1 UVR domain.
-----------------------------------------------------------------------
Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms
Distributed under the Creative Commons Attribution-NoDerivs License
-----------------------------------------------------------------------
Sequence length: 670
MDTFQLDSRF KPAGDQPEAI RQMVEGLEAG LSHQTLLGVT GSGKTFSIAN VIAQVQRPTL
VLAPNKTLAA QLYGEFKTFF PHNSVEYFVS YYDYYQPEAY VPSSDTYIEK DSSINDHIEQ
MRLSATKALL ERPDAIIVAT VSSIYGLGDP ASYLKMVLHL DRGDRIDQRE LLRRLTSLQY
TRNDMDFARA TFRVRGDVID IFPAESDLEA IRVELFDDEV ESLSAFDPLT GEVIRKLPRF
TFYPKSHYVT PRETLLEAVD QIKAELKERL DYLRNNNKLV EAQRLEQRTR FDLEMILELG
YCNGIENYSR YLSGRAPGEP PPTLYDYLPA NSLLVIDESH VSVPQVGAMF KGDRSRKETL
VEYGFRLPSA LDNRPLRFEE WEAVSPQTIF VSATPGPYEA EHAGRVIEQV VRPTGLVDPE
IEVRPAMTQV DDLLSQIRQR VAKDERVLVT TLTKRMAEDL TDYLGDHDVR VRYLHSDIDT
VERVEIIRDL RAGAFDVLVG INLLREGLDM PEVSLVAILD ADKEGFLRSE RSLIQTIGRA
ARNLHGKAIL YADNVTGSMQ RAIDETERRR AKQIAFNEAH GIVPKGVRKD IKDILEGAVV
PGARGKRKGV AKVAEESGRY ENELRSPSEI SKRIRQLEEK MYQLARDLEF EAAAQLRDEI
QTLRERLVNV