Protein data for DNLI_PYRAB:

Description:
Thermostable DNA ligase (EC 6.5.1.1) (Polydeoxyribonucleotide synthase[ATP]).

Molecular weight: 63553

View which proteins in this organism that is involved with DNA Repair;
classified after biological processes (using data from the GOA project):
DNA repair( GO:0006281 )

Important dates:
21-FEB-2001, integrated into UniProtKB/Swiss-Prot.
01-MAY-2000, sequence version 1.
07-MAR-2006, entry version 42.

Phylogenetic order:
Archaea Euryarchaeota Thermococci Thermococcales Thermococcaceae Pyrococcus.

To calculate the pI (Isoelectric point - the pH where a protein has a neutral charge),
go to this page and enter the protein ID (e.g 3MG_ECOLI): http://us.expasy.org/tools/pi_tool.html

Links to references in other databases for protein DNLI_PYRAB:

Database	Pointer	Add. info#1	Add. info#2
EMBL	AJ006532	CAC20743.1	-
EMBL	AJ248284	CAB49465.1	-
PIR	B75173	B75173.
GenomeReviews	AL096836_GR	PYRAB05430.1
HAMAP	MF_00407	-	1.
InterPro	IPR000977	DNA_ligase.
InterPro	IPR012309	DNA_ligase_A_C.
InterPro	IPR012310	DNA_ligase_A_M.
InterPro	IPR012308	DNA_ligase_A_N.
InterPro	IPR012340	OB_NA_bd_sub.
PANTHER	PTHR10459	DNA_ligase.1	1.
Pfam	PF04679	DNA_ligase_A_C	1.
Pfam	PF01068	DNA_ligase_A_M	1.
Pfam	PF04675	DNA_ligase_A_N	1.
TIGRFAMs	TIGR00574	dnl1	1.
PROSITE	PS00697	DNA_LIGASE_A1	1.
PROSITE	PS00333	DNA_LIGASE_A2	FALSE_NEG.
PROSITE	PS50160	DNA_LIGASE_A3	1.

General information about the databases mentioned above

Keywords:
ATP-binding; Cell cycle; Cell division; Complete proteome; DNA damage; DNA recombination; DNA repair; DNA replication; Ligase; Nucleotide-binding.

References:
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=ST 549;
RA Rolland J.L., Dietrich J.;
RT "Cloning and expression of a new thermostable DNA ligase from the
RT hyperthermophilic Pyrococcus abyssi sp. ST 549.";
RL Submitted (JUN-1998) to the EMBL/GenBank/DDBJ databases.
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=GE5 / Orsay;
RX MEDLINE=22511545; PubMed=12622808;
RX DOI=10.1046/j.1365-2958.2003.03381.x;
RA Cohen G.N., Barbe V., Flament D., Galperin M., Heilig R., Lecompte O.,
RA Poch O., Prieur D., Querellou J., Ripp R., Thierry J.-C.,
RA Van der Oost J., Weissenbach J., Zivanovic Y., Forterre P.;
RT "An integrated analysis of the genome of the hyperthermophilic
RT archaeon Pyrococcus abyssi.";
RL Mol. Microbiol. 47:1495-1512(2003).

Feature:
CHAIN 1 559 Thermostable DNA ligase.
/FTId=PRO_0000059612.
ACT_SITE 249 249 N6-AMP-lysine intermediate (By
similarity).
VARIANT 100 100 K -> R (in strain: ST 549).
VARIANT 292 292 T -> A (in strain: ST 549).
VARIANT 367 367 T -> A (in strain: ST 549).
VARIANT 408 408 V -> I (in strain: ST 549).
VARIANT 484 484 K -> R (in strain: ST 549).

Comments:
-!- FUNCTION: This protein seals, during DNA replication, DNA
recombination and DNA repair, nicks in double-stranded DNA (By
similarity).
-!- CATALYTIC ACTIVITY: ATP + (deoxyribonucleotide)(n) +
(deoxyribonucleotide)(m) = AMP + diphosphate +
(deoxyribonucleotide)(n+m).
-!- SIMILARITY: Belongs to the ATP-dependent DNA ligase family.
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Sequence length: 559

     MRYIELAQLY QKLEKTTMKL IKTRLVADFL KKVPEDHLEF IPYLILGDVF PEWDERELGV
     GEKLLIKAVS MATGIDSKEI ENSVKDTGDL GESIALAVKK RKQKSFFSQP LTIKRVYQTL
     VKVAETTGEG SQDKKMKYLA NLFMDAEPIE AKYIARTVLG TMRTGVAEGL LRDAISLAFN
     VKVELVERAY MLTSDFGFVA KIAKTEGNDG LAKVTIQIGK PIKPMLAQQA ANIKEALLEM
     GGEAEFEIKY DGARVQVHKD GEKVTIYSRR LENVTRAIPE IVEAIKEALK PTKAIVEGEL
     VAIGEDGRPL PFQYVLRRFR RKYNIEEMME KIPLELNLFD VLYVDGVSLI DTKFMERRKK
     LEEIVETNGK VKIAENLITK NVEEAEQFYK RALEMGHEGL MAKRLDAVYE PGNRGKKWLK
     IKPTMENLDL VIIGAEWGEG RRAHLLGSFI LGAYDPETGE FLEVGKVGSG FTDDDLVEFT
     KMLKPLIIKE EGKRVWIQPK VVIEVTYQEI QKSPKYRSGF ALRFPRYVAL REDKGPEDAD
     TIERIAQLYE LQERMKGKV