Description:
Methylated-DNA--protein-cysteine methyltransferase (EC 2.1.1.63) (6-O-methylguanine-DNA methyltransferase) (MGMT) (O-6-methylguanine-DNA-alkyltransferase).
Molecular weight: 19713
View which proteins in this organism that is involved with DNA Repair;
classified after biological processes (using data from the GOA project):
DNA repair( GO:0006281 ) DNA dealkylation( GO:0006307 )
Important dates:
10-MAY-2004, integrated into UniProtKB/Swiss-Prot.
01-MAY-2000, sequence version 1.
07-MAR-2006, entry version 24.
Phylogenetic order:
Archaea Euryarchaeota Thermococci Thermococcales Thermococcaceae Pyrococcus.
To calculate the pI (Isoelectric point - the pH where a protein has a neutral charge),
go to this page and enter the protein ID (e.g 3MG_ECOLI): http://us.expasy.org/tools/pi_tool.html
Links to references in other databases for protein OGT_PYRAB:
| Database | Pointer | Add. info#1 | Add. info#2 |
| EMBL | AJ248284 | CAB49312.1 | - |
| PIR | A75154 | A75154. | |
| HSSP | O74023 | 1MGT | |
| GenomeReviews | AL096836_GR | PYRAB03900.1 | |
| HAMAP | MF_00772 | - | 1. |
| InterPro | IPR001497 | Methyltransf_1. | |
| InterPro | IPR011991 | Wing_hlx_DNA_bd. | |
| Pfam | PF01035 | DNA_binding_1 | 1. |
| TIGRFAMs | TIGR00589 | ogt | 1. |
| PROSITE | PS00374 | MGMT | 1. |
Keywords:
Complete proteome; DNA damage; DNA repair; Methyltransferase; Transferase.
References:
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=GE5 / Orsay;
RX MEDLINE=22511545; PubMed=12622808;
RX DOI=10.1046/j.1365-2958.2003.03381.x;
RA Cohen G.N., Barbe V., Flament D., Galperin M., Heilig R., Lecompte O.,
RA Poch O., Prieur D., Querellou J., Ripp R., Thierry J.-C.,
RA Van der Oost J., Weissenbach J., Zivanovic Y., Forterre P.;
RT "An integrated analysis of the genome of the hyperthermophilic
RT archaeon Pyrococcus abyssi.";
RL Mol. Microbiol. 47:1495-1512(2003).
Feature:
CHAIN 1 172 Methylated-DNA--protein-cysteine
methyltransferase.
/FTId=PRO_0000139381.
ACT_SITE 142 142 Alkyl group acceptor (By similarity).
Comments:
-!- FUNCTION: Involved in the cellular defense against the biological
effects of O6-methylguanine (O6-MeG) in DNA. Repairs alkylated
guanine in DNA by stoichiometrically transferring the alkyl group
at the O-6 position to a cysteine residue in the enzyme. This is a
suicide reaction: the enzyme is irreversibly inactivated (By
similarity).
-!- CATALYTIC ACTIVITY: DNA (containing 6-O-methylguanine) + protein
L-cysteine = DNA (without 6-O-methylguanine) + protein S-methyl-L-
cysteine.
-!- SUBCELLULAR LOCATION: Cytoplasm (By similarity).
-!- SIMILARITY: Belongs to the MGMT family.
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Sequence length: 172
MLSCESFKIK GREIIICVIW EEGIQGIVYS LDGREFLEKQ LSRLISMLNK RGVSLSLKER
HSKYPELVFN VLTGKISNEE GFEELSLEGL TDFEIRVYSW LVKNVKRGEV ITYGKVAKEL
KTSALAIGGA MKRNPYPIVV PCHRVVGKKD PWLYTPKPEY KKFLLEVEGW TS