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Description:
DNA polymerase IV (EC 2.7.7.7) (Pol IV).
Molecular weight: 39904
View which proteins in this organism that is involved with DNA Repair;
classified after biological processes (using data from the GOA project):
DNA repair( GO:0006281 )
Important dates:
02-AUG-2002, integrated into UniProtKB/Swiss-Prot.
02-AUG-2002, sequence version 2.
07-MAR-2006, entry version 27.
Phylogenetic order:
Bacteria Proteobacteria Betaproteobacteria Burkholderiales Burkholderiaceae Ralstonia.
To calculate the pI (Isoelectric point - the pH where a protein has a neutral charge),
go to this page and enter the protein ID (e.g 3MG_ECOLI): http://us.expasy.org/tools/pi_tool.html
Links to references in other databases for protein DPO4_RALSO:
| Database | Pointer | Add. info#1 | Add. info#2 |
| EMBL | AL646065 | CAD15289.1 | ALT_INIT |
| HSSP | P96022 | 1IM4 | |
| GenomeReviews | AL646052_GR | RSc1587.1 | |
| BioCyc | RSOL305:RSC1587-MONOMER | -.1 | |
| HAMAP | MF_01113 | - | 1. |
| InterPro | IPR001126 | UMUC_like. | |
| Pfam | PF00817 | IMS | 1. |
| PROSITE | PS50173 | UMUC | 1. |
Keywords:
Complete proteome; DNA damage; DNA repair; DNA replication; DNA-binding; DNA-directed DNA polymerase; Magnesium; Metal-binding; Mutator protein; Nucleotidyltransferase; Transferase.
References:
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=GMI1000;
RX MEDLINE=21681879; PubMed=11823852; DOI=10.1038/415497a;
RA Salanoubat M., Genin S., Artiguenave F., Gouzy J., Mangenot S.,
RA Arlat M., Billault A., Brottier P., Camus J.C., Cattolico L.,
RA Chandler M., Choisne N., Claudel-Renard C., Cunnac S., Demange N.,
RA Gaspin C., Lavie M., Moisan A., Robert C., Saurin W., Schiex T.,
RA Siguier P., Thebault P., Whalen M., Wincker P., Levy M.,
RA Weissenbach J., Boucher C.A.;
RT "Genome sequence of the plant pathogen Ralstonia solanacearum.";
RL Nature 415:497-502(2002).
Feature:
CHAIN 1 357 DNA polymerase IV.
/FTId=PRO_0000173935.
DOMAIN 4 185 UmuC.
ACT_SITE 104 104 By similarity.
METAL 8 8 Magnesium (By similarity).
METAL 103 103 Magnesium (By similarity).
SITE 13 13 Substrate discrimination (By similarity).
Comments:
-!- FUNCTION: Poorly processive, error-prone DNA polymerase involved
in untargeted mutagenesis. Copies undamaged DNA at stalled
replication forks, which arise in vivo from mismatched or
misaligned primer ends. These misaligned primers can be extended
by polIV. Exhibits no 3'-5' exonuclease (proofreading) activity.
May be involved in translesional synthesis, in conjunction with
the beta clamp from polIII (By similarity).
-!- CATALYTIC ACTIVITY: Deoxynucleoside triphosphate + DNA(n) =
diphosphate + DNA(n+1).
-!- COFACTOR: Binds 2 magnesium ions per subunit (By similarity).
-!- SUBUNIT: Monomer (By similarity).
-!- SUBCELLULAR LOCATION: Cytoplasm (Probable).
-!- SIMILARITY: Belongs to the DNA polymerase type-Y family.
-!- SIMILARITY: Contains 1 umuC domain.
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Sequence length: 357
MRKIIHCDCD CFYAAIEMRD DPRLVGRPLA VGGRPERRGV VATCNYEARK FGIHSAMPMA
QAVKRCPDLL IVPPAMDKYR QVARQIFAIY QGYTPLVEPL SLDEAYLDVT DSPMLAGSGT
RIAEDIRRRV REEIGITVSA GVAPNKFIAK IASDWNKPDG LFVVRPEQVD AFVAALPVER
LFGVGKVTAA KLRRLGAQTC GDLRGWGADR LQQHFGSFGF RLHDLCRGID HRQVQPSQIR
KSVSVEETYA TDLRTLDDCQ RELTILVDQL AARVERARAG DMIHKTFVKL RFADFRGTTV
ECVYPQVALP VFNRLLAQGF ERRRMPVRLL GVGVRLHETD AHARQQALFA EGPPPGA