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Description:
UvrABC system protein B (Protein uvrB) (Excinuclease ABC subunit B).
Molecular weight: 78663
View which proteins in this organism that is involved with DNA Repair;
classified after biological processes (using data from the GOA project):
DNA repair( GO:0006281 ) base-excision repair( GO:0006284 ) nucleotide-excision repair (and GO:0045001, a synonym)( GO:0006289 )
Important dates:
20-JUN-2002, integrated into UniProtKB/Swiss-Prot.
01-MAR-2002, sequence version 1.
07-MAR-2006, entry version 24.
Phylogenetic order:
Bacteria Proteobacteria Betaproteobacteria Burkholderiales Burkholderiaceae Ralstonia.
To calculate the pI (Isoelectric point - the pH where a protein has a neutral charge),
go to this page and enter the protein ID (e.g 3MG_ECOLI): http://us.expasy.org/tools/pi_tool.html
Links to references in other databases for protein UVRB_RALSO:
| Database | Pointer | Add. info#1 | Add. info#2 |
| EMBL | AL646062 | CAD14713.1 | - |
| HSSP | Q56243 | 1C4O | |
| GenomeReviews | AL646052_GR | RSc1011.1 | |
| BioCyc | RSOL305:RSC1011-MONOMER | -.1 | |
| HAMAP | MF_00204 | - | 1. |
| InterPro | IPR001410 | DEAD. | |
| InterPro | IPR011545 | DEAD/DEAH_N. | |
| InterPro | IPR001650 | Helicase_C. | |
| InterPro | IPR006935 | ResIII. | |
| InterPro | IPR001943 | UvrB/C. | |
| InterPro | IPR004807 | UvrB_ABC. | |
| Pfam | PF00271 | Helicase_C | 1. |
| Pfam | PF04851 | ResIII | 1. |
| Pfam | PF02151 | UVR | 1. |
| SMART | SM00487 | DEXDc | 1. |
| SMART | SM00490 | HELICc | 1. |
| TIGRFAMs | TIGR00631 | uvrb | 1. |
| PROSITE | PS50151 | UVR | 1. |
Keywords:
ATP-binding; Complete proteome; DNA damage; DNA excision; DNA repair; Excision nuclease; Nucleotide-binding; SOS response.
References:
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=GMI1000;
RX MEDLINE=21681879; PubMed=11823852; DOI=10.1038/415497a;
RA Salanoubat M., Genin S., Artiguenave F., Gouzy J., Mangenot S.,
RA Arlat M., Billault A., Brottier P., Camus J.C., Cattolico L.,
RA Chandler M., Choisne N., Claudel-Renard C., Cunnac S., Demange N.,
RA Gaspin C., Lavie M., Moisan A., Robert C., Saurin W., Schiex T.,
RA Siguier P., Thebault P., Whalen M., Wincker P., Levy M.,
RA Weissenbach J., Boucher C.A.;
RT "Genome sequence of the plant pathogen Ralstonia solanacearum.";
RL Nature 415:497-502(2002).
Feature:
CHAIN 1 696 UvrABC system protein B.
/FTId=PRO_0000138419.
DOMAIN 647 682 UVR.
NP_BIND 58 65 ATP (Potential).
MOTIF 111 134 Beta-hairpin.
Comments:
-!- FUNCTION: The UvrABC repair system catalyzes the recognition and
processing of DNA lesions. A damage recognition complex composed
of 2 uvrA and 2 uvrB subunits scans DNA for abnormalities. Upon
binding of the uvrA(2)B(2) complex to a putative damaged site, the
DNA wraps around one uvrB monomer. DNA wrap is dependent on ATP
binding by uvrB and probably causes local melting of the DNA
helix, facilitating insertion of uvrB beta-hairpin between the DNA
strands. Then uvrB probes one DNA strand for the presence of a
lesion. If a lesion is found the uvrA subunits dissociate and the
uvrB-DNA preincision complex is formed. This complex is
subsequently bound by uvrC and the second uvrB is released. If no
lesion is found, the DNA wraps around the other uvrB subunit that
will check the other stand for damage (By similarity).
-!- SUBUNIT: Forms a heterotetramer with uvrA during the search for
lesions. Interacts with uvrC in an incision complex (By
similarity).
-!- SUBCELLULAR LOCATION: Cytoplasm (By similarity).
-!- DOMAIN: The beta-hairpin motif is involved in DNA binding (By
similarity).
-!- SIMILARITY: Belongs to the uvrB family.
-!- SIMILARITY: Contains 1 UVR domain.
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Sequence length: 696
MTDLSQVPSP YDESKFVTFD GSPFQLYQPY PPAGDQPEAI RQLVEGIGDG LSYQTLLGVT
GSGKTYTMAN VIAQAGRPAI VFAPNKTLAA QLYSEFREFF PRNAVEYFVS YYDYYQPEAY
VPQRDLFIEK DSSVNEHIEQ MRLSATKSLL ERRDVVIVAT VSAIYGIGNP TEYHQMILTL
RTGDKISQRD VIARLIAMQY TRNETDFQRG TFRVRGDTVD IFPAEHAEMA VRLELFDDEV
DSLQLFDPLT GRVRQKILRF TVYPSSHYVT PRETVLRAIG TIKAELRERL DFFYQENKLV
EAQRLEQRTR FDLEMLQELG FCKGIENYSR HLSGAQPGEP PPTLVDYLPS DALMFLDESH
VLIGQLNGMY NGDRARKETL SAYGFRLPSA LDNRPLKFAE FEGKMRQVVF VSATPADYEK
QRAGDEVVEQ VVRPTGLVDP IIHVRPATTQ VDDLLSEIHE RVKAGERVLV TTLTKRMAEQ
LTEFLSENGV KVRYLHSDID TVERVEIIRD LRLGTFDVLV GINLLREGLD IPEVSLVAIL
DADKEGFLRA ERSLIQTIGR AARNVNGTAI LYGDRITESM KKAIGETERR RAKQIAHNEA
HGITPRGVVK RIKDIIDGVY NVDDARAELK AAQDAAKYED MSEKQVGKEI KRLEKQMLDH
AKNLEFEKAA AVRDQLAKLK SQVFGASGED HIVPAA