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Description:
DNA polymerase IV (EC 2.7.7.7) (Pol IV).
Molecular weight: 39320
View which proteins in this organism that is involved with DNA Repair;
classified after biological processes (using data from the GOA project):
DNA repair( GO:0006281 )
Important dates:
31-OCT-2003, integrated into UniProtKB/Swiss-Prot.
01-JUN-2003, sequence version 1.
07-MAR-2006, entry version 25.
Phylogenetic order:
Bacteria Proteobacteria Gammaproteobacteria Pseudomonadales Pseudomonadaceae Pseudomonas.
To calculate the pI (Isoelectric point - the pH where a protein has a neutral charge),
go to this page and enter the protein ID (e.g 3MG_ECOLI): http://us.expasy.org/tools/pi_tool.html
Links to references in other databases for protein DPO4_PSESM:
| Database | Pointer | Add. info#1 | Add. info#2 |
| EMBL | AE016853 | AAO57449.1 | - |
| HSSP | P96022 | 1IM4 | |
| GenomeReviews | AE016853_GR | PSPTO3990.1 | |
| TIGR | PSPTO3990 | -. | |
| BioCyc | PSYR223283:PSPTO3990-MONOMER | -.1 | |
| HAMAP | MF_01113 | - | 1. |
| InterPro | IPR001126 | UMUC_like. | |
| Pfam | PF00817 | IMS | 1. |
| PROSITE | PS50173 | UMUC | 1. |
Keywords:
Complete proteome; DNA damage; DNA repair; DNA replication; DNA-binding; DNA-directed DNA polymerase; Magnesium; Metal-binding; Mutator protein; Nucleotidyltransferase; Transferase.
References:
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=DC3000;
RX MEDLINE=22834015; PubMed=12928499; DOI=10.1073/pnas.1731982100;
RA Buell C.R., Joardar V., Lindeberg M., Selengut J., Paulsen I.T.,
RA Gwinn M.L., Dodson R.J., DeBoy R.T., Durkin A.S., Kolonay J.F.,
RA Madupu R., Daugherty S.C., Brinkac L.M., Beanan M.J., Haft D.H.,
RA Nelson W.C., Davidsen T.M., Zafar N., Zhou L., Liu J., Yuan Q.,
RA Khouri H.M., Fedorova N.B., Tran B., Russell D., Berry K.J.,
RA Utterback T.R., Van Aken S.E., Feldblyum T.V., D'Ascenzo M.,
RA Deng W.-L., Ramos A.R., Alfano J.R., Cartinhour S., Chatterjee A.K.,
RA Delaney T.P., Lazarowitz S.G., Martin G.B., Schneider D.J., Tang X.,
RA Bender C.L., White O., Fraser C.M., Collmer A.;
RT "The complete genome sequence of the Arabidopsis and tomato pathogen
RT Pseudomonas syringae pv. tomato DC3000.";
RL Proc. Natl. Acad. Sci. U.S.A. 100:10181-10186(2003).
Feature:
CHAIN 1 353 DNA polymerase IV.
/FTId=PRO_0000173934.
DOMAIN 6 187 UmuC.
ACT_SITE 106 106 By similarity.
METAL 10 10 Magnesium (By similarity).
METAL 105 105 Magnesium (By similarity).
SITE 15 15 Substrate discrimination (By similarity).
Comments:
-!- FUNCTION: Poorly processive, error-prone DNA polymerase involved
in untargeted mutagenesis. Copies undamaged DNA at stalled
replication forks, which arise in vivo from mismatched or
misaligned primer ends. These misaligned primers can be extended
by polIV. Exhibits no 3'-5' exonuclease (proofreading) activity.
May be involved in translesional synthesis, in conjunction with
the beta clamp from polIII (By similarity).
-!- CATALYTIC ACTIVITY: Deoxynucleoside triphosphate + DNA(n) =
diphosphate + DNA(n+1).
-!- COFACTOR: Binds 2 magnesium ions per subunit (By similarity).
-!- SUBUNIT: Monomer (By similarity).
-!- SUBCELLULAR LOCATION: Cytoplasm (Probable).
-!- SIMILARITY: Belongs to the DNA polymerase type-Y family.
-!- SIMILARITY: Contains 1 umuC domain.
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Sequence length: 353
MTQRKIIHID CDCFYAAIEM RDEPELAGKP LAVGGSAERR GVIATCNYEA RAYGVRSAMS
SRHALKLCPD LTIVKPRMEA YKEASREIHS IFRDYTDLIE PLSLDEAFLD VSDTHHFSGS
ATRIAQDIRR RVSNQLHITV SAGVAPNKFL AKIASDWKKP NGLFVITPDQ VEDFVASLPV
TKLHGVGKVT ADKLGRLGIV DCADLRSRSK LALVREFGSF GERLWSLAHG IDDRPVQNDS
RRQSVSVENT YDTDLPDLAA CLEKLPALLE TLGTRMERME GQYRPGKPFV KVKFHDFTQT
TLEQSGAGRD LGSYEQLLTQ AFARGGKPVR LLGIGVRLHD LRDAHEQLEL FST