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Description:
Crossover junction endodeoxyribonuclease ruvC (EC 3.1.22.4) (Hollidayjunction nuclease ruvC) (Holliday juction resolvase ruvC).
Molecular weight: 18602
View which proteins in this organism that is involved with DNA Repair;
classified after biological processes (using data from the GOA project):
DNA repair( GO:0006281 )
Important dates:
24-OCT-2003, integrated into UniProtKB/Swiss-Prot.
01-JUN-2003, sequence version 1.
07-MAR-2006, entry version 21.
Phylogenetic order:
Bacteria Proteobacteria Gammaproteobacteria Pseudomonadales Pseudomonadaceae Pseudomonas.
To calculate the pI (Isoelectric point - the pH where a protein has a neutral charge),
go to this page and enter the protein ID (e.g 3MG_ECOLI): http://us.expasy.org/tools/pi_tool.html
Links to references in other databases for protein RUVC_PSESM:
| Database | Pointer | Add. info#1 | Add. info#2 |
| EMBL | AE016853 | AAO57438.1 | - |
| HSSP | P24239 | 1HJR | |
| GenomeReviews | AE016853_GR | PSPTO3979.1 | |
| TIGR | PSPTO3979 | -. | |
| BioCyc | PSYR223283:PSPTO3979-MONOMER | -.1 | |
| HAMAP | MF_00034 | - | 1. |
| InterPro | IPR012337 | RNaseH_fold. | |
| InterPro | IPR002176 | RuvC. | |
| Pfam | PF02075 | RuvC | 1. |
| PRINTS | PR00696 | RSOLVASERUVC. | |
| ProDom | PD008333 | RuvC | 1. |
| TIGRFAMs | TIGR00228 | ruvC | 1. |
| PROSITE | PS01321 | RUVC | 1. |
Keywords:
Complete proteome; DNA damage; DNA recombination; DNA repair; Hydrolase; Magnesium; Metal-binding; Nuclease.
References:
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=DC3000;
RX MEDLINE=22834015; PubMed=12928499; DOI=10.1073/pnas.1731982100;
RA Buell C.R., Joardar V., Lindeberg M., Selengut J., Paulsen I.T.,
RA Gwinn M.L., Dodson R.J., DeBoy R.T., Durkin A.S., Kolonay J.F.,
RA Madupu R., Daugherty S.C., Brinkac L.M., Beanan M.J., Haft D.H.,
RA Nelson W.C., Davidsen T.M., Zafar N., Zhou L., Liu J., Yuan Q.,
RA Khouri H.M., Fedorova N.B., Tran B., Russell D., Berry K.J.,
RA Utterback T.R., Van Aken S.E., Feldblyum T.V., D'Ascenzo M.,
RA Deng W.-L., Ramos A.R., Alfano J.R., Cartinhour S., Chatterjee A.K.,
RA Delaney T.P., Lazarowitz S.G., Martin G.B., Schneider D.J., Tang X.,
RA Bender C.L., White O., Fraser C.M., Collmer A.;
RT "The complete genome sequence of the Arabidopsis and tomato pathogen
RT Pseudomonas syringae pv. tomato DC3000.";
RL Proc. Natl. Acad. Sci. U.S.A. 100:10181-10186(2003).
Feature:
CHAIN 1 176 Crossover junction endodeoxyribonuclease
ruvC.
/FTId=PRO_0000183123.
METAL 8 8 Magnesium (By similarity).
METAL 69 69 Magnesium (By similarity).
METAL 141 141 Magnesium (By similarity).
METAL 144 144 Magnesium (By similarity).
Comments:
-!- FUNCTION: Nuclease that resolves Holliday junction intermediates
in genetic recombination. Cleaves the cruciform structure in
supercoiled DNA by nicking to strands with the same polarity at
sites symmetrically opposed at the junction in the homologous arms
and leaves a 5'terminal phosphate and a 3'terminal hydroxyl group
(By similarity).
-!- CATALYTIC ACTIVITY: Endonucleolytic cleavage at a junction such as
a reciprocal single-stranded crossover between two homologous DNA
duplexes (Holliday junction).
-!- COFACTOR: Binds 1 magnesium ion per subunit (By similarity).
-!- SIMILARITY: Belongs to the ruvC family.
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Sequence length: 176
MTLILGIDPG SRITGYGVVR DTGRGCVYVA SGCIRTGTGS GELHERLQIV YRGVREVIKT
YGPVTMGIEK VFMARNADSA LKLGQARGAA IVAGAEEALE IAEYTATQVK QAVAGTGGAN
KEQVMMMVMH LLKLTQKPQI DASDALAIAL CHAHTRSSLI PHGLGTARSR GGRLRL