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Description:
LexA repressor (EC 3.4.21.88).
Molecular weight: 25622
View which proteins in this organism that is involved with DNA Repair;
classified after biological processes (using data from the GOA project):
DNA repair( GO:0006281 )
Important dates:
24-MAY-2004, integrated into UniProtKB/Swiss-Prot.
24-MAY-2004, sequence version 1.
07-FEB-2006, entry version 14.
Phylogenetic order:
Bacteria Proteobacteria Alphaproteobacteria Rhizobiales Rhizobiaceae Agrobacterium.
To calculate the pI (Isoelectric point - the pH where a protein has a neutral charge),
go to this page and enter the protein ID (e.g 3MG_ECOLI): http://us.expasy.org/tools/pi_tool.html
Links to references in other databases for protein LEXA_AGRTU:
| Database | Pointer | Add. info#1 | Add. info#2 |
| EMBL | AF251129 | AAQ14270.1 | - |
| HAMAP | MF_00015 | - | 1. |
| InterPro | IPR006199 | LexA_DNA_bd. | |
| InterPro | IPR006200 | Pept_S24_LexA. | |
| InterPro | IPR006198 | Pept_S24_S26. | |
| InterPro | IPR006197 | Pept_S24_SOS. | |
| InterPro | IPR011991 | Wing_hlx_DNA_bd. | |
| Pfam | PF01726 | LexA_DNA_bind | 1. |
| Pfam | PF00717 | Peptidase_S24 | 1. |
| PRINTS | PR00726 | LEXASERPTASE. | |
| TIGRFAMs | TIGR00498 | lexA | 1. |
Keywords:
Autocatalytic cleavage; DNA damage; DNA repair; DNA replication; DNA-binding; Hydrolase; Repressor; SOS response; Transcription; Transcription regulation.
References:
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RA Campoy S., Tapias A., Mazon G., Barbe J.;
RT "The lexA gene of Agrobacterium tumefaciens.";
RL Submitted (MAR-2000) to the EMBL/GenBank/DDBJ databases.
Feature:
CHAIN 1 239 LexA repressor.
/FTId=PRO_0000169999.
DNA_BIND 27 47 H-T-H motif (By similarity).
ACT_SITE 159 159 Involved in auto-cleavage (By
similarity).
ACT_SITE 197 197 Involved in auto-cleavage (By
similarity).
SITE 124 125 Cleavage (auto-) (By similarity).
Comments:
-!- FUNCTION: Represses a number of genes involved in the response to
DNA damage (SOS response), including recA and lexA. In the
presence of single-stranded DNA, recA interacts with lexA causing
an autocatalytic cleavage which disrupts the DNA-binding part of
lexA, leading to derepression of the SOS regulon and eventually
DNA repair (By similarity).
-!- CATALYTIC ACTIVITY: Hydrolysis of Ala-|-Gly bond in repressor
lexA.
-!- SUBUNIT: Homodimer (By similarity).
-!- SIMILARITY: Belongs to the peptidase S24 family.
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Sequence length: 239
MLTRKQQELL LFIHGTNEGN PGVPPSFDEM KDALDLASKS GIHRLITALE ERGFIRRLPN
RARALEVIKL PEAYAGASQV RRGFSPTVIE GSLGKLASPP PAPKPAPPAE AASVAVPVMG
RIAAGVPISA IQNNMHDISV PVEMIGSGEH YALEIKGDSM IEAGILDGDT VIIRNGSTAS
PGDIVVALID DEEATLKRFR RKGASIALEA ANPAYETRIF GPDRVKIQGR LVGLIRRYH