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Description:
Apurinic endonuclease-redox protein (DNA-(apurinic or apyrimidinicsite) lyase) (EC 4.2.99.18).
Molecular weight: 60260
View which proteins in this organism that is involved with DNA Repair;
classified after biological processes (using data from the GOA project):
DNA repair( GO:0006281 )
Important dates:
01-NOV-1995, integrated into UniProtKB/Swiss-Prot.
30-MAY-2000, sequence version 2.
07-MAR-2006, entry version 50.
Phylogenetic order:
Eukaryota Viridiplantae Streptophyta Embryophyta Tracheophyta Spermatophyta Magnoliophyta eudicotyledons core eudicotyledons rosids eurosids II Brassicales Brassicaceae Arabidopsis.
To calculate the pI (Isoelectric point - the pH where a protein has a neutral charge),
go to this page and enter the protein ID (e.g 3MG_ECOLI): http://us.expasy.org/tools/pi_tool.html
Links to references in other databases for protein ARP_ARATH:
| Database | Pointer | Add. info#1 | Add. info#2 |
| EMBL | AC004625 | AAC23731.1 | - |
| EMBL | X76912 | CAA54234.1 | - |
| PIR | T02441 | T02441. | |
| HSSP | P27695 | 1E9N | |
| GenomeReviews | CT485783_GR | AT2G41460.1 | |
| TAIR | At2g41460 | -.1 | |
| InterPro | IPR000097 | APendonclse1. | |
| InterPro | IPR005135 | Exo_endo_phos. | |
| InterPro | IPR004808 | ExoIII_xth. | |
| InterPro | IPR003034 | SAP_DNA_bd. | |
| Pfam | PF03372 | Exo_endo_phos | 1. |
| Pfam | PF02037 | SAP | 1. |
| SMART | SM00513 | SAP | 1. |
| TIGRFAMs | TIGR00195 | exoDNase_III | 1. |
| TIGRFAMs | TIGR00633 | xth | 1. |
| PROSITE | PS00726 | AP_NUCLEASE_F1_1 | 1. |
| PROSITE | PS00727 | AP_NUCLEASE_F1_2 | 1. |
| PROSITE | PS00728 | AP_NUCLEASE_F1_3 | 1. |
| PROSITE | PS50800 | SAP | 1. |
Keywords:
DNA damage; DNA repair; Lyase; Magnesium; Metal-binding; Nuclear protein.
References:
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX MEDLINE=20083487; PubMed=10617197; DOI=10.1038/45471;
RA Lin X., Kaul S., Rounsley S.D., Shea T.P., Benito M.-I., Town C.D.,
RA Fujii C.Y., Mason T.M., Bowman C.L., Barnstead M.E., Feldblyum T.V.,
RA Buell C.R., Ketchum K.A., Lee J.J., Ronning C.M., Koo H.L.,
RA Moffat K.S., Cronin L.A., Shen M., Pai G., Van Aken S., Umayam L.,
RA Tallon L.J., Gill J.E., Adams M.D., Carrera A.J., Creasy T.H.,
RA Goodman H.M., Somerville C.R., Copenhaver G.P., Preuss D.,
RA Nierman W.C., White O., Eisen J.A., Salzberg S.L., Fraser C.M.,
RA Venter J.C.;
RT "Sequence and analysis of chromosome 2 of the plant Arabidopsis
RT thaliana.";
RL Nature 402:761-768(1999).
RN [2]
RP NUCLEOTIDE SEQUENCE OF 10-536.
RC STRAIN=cv. Columbia; TISSUE=Callus;
RX MEDLINE=94211851; PubMed=7512729;
RA Babiychuk E., Kushnir S., van Montagu M., Inze D.;
RT "The Arabidopsis thaliana apurinic endonuclease Arp reduces human
RT transcription factors Fos and Jun.";
RL Proc. Natl. Acad. Sci. U.S.A. 91:3299-3303(1994).
Feature:
CHAIN 1 536 Apurinic endonuclease-redox protein.
/FTId=PRO_0000200018.
DOMAIN 97 131 SAP.
REGION 1 278 Highly charged; increases the affinity of
ARP for DNA.
REGION 279 536 AP endonuclease.
ACT_SITE 527 527 Proton acceptor (By similarity).
METAL 313 313 Magnesium or manganese (By similarity).
Comments:
-!- FUNCTION: Repairs oxidative DNA damages, seems also to act as a
redox factor. Is multifunctional and may be involved both in DNA
repair and in the regulation of transcription.
-!- CATALYTIC ACTIVITY: The C-O-P bond 3' to the apurinic or
apyrimidinic site in DNA is broken by a beta-elimination reaction,
leaving a 3'-terminal unsaturated sugar and a product with a
terminal 5'-phosphate.
-!- SUBCELLULAR LOCATION: Nucleus.
-!- TISSUE SPECIFICITY: Expressed in the siliques, flowers, and stems.
A high level expression is seen in the leaves.
-!- SIMILARITY: Belongs to the DNA repair enzymes AP/exoA family.
-!- SIMILARITY: Contains 1 SAP domain.
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Sequence length: 536
MNNVLQFGLQ SSAIYVAKFL VVPLRSLRVG SSFVGVGVGT RSFNKRLMSN ATAFSINNSK
RKELKIPGAA IDQNCHQMGS DTDRDEMGTL QDDRKEIEAM TVQELRSTLR KLGVPVKGRK
QELISTLRLH MDSNLPDQKE TSSSTRSDSV TIKRKISNRE EPTEDECTNS EAYDIEHGEK
RVKQSTEKNL KAKVSAKAIA KEQKSLMRTG KQQIQSKEET SSTISSELLK TEEIISSPSQ
SEPWTVLAHK KPQKDWKAYN PKTMRPPPLP EGTKCVKVMT WNVNGLRGLL KFESFSALQL
AQRENFDILC LQETKLQVKD VEEIKKTLID GYDHSFWSCS VSKLGYSGTA IISRIKPLSV
RYGTGLSGHD TEGRIVTAEF DSFYLINTYV PNSGDGLKRL SYRIEEWDRT LSNHIKELEK
SKPVVLTGDL NCAHEEIDIF NPAGNKRSAG FTIEERQSFG ANLLDKGFVD TFRKQHPGVV
GYTYWGYRHG GRKTNKGWRL DYFLVSQSIA ANVHDSYILP DINGSDHCPI GLILKL