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Description:
Serine/threonine-protein kinase ATM (EC 2.7.1.37) (Ataxiatelangiectasia mutated homolog) (AtATM).
Molecular weight: 4351
View which proteins in this organism that is involved with DNA Repair;
classified after biological processes (using data from the GOA project):
DNA repair( GO:0006281 )
Important dates:
21-DEC-2004, integrated into UniProtKB/Swiss-Prot.
01-OCT-2000, sequence version 1.
07-MAR-2006, entry version 25.
Phylogenetic order:
Eukaryota Viridiplantae Streptophyta Embryophyta Tracheophyta Spermatophyta Magnoliophyta eudicotyledons core eudicotyledons rosids eurosids II Brassicales Brassicaceae Arabidopsis.
To calculate the pI (Isoelectric point - the pH where a protein has a neutral charge),
go to this page and enter the protein ID (e.g 3MG_ECOLI): http://us.expasy.org/tools/pi_tool.html
Links to references in other databases for protein ATM_ARATH:
| Database | Pointer | Add. info#1 | Add. info#2 |
| EMBL | AJ250248 | CAB86487.1 | - |
| EMBL | AL096856 | CAB92122.1 | - |
| PIR | T51174 | T51174. | |
| GenomeReviews | BA000014_GR | AT3G48190.1 | |
| GeneFarm | 4562 | -.1 | |
| TAIR | At3g48190 | -.1 | |
| InterPro | IPR011989 | ARM-like. | |
| InterPro | IPR003152 | FATC. | |
| InterPro | IPR000403 | PI3/4_kinase_cat. | |
| InterPro | IPR000313 | PWWP. | |
| Pfam | PF02260 | FATC | 1. |
| Pfam | PF00454 | PI3_PI4_kinase | 1. |
| Pfam | PF00855 | PWWP | 1. |
| SMART | SM00146 | PI3Kc | 1. |
| PROSITE | PS00915 | PI3_4_KINASE_1 | 1. |
| PROSITE | PS00916 | PI3_4_KINASE_2 | 1. |
| PROSITE | PS50290 | PI3_4_KINASE_3 | 1. |
| PROSITE | PS50812 | PWWP | 1. |
Keywords:
Cell cycle; Chromosomal protein; DNA damage; DNA repair; DNA-binding; Kinase; Meiosis; Nuclear protein; Serine/threonine-protein kinase; Transferase.
References:
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND TISSUE SPECIFICITY.
RC STRAIN=cv. Columbia;
RX MEDLINE=20200600; PubMed=10734187; DOI=10.1093/nar/28.8.1692;
RA Garcia V., Salanoubat M., Choisne N., Tissier A.;
RT "An ATM homologue from Arabidopsis thaliana: complete genomic
RT organisation and expression analysis.";
RL Nucleic Acids Res. 28:1692-1699(2000).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX MEDLINE=21016720; PubMed=11130713; DOI=10.1038/35048706;
RA Salanoubat M., Lemcke K., Rieger M., Ansorge W., Unseld M.,
RA Fartmann B., Valle G., Bloecker H., Perez-Alonso M., Obermaier B.,
RA Delseny M., Boutry M., Grivell L.A., Mache R., Puigdomenech P.,
RA De Simone V., Choisne N., Artiguenave F., Robert C., Brottier P.,
RA Wincker P., Cattolico L., Weissenbach J., Saurin W., Quetier F.,
RA Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Benes V.,
RA Wurmbach E., Drzonek H., Erfle H., Jordan N., Bangert S.,
RA Wiedelmann R., Kranz H., Voss H., Holland R., Brandt P., Nyakatura G.,
RA Vezzi A., D'Angelo M., Pallavicini A., Toppo S., Simionati B.,
RA Conrad A., Hornischer K., Kauer G., Loehnert T.-H., Nordsiek G.,
RA Reichelt J., Scharfe M., Schoen O., Bargues M., Terol J., Climent J.,
RA Navarro P., Collado C., Perez-Perez A., Ottenwaelder B., Duchemin D.,
RA Cooke R., Laudie M., Berger-Llauro C., Purnelle B., Masuy D.,
RA de Haan M., Maarse A.C., Alcaraz J.-P., Cottet A., Casacuberta E.,
RA Monfort A., Argiriou A., Flores M., Liguori R., Vitale D.,
RA Mannhaupt G., Haase D., Schoof H., Rudd S., Zaccaria P., Mewes H.-W.,
RA Mayer K.F.X., Kaul S., Town C.D., Koo H.L., Tallon L.J., Jenkins J.,
RA Rooney T., Rizzo M., Walts A., Utterback T., Fujii C.Y., Shea T.P.,
RA Creasy T.H., Haas B., Maiti R., Wu D., Peterson J., Van Aken S.,
RA Pai G., Militscher J., Sellers P., Gill J.E., Feldblyum T.V.,
RA Preuss D., Lin X., Nierman W.C., Salzberg S.L., White O., Venter J.C.,
RA Fraser C.M., Kaneko T., Nakamura Y., Sato S., Kato T., Asamizu E.,
RA Sasamoto S., Kimura T., Idesawa K., Kawashima K., Kishida Y.,
RA Kiyokawa C., Kohara M., Matsumoto M., Matsuno A., Muraki A.,
RA Nakayama S., Nakazaki N., Shinpo S., Takeuchi C., Wada T.,
RA Watanabe A., Yamada M., Yasuda M., Tabata S.;
RT "Sequence and analysis of chromosome 3 of the plant Arabidopsis
RT thaliana.";
RL Nature 408:820-822(2000).
RN [3]
RP FUNCTION.
RX MEDLINE=22397959; PubMed=12509526; DOI=10.1105/tpc.006577;
RA Garcia V., Bruchet H., Camescasse D., Granier F., Bouchez D.,
RA Tissier A.;
RT "AtATM is essential for meiosis and the somatic response to DNA damage
RT in plants.";
RL Plant Cell 15:119-132(2003).
RN [4]
RP FUNCTION.
RX PubMed=16166376; DOI=10.1101/gad.1333805;
RA Vespa L., Couvillion M., Spangler E., Shippen D.E.;
RT "ATM and ATR make distinct contributions to chromosome end protection
RT and the maintenance of telomeric DNA in Arabidopsis.";
RL Genes Dev. 19:2111-2115(2005).
Feature:
CHAIN 1 3856 Serine/threonine-protein kinase ATM.
/FTId=PRO_0000088843.
DOMAIN 108 162 PWWP.
DOMAIN 2727 3393 FAT.
DOMAIN 3525 3773 PI3K/PI4K.
DOMAIN 3824 3856 FATC.
MOTIF 3233 3249 Bipartite nuclear localization signal
(Potential).
CONFLICT 3640 3640 P -> S (in Ref. 1).
Comments:
-!- FUNCTION: Plays a central role in the perception and response to
both stress-induced damage in somatic cells and developmentally
programmed DNA damage during meiosis. Involved in transcriptional
regulation of RAD51, AtPARP1, AtGR1, and AtLIG4 in response to DNA
double strand breaks (DSBs). Plays a dual role by activating the
DNA damage response at dysfunctional telomeres and yet preventing
this activation at functional telomeres. Not required for telomere
length homeostasis.
-!- CATALYTIC ACTIVITY: ATP + a protein = ADP + a phosphoprotein.
-!- SUBCELLULAR LOCATION: Nucleus (Probable).
-!- TISSUE SPECIFICITY: Ubiquitously expressed at low levels with
slightly higher levels in flower buds.
-!- SIMILARITY: Belongs to the PI3/PI4-kinase family.
-!- SIMILARITY: Contains 1 FAT domain.
-!- SIMILARITY: Contains 1 FATC domain.
-!- SIMILARITY: Contains 1 PI3K/PI4K domain.
-!- SIMILARITY: Contains 1 PWWP domain.
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Sequence length: 3856
MKLQNPDKKT LREGFSQESS VVALDSGVLA MSGLKCDGKF PVKDVLMEEG GDKVRKIQVS
GGNISLVVDF SGARTSSNNF FESNASCVNE NLVKGNGYRE DETQEFLVGN LVWVMTKYKK
WWPGEVVDFK ADAKESFMVR SIGQSHLVSW FASSKLKPFK ESFEQVLNQR NDNGFFDALQ
KAMSLLSNSL KLDMTCSCIA DGNGIVSAQN ITTRKNKPLI LREFSVDRLE PKEFVTQLKN
IAKCVLNAGV LESTVMQSQL SAFYTLFGHK QIPMAQLHEN EGRKSFTAKM SDSKFIGSPS
ICAGNSRKRF RKEWFRKFVS EVDNVSARDD LVNVPPSDLI SKLKLLAVGY NCSEETENIG
LFEWFFSKFR ISVYHDENAY KMQLANMAGF KDLMLATNAN RGTVQKTLKS KKIGKSKMEP
LNGVSVADTE QKTFELQISK KSNIESLNGV SVADTEQKTF ELQILEKSNI ESLNGVSTPN
IDHEASKSNN SGKTKINHII GHSNFPSSVA KVQLAKDFQD KLLVQAPDRK AMTADTLSRP
AAILVPDLNS GGNALGTAEF DHMQRPETLI QHNVCPQEEK TPRSTILNFQ VTAHQGVSGT
QFVSSQPTSY KHFTSADLFT YSGKKKRGRK RKNAEELPIV AHASATTGIP DLNGTNTEPT
LVLPQVEPTQ RRRRRKKEES PNGLTRGITI LFLKFSSQVS MPSRDDLTST FSAFGPLDSS
ETHVSEEFSG AQVAFVSSAD AIEAVKSLEK ANPFGETLVN FRLQQKLITV QRNIAPRMPV
ISHVSPVPKP NNIPTSMDAM RQNLLMMTAM LEKSGDSLSR ETKAKLKSEI TGLLEKDGVK
LLNTWLEGER SITFCRFLSQ NTAKLKLDEI PNAETWPFLV KLLLQCVSME VSGSKRRMPK
PTFAKTLRVV VQRTEETKFP GVQFPLLSMA KTLFTHVHDI LSNTPSFQSE YGTILRHLLE
IKEYRFQMRK RTYSSLVLLY MERAETGFCE KNSGQHSQKE EAFRYILTLQ SLLENSPGDF
PDDLREEIVN GLIHIFSSVR DEGKLSRKLI ECVNTFLLKD GPNLGSLSLE IHNAVEQFVF
RCWLTTHDKN LKEILVSYGR LQLNLTRDSS ESSSLVEQLL DVVTRELDLG SSSSSASWGD
TTKDEKLGAL SSYQNSLVEL AAHVFYRACV NTSRPSLSEK RARRQHIAMR MVDALTEGKW
LWCAAFGCLV RNYCARINMD LLIYWFEAIC TNFQRLLEDA SMRRSYDGLL WTLRSLQGLS
SGLSLPDITM DISKSSASSS ELDRGWQSIW SSLIHGLATF SSMSVIVDAV LVLLGSIISS
NHITVKILPQ EVWDHQLFRH IPSEPALYFI ACYFSRMGCQ GNLQDDLHLR RNLLRAVCAP
LSWKVRLTLD ERMVQLLPAA AFSLCAGFKV SLPLPKEHLP TPSQWDVCEQ IDDVDRERNF
GLFECSVEAL TRICSNSSKI SGCQVPDVVQ LPLVLRDPLL HDMDIYFLSI IPEVKEKGPL
SDIFMGCALL CHFMHGSYIT RKGKGSSSFF LKACQYLLEG LDHAVESVSK SLNDLQRRGS
LGFGSDFNEK GSIIVSLRSF TQSPVFSNRR DQNLLGASYD FVIHSLENLL RSFAKVYEEY
TEHAWNTHSD TVPSKSLAPD SPEVGRIVDM DLDLAEDTKE RDIIAAGGKA VPGLPVSMGN
WKLGMVSLIS CFSPVLQFPT WDVLYNLLEK ESDPKVLENI LYHLCKLSCL TSIPKVDDLV
IFLDGMLSTQ VKMKRNCLNI VTALHVLLHT LSSSRRDSSG VEKNCGLSLK EAESFQVFVQ
LGAMVNKVSE FGLLGWFGRV KLINCICDLV LLNPQTGQTM IERLLLMLSD SDYRVRFVLA
RQIGILFQTW DGHEALFQDI CSSFGIKLVT SSKEKLVTAK DVLAVGPQPR QKMETVIITL
MHLAYHSENI ELQAVFMMCA VSAKDPCQRE LIIAALDNLS AQLHYPSRFK YLEELLGPIL
FHWIASGVSL AGLIETSQLF IPNAEPKYFI HFCSHWLLPA LLLHEDHTNL DWVAKMAGQP
VVVLVKENFV PIFSICMGLH CSKTSECDKG AMVLQNSILY VGETSENERD KLIKQNMVSI
VSFILSCASS SPEPPVPTFS RDTISLAVQT VVDGFLENTD YPKNAAITDR INIFRPDRVF
MFITEMHYRM SAACHHRHTR HHLAALEELT ILLGHRALVP SSLNYIFNLV GQFIGYPSLQ
DQCCSIASCL LDLFKSNPAK EIVSVLGDQL QFLVSKLVTC CIDAEADTKI SGAKSSQLVN
LLHKLVVSSD SSLNEDIRDL EPLPDLKYFQ VIRESHIRIC EAYSPRNHLL KVEHSTFLIY
IFLEILSLSN FLFLSCSTIQ QCSRRSNYLP PRFLSRSLQA LHNKLIASEV SQEDTNGETA
ETFWQSDDEI VNAVWTLVRV SASDEADSMR LLVSDFLSRI GIRDPHTVVF HLPGNLVSMH
GLQGFGHNTG SKVRSLTENG ISDETLITLL NFLKKYLLDD SVKIIDVTSQ TLRGILSTER
GQQALSSFDS CERALIEVHG RGVNLDIVEK ILLDSQKQFK AEKFSLETPE VWSTDNKNFD
RWICQLVYCM IALCEDVPIR LCQNIALLKA EISELLFPSV VVSLAGRIGM DINLHDLITS
QVKEHIFTDS NKLTKSKQVM LNTLNELRMC YVLERSIFSG QTKREKNSRS CSTAAKIRDV
ESGSNGMAAS ITTNWEKVYW LSIDYLVVAG SAVVCGAYLT ASMYVEYWCE EKFGNLSLGD
PDFSYHDKLP DHVEILVSAI TRINEPDSLY GVIHSNKLSA QIITFEHEGN WTRALEYYDL
QARSQKMVVP SSLSENLEVE QFQPTTSARH SVFGEGEVQR QPFKGLIRSL QQTGCMHVLD
LYCRGLTSRE GCFQYDPEFI ELQYEAAWRA GKWDFSLLYP QTHCQPLQHA KNNNYHESLH
CCLRALQEGD YDGFYGKLKD TKKELVLSIS RASEESTEFI YSTVVKLQIL HHLGLVWDLR
WTTSSHQSVH GYLVKQMACV DPVIPTMDQL SWLNKDWNSI ITQTQLHMTL LEPFIAFRRV
LLQILGCEKC TMQHLLQSAS LLRKGTRFSH AAASLHEFKF LCARSNGQQP VPDWLGKLEE
AKLLHAQGRH EVSISLANYI LHNYQLKEEA SDIYRVIGKW LAETRSSNSR TILEKYLRPA
VSLAEEQSSK ICKRLVDRQS QTWFHLAHYA DALFKSYEER LSSSEWQAAL RLRKHKTKEL
EVFIKRFKSS KKAEQSDYSL KIQDLQKQLT MDKEEAEKLQ VDRDNFLKLA LEGYKRCLEI
GDKYDVRVVF RQVSMWFSLA SQKNVIDNML STIKEVQSYK FIPLVYQIAS RLGSSKDESG
SNSFQSALVS LIRKMAIDHP YHTILQLLAL ANGDRIKDNQ RSRNSFVVDM DKKLAAEHLL
QDVSHYHGPM IRQMKQLVDI YIKLAELETR REDTNRKVAL PREIRSVKQL ELVPVVTATI
PVDRSCQYNE GSFPFFRGLS DSVTVMNGIN APKVVECFGS DGQKYKQLAK SGNDDLRQDA
VMEQFFGLVN TFLHNNRDTW KRRLAVRTYK VIPFTPSAGV LEWVDGTIPL GDYLIGSSRS
EGAHGRYGIG NWKYPKCREH MSSAKDKRKA FVDVCTNFRP VMHYFFLEKF LQPADWFVKR
LAYTRSVAAS SMVGYIVGLG DRHAMNILID QATAEVVHID LGVAFEQGLM LKTPERVPFR
LTRDIIDGMG ITGVEGVFRR CCEETLSVMR TNKEALLTIV EVFIHDPLYK WALSPLKALQ
RQKETEDYDG MNLEGLQEEF EGNKDATRAL MRVKQKLDGY EGGEMRSIHG QAQQLIQDAI
DTDRLSHMFP GWGAWM