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Description:
Cryptochrome 2 apoprotein (Blue light photoreceptor).
Molecular weight: 69457
View which proteins in this organism that is involved with DNA Repair;
classified after biological processes (using data from the GOA project):
DNA repair( GO:0006281 )
Important dates:
15-DEC-1998, integrated into UniProtKB/Swiss-Prot.
15-DEC-1998, sequence version 2.
07-MAR-2006, entry version 52.
Phylogenetic order:
Eukaryota Viridiplantae Streptophyta Embryophyta Tracheophyta Spermatophyta Magnoliophyta eudicotyledons core eudicotyledons rosids eurosids II Brassicales Brassicaceae Arabidopsis.
To calculate the pI (Isoelectric point - the pH where a protein has a neutral charge),
go to this page and enter the protein ID (e.g 3MG_ECOLI): http://us.expasy.org/tools/pi_tool.html
Links to references in other databases for protein CRY2_ARATH:
| Database | Pointer | Add. info#1 | Add. info#2 |
| EMBL | U62549 | AAB04996.1 | - |
| EMBL | U62550 | AAB04997.1 | - |
| EMBL | X99061 | CAA67508.1 | - |
| EMBL | U43397 | AAD09837.1 | - |
| EMBL | AY057440 | AAL16377.1 | - |
| EMBL | AY057441 | AAL16378.1 | - |
| EMBL | AY057442 | AAL16379.1 | - |
| EMBL | AC000104 | AAB70435.1 | - |
| EMBL | BT008576 | AAP40403.1 | - |
| EMBL | BT008648 | AAP40463.1 | - |
| HSSP | P37250 | 1IQR | |
| IntAct | Q96524 | -.1 | |
| GenomeReviews | CT485782_GR | AT1G04400.1 | |
| TAIR | At1g04400 | -.1 | |
| GO | GO:0005515 | F:protein binding | IPI. |
| InterPro | IPR002081 | DNA_photolyase_1. | |
| InterPro | IPR006050 | DNA_photolyase_N. | |
| InterPro | IPR006051 | FAD_bd_N. | |
| InterPro | IPR005101 | Photolyse_FAD_bd. | |
| Pfam | PF00875 | DNA_photolyase | 1. |
| Pfam | PF03441 | FAD_binding_7 | 1. |
| PRINTS | PR00147 | DNAPHOTLYASE. | |
| ProDom | PD004390 | FAD_binding_N | 1. |
| PROSITE | PS00394 | DNA_PHOTOLYASES_1_1 | 1. |
| PROSITE | PS00691 | DNA_PHOTOLYASES_1_2 | 1. |
Keywords:
ATP-binding; Chromophore; FAD; Flavoprotein; Nucleotide-binding; Photoreceptor protein; Receptor; Sensory transduction.
References:
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA].
RC STRAIN=cv. Columbia;
RX MEDLINE=97156931; PubMed=9003312; DOI=10.1007/s004380050321;
RA Hoffman P.D., Batschauer A., Hays J.B.;
RT "PHH1, a novel gene from Arabidopsis thaliana that encodes a protein
RT similar to plant blue-light photoreceptors and microbial
RT photolyases.";
RL Mol. Gen. Genet. 253:259-265(1996).
RN [2]
RP NUCLEOTIDE SEQUENCE [MRNA].
RC STRAIN=cv. Columbia;
RA Lin C., Ahmad M., Chan J., Cashmore A.R.;
RT "CRY2: a second member of the Arabidopsis cryptochrome gene family.";
RL (er) Plant Gene Register PGR96-001.
RN [3]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA].
RX MEDLINE=21583762; PubMed=11726930; DOI=10.1038/ng767;
RA El-Din El-Assal S., Alonso-Blanco C., Peeters A.J.M., Raz V.,
RA Koornneef M.;
RT "A QTL for flowering time in Arabidopsis reveals a novel allele of
RT CRY2.";
RL Nat. Genet. 29:435-440(2001).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX MEDLINE=21016719; PubMed=11130712; DOI=10.1038/35048500;
RA Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S.,
RA White O., Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y.,
RA Buehler E., Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W.,
RA Chung M.K., Conn L., Conway A.B., Conway A.R., Creasy T.H., Dewar K.,
RA Dunn P., Etgu P., Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y.,
RA Gill J.E., Goldsmith A.D., Haas B., Hansen N.F., Hughes B., Huizar L.,
RA Hunter J.L., Jenkins J., Johnson-Hopson C., Khan S., Khaykin E.,
RA Kim C.J., Koo H.L., Kremenetskaia I., Kurtz D.B., Kwan A., Lam B.,
RA Langin-Hooper S., Lee A., Lee J.M., Lenz C.A., Li J.H., Li Y.-P.,
RA Lin X., Liu S.X., Liu Z.A., Luros J.S., Maiti R., Marziali A.,
RA Militscher J., Miranda M., Nguyen M., Nierman W.C., Osborne B.I.,
RA Pai G., Peterson J., Pham P.K., Rizzo M., Rooney T., Rowley D.,
RA Sakano H., Salzberg S.L., Schwartz J.R., Shinn P., Southwick A.M.,
RA Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D.,
RA Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M.,
RA Wu D., Yu G., Fraser C.M., Venter J.C., Davis R.W.;
RT "Sequence and analysis of chromosome 1 of the plant Arabidopsis
RT thaliana.";
RL Nature 408:816-820(2000).
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RX MEDLINE=22954850; PubMed=14593172; DOI=10.1126/science.1088305;
RA Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J.,
RA Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F.,
RA Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G.,
RA Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J.,
RA Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y.,
RA Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P.,
RA Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F.,
RA Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M.,
RA Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J.,
RA Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T.,
RA Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y.,
RA Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.;
RT "Empirical analysis of transcriptional activity in the Arabidopsis
RT genome.";
RL Science 302:842-846(2003).
Feature:
CHAIN 1 612 Cryptochrome 2 apoprotein.
/FTId=PRO_0000085122.
NP_BIND 244 248 FAD (By similarity).
BINDING 232 232 FAD (By similarity).
BINDING 357 357 ATP (By similarity).
BINDING 387 387 FAD; via carbonyl oxygen (By similarity).
BINDING 389 389 FAD; via amide nitrogen (By similarity).
BINDING 406 406 ATP (By similarity).
CONFLICT 78 78 K -> Q (in Ref. 1; AAB04996/AAB04997).
CONFLICT 95 95 A -> P (in Ref. 1; AAB04996/AAB04997).
CONFLICT 127 127 Q -> S (in Ref. 3; AAL16379).
CONFLICT 188 188 S -> L (in Ref. 3; AAL16379).
CONFLICT 366 366 A -> G (in Ref. 1; AAB04996).
CONFLICT 367 367 V -> M (in Ref. 3; AAL16377/AAL16378).
CONFLICT 476 476 T -> I (in Ref. 3; AAL16377/AAL16378).
CONFLICT 534 534 A -> V (in Ref. 1; AAB04996/AAB04997).
CONFLICT 590 590 K -> E (in Ref. 1).
CONFLICT 612 612 K -> Q (in Ref. 1).
Comments:
-!- FUNCTION: May mediate blue light-induced gene expression in
addition to a role in blue light-dependent inhibition of stem
growth.
-!- COFACTOR: Binds 1 FAD per subunit (By similarity).
-!- COFACTOR: Binds 1 8-hydroxy-5-deazaflavin (F420) noncovalently per
subunit (By similarity).
-!- INTERACTION:
P14713:PHYB; NbExp=2; IntAct=EBI-531555, EBI-300727;
-!- SIMILARITY: Belongs to the DNA photolyase class-1 family.
-!- CAUTION: Was originally (Ref.1) thought to be a DNA photolyase.
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Sequence length: 612
MKMDKKTIVW FRRDLRIEDN PALAAAAHEG SVFPVFIWCP EEEGQFYPGR ASRWWMKQSL
AHLSQSLKAL GSDLTLIKTH NTISAILDCI RVTGATKVVF NHLYDPVSLV RDHTVKEKLV
ERGISVQSYN GDLLYEPWEI YCEKGKPFTS FNSYWKKCLD MSIESVMLPP PWRLMPITAA
AEAIWACSIE ELGLENEAEK PSNALLTRAW SPGWSNADKL LNEFIEKQLI DYAKNSKKVV
GNSTSLLSPY LHFGEISVRH VFQCARMKQI IWARDKNSEG EESADLFLRG IGLREYSRYI
CFNFPFTHEQ SLLSHLRFFP WDADVDKFKA WRQGRTGYPL VDAGMRELWA TGWMHNRIRV
IVSSFAVKFL LLPWKWGMKY FWDTLLDADL ECDILGWQYI SGSIPDGHEL DRLDNPALQG
AKYDPEGEYI RQWLPELARL PTEWIHHPWD APLTVLKASG VELGTNYAKP IVDIDTAREL
LAKAISRTRE AQIMIGAAPD EIVADSFEAL GANTIKEPGL CPSVSSNDQQ VPSAVRYNGS
KRVKPEEEEE RDMKKSRGFD ERELFSTAES SSSSSVFFVS QSCSLASEGK NLEGIQDSSD
QITTSLGKNG CK