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Description:
DNA ligase 4 (EC 6.5.1.1) (DNA ligase IV) (Polydeoxyribonucleotidesynthase [ATP] 4).
Molecular weight: 1378
View which proteins in this organism that is involved with DNA Repair;
classified after biological processes (using data from the GOA project):
DNA repair( GO:0006281 )
Important dates:
24-JAN-2006, integrated into UniProtKB/Swiss-Prot.
01-OCT-2000, sequence version 1.
07-MAR-2006, entry version 21.
Phylogenetic order:
Eukaryota Viridiplantae Streptophyta Embryophyta Tracheophyta Spermatophyta Magnoliophyta eudicotyledons core eudicotyledons rosids eurosids II Brassicales Brassicaceae Arabidopsis.
To calculate the pI (Isoelectric point - the pH where a protein has a neutral charge),
go to this page and enter the protein ID (e.g 3MG_ECOLI): http://us.expasy.org/tools/pi_tool.html
Links to references in other databases for protein DNL4_ARATH:
| Database | Pointer | Add. info#1 | Add. info#2 |
| EMBL | AF233527 | AAF91284.1 | - |
| EMBL | AB023042 | BAA97366.1 | ALT_SEQ |
| GenomeReviews | BA000015_GR | AT5G57160.1 | |
| TAIR | At5g57160 | -.1 | |
| InterPro | IPR001357 | BRCT. | |
| InterPro | IPR000977 | DNA_ligase. | |
| InterPro | IPR012309 | DNA_ligase_A_C. | |
| InterPro | IPR012310 | DNA_ligase_A_M. | |
| InterPro | IPR012308 | DNA_ligase_A_N. | |
| PANTHER | PTHR10459 | DNA_ligase.1 | 1. |
| Pfam | PF00533 | BRCT | 2. |
| Pfam | PF04679 | DNA_ligase_A_C | 1. |
| Pfam | PF01068 | DNA_ligase_A_M | 1. |
| Pfam | PF04675 | DNA_ligase_A_N | 1. |
| SMART | SM00292 | BRCT | 1. |
| TIGRFAMs | TIGR00574 | dnl1 | 1. |
| PROSITE | PS50172 | BRCT | 2. |
| PROSITE | PS00697 | DNA_LIGASE_A1 | 1. |
| PROSITE | PS00333 | DNA_LIGASE_A2 | 1. |
| PROSITE | PS50160 | DNA_LIGASE_A3 | 1. |
Keywords:
ATP-binding; DNA damage; DNA recombination; DNA repair; DNA replication; Ligase; Nuclear protein; Nucleotide-binding; Repeat.
References:
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA], INDUCTION, TISSUE SPECIFICITY, AND
RP INTERACTION WITH XRCC4.
RX MEDLINE=20485436; PubMed=11029705;
RX DOI=10.1046/j.1365-313x.2000.00856.x;
RA West C.E., Waterworth W.M., Jiang Q., Bray C.M.;
RT "Arabidopsis DNA ligase IV is induced by gamma-irradiation and
RT interacts with an Arabidopsis homologue of the double strand break
RT repair protein XRCC4.";
RL Plant J. 24:67-78(2000).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX MEDLINE=20181125; PubMed=10718197; DOI=10.1093/dnares/7.1.31;
RA Sato S., Nakamura Y., Kaneko T., Katoh T., Asamizu E., Kotani H.,
RA Tabata S.;
RT "Structural analysis of Arabidopsis thaliana chromosome 5. X. Sequence
RT features of the regions of 3,076,755 bp covered by sixty P1 and TAC
RT clones.";
RL DNA Res. 7:31-63(2000).
RN [3]
RP FUNCTION.
RX PubMed=12853643; DOI=10.1093/nar/gkg458;
RA van Attikum H., Bundock P., Overmeer R.M., Lee L.-Y., Gelvin S.B.,
RA Hooykaas P.J.J.;
RT "The Arabidopsis AtLIG4 gene is required for the repair of DNA damage,
RT but not for the integration of Agrobacterium T-DNA.";
RL Nucleic Acids Res. 31:4247-4255(2003).
RN [4]
RP INDUCTION.
RX MEDLINE=22397959; PubMed=12509526; DOI=10.1105/tpc.006577;
RA Garcia V., Bruchet H., Camescasse D., Granier F., Bouchez D.,
RA Tissier A.;
RT "AtATM is essential for meiosis and the somatic response to DNA damage
RT in plants.";
RL Plant Cell 15:119-132(2003).
RN [5]
RP FUNCTION.
RX PubMed=12753583; DOI=10.1046/j.1365-313X.2003.01738.x;
RA Friesner J., Britt A.B.;
RT "Ku80- and DNA ligase IV-deficient plants are sensitive to ionizing
RT radiation and defective in T-DNA integration.";
RL Plant J. 34:427-440(2003).
Feature:
CHAIN 1 1219 DNA ligase 4.
/FTId=PRO_0000059580.
DOMAIN 651 739 BRCT 1.
DOMAIN 807 909 BRCT 2.
ACT_SITE 253 253 N6-AMP-lysine intermediate (By
similarity).
Comments:
-!- FUNCTION: Efficiently joins single-strand breaks in a double-
stranded polydeoxynucleotide in an ATP-dependent reaction.
Involved in DNA nonhomologous end joining (NHEJ) required for
double-strand break repair. May be involved for T-DNA integration
even if not absolutely required. Seems to be dispensable under
normal growth conditions.
-!- CATALYTIC ACTIVITY: ATP + (deoxyribonucleotide)(n) +
(deoxyribonucleotide)(m) = AMP + diphosphate +
(deoxyribonucleotide)(n+m).
-!- SUBUNIT: Interacts with XRCC4 via its tandem BRCT domains.
-!- SUBCELLULAR LOCATION: Nucleus (By similarity).
-!- TISSUE SPECIFICITY: Widely expressed, with higher levels in young
flowers and roots.
-!- INDUCTION: Induced by gamma radiation and by white light, but not
by UV-B. Regulated by ATM in response to DNA double strand breaks
(DSBs).
-!- SIMILARITY: Belongs to the ATP-dependent DNA ligase family.
-!- SIMILARITY: Contains 2 BRCT domains.
-!- CAUTION: Ref.2 sequence differs from that shown due to erroneous
gene model prediction.
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Sequence length: 1219
MTEEIKFSVL VSLFNWIQKS KTSSQKRSKF RKFLDTYCKP SDYFVAVRLI IPSLDRERGS
YGLKESVLAT CLIDALGISR DAPDAVRLLN WRKGGTAKAG ANAGNFSLIA AEVLQRRQGM
ASGGLTIKEL NDLLDRLASS ENRAEKTLVL STLIQKTNAQ EMKWVIRIIL KDLKLGMSEK
SIFQEFHPDA EDLFNVTCDL KLVCEKLRDR HQRHKRQDIE VGKAVRPQLA MRIGDVNAAW
KKLHGKDVVA ECKFDGDRIQ IHKNGTDIHY FSRNFLDHSE YAHAMSDLIV QNILVDKCIL
DGEMLVWDTS LNRFAEFGSN QEIAKAAREG LDSHKQLCYV AFDVLYVGDT SVIHQSLKER
HELLKKVVKP LKGRLEVLVP EGGLNVHRPS GEPSWSIVVH AAADVERFFK ETVENRDEGI
VLKDLESKWE PGDRSGKWMK LKPEYIRAGA DLDVLIIGGY YGSGRRGGEV AQFLVALADR
AEANVYPRRF MSFCRVGTGL SDDELNTVVS KLKPYFRKNE HPKKAPPSFY QVTNHSKERP
DVWIDSPEKS IILSITSDIR TIRSEVFVAP YSLRFPRIDK VRYDKPWHEC LDVQAFVELV
NSSNGTTQKQ KESESTQDNP KVNKSSKRGE KKNVSLVPSQ FIQTDVSDIK GKTSIFSNMI
FYFVNVPRSH SLETFHKMVV ENGGKFSMNL NNSVTHCIAA ESSGIKYQAA KRQRDVIHFS
WVLDCCSRNK MLPLLPKYFL HLTDASRTKL QDDIDEFSDS YYWDLDLEGL KQVLSNAKQS
EDSKSIDYYK KKLCPEKRWS CLLSCCVYFY PYSQTLSTEE EALLGIMAKR LMLEVLMAGG
KVSNNLAHAS HLVVLAMAEE PLDFTLVSKS FSEMEKRLLL KKRLHVVSSH WLEESLQREE
KLCEDVYTLR PKYMEESDTE ESDKSEHDTT EVASQGSAQT KEPASSKIAI TSSRGRSNTR
AVKRGRSSTN SLQRVQRRRG KQPSKISGDE TEESDASEEK VSTRLSDIAE ETDSFGEAQR
NSSRGKCAKR GKSRVGQTQR VQRSRRGKKA AKIGGDESDE NDELDGNNNV SADAEEGNAA
GRSVENEETR EPDIAKYTES QQRDNTVAVE EALQDSRNAK TEMDMKEKLQ IHEDPLQAML
MKMFPIPSQK TTETSNRTTG EYRKANVSGE CESSEKRKLD AETDNTSVNA GAESDVVPPL
VKKKKVSYRD VAGELLKDW