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Protein data for UVH1_ARATH:

Description:
DNA repair endonuclease UVH1 (EC 3.1.-.-) (Ultraviolet hypersensitive1) (AtRAD1) (DNA excision repair protein XP-F homolog).

Molecular weight: 10763

View which proteins in this organism that is involved with DNA Repair;
classified after biological processes (using data from the GOA project):
DNA repair( GO:0006281 ) base-excision repair( GO:0006284 )


Important dates:
23-NOV-2004, integrated into UniProtKB/Swiss-Prot.
23-NOV-2004, sequence version 2.
07-MAR-2006, entry version 24.

Phylogenetic order:
Eukaryota Viridiplantae Streptophyta Embryophyta Tracheophyta Spermatophyta Magnoliophyta eudicotyledons core eudicotyledons rosids eurosids II Brassicales Brassicaceae Arabidopsis.

To calculate the pI (Isoelectric point - the pH where a protein has a neutral charge),
go to this page and enter the protein ID (e.g 3MG_ECOLI): http://us.expasy.org/tools/pi_tool.html

Links to references in other databases for protein UVH1_ARATH:

DatabasePointerAdd. info#1Add. info#2
EMBLAF089003AAG42948.1-
EMBLAF160500AAF01274.1-
EMBLAF277377AAF81910.1-
EMBLAB010072BAB09717.1-
EMBLAY140076AAM98217.1-
EMBLAF191494AAF14584.1-
HSSPQ8TZH81J24
GenomeReviewsBA000015_GRAT5G41150.1
GeneFarm4434-.1
TAIRAt5g41150-.1
InterProIPR006166ERCC4.
PfamPF02732ERCC41.

General information about the databases mentioned above

Keywords:
Alternative splicing; DNA damage; DNA excision; DNA recombination; DNA repair; DNA-binding; Endonuclease; Hydrolase; Nuclear protein; Nuclease.

References:
RN [1]
RP NUCLEOTIDE SEQUENCE (ISOFORM 1), FUNCTION, AND TISSUE SPECIFICITY.
RC STRAIN=cv. Columbia;
RX MEDLINE=20223143; PubMed=10758501;
RA Gallego F., Fleck O., Li A., Wyrzykowska J., Tinland B.;
RT "AtRAD1, a plant homologue of human and yeast nucleotide excision
RT repair endonucleases, is involved in dark repair of UV damages and
RT recombination.";
RL Plant J. 21:507-518(2000).
RN [2]
RP NUCLEOTIDE SEQUENCE (ISOFORM 1), FUNCTION, AND TISSUE SPECIFICITY.
RC STRAIN=cv. Columbia;
RX MEDLINE=20223144; PubMed=10758502;
RA Liu Z., Hossain G.S., Islas-Osuna M.A., Mitchell D.L., Mount D.W.;
RT "Repair of UV damage in plants by nucleotide excision repair:
RT Arabidopsis UVH1 DNA repair gene is a homolog of Saccharomyces
RT cerevisiae Rad1.";
RL Plant J. 21:519-528(2000).
RN [3]
RP NUCLEOTIDE SEQUENCE (ISOFORM 1), FUNCTION, AND MUTAGENESIS OF GLY-756.
RC STRAIN=cv. Landsberg erecta;
RX MEDLINE=20484028; PubMed=11027708; DOI=10.1104/pp.124.2.579;
RA Fidantsef A.L., Mitchell D.L., Britt A.B.;
RT "The Arabidopsis UVH1 gene is a homolog of the yeast repair
RT endonuclease RAD1.";
RL Plant Physiol. 124:579-586(2000).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX MEDLINE=98290546; PubMed=9628582; DOI=10.1093/dnares/5.1.41;
RA Sato S., Kaneko T., Kotani H., Nakamura Y., Asamizu E., Miyajima N.,
RA Tabata S.;
RT "Structural analysis of Arabidopsis thaliana chromosome 5. IV.
RT Sequence features of the regions of 1,456,315 bp covered by nineteen
RT physically assigned P1 and TAC clones.";
RL DNA Res. 5:41-54(1998).
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
RC STRAIN=cv. Columbia;
RX MEDLINE=22954850; PubMed=14593172; DOI=10.1126/science.1088305;
RA Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J.,
RA Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F.,
RA Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G.,
RA Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J.,
RA Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y.,
RA Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P.,
RA Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F.,
RA Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M.,
RA Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J.,
RA Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T.,
RA Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y.,
RA Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.;
RT "Empirical analysis of transcriptional activity in the Arabidopsis
RT genome.";
RL Science 302:842-846(2003).
RN [6]
RP NUCLEOTIDE SEQUENCE OF 1-688, FUNCTION, AND ALTERNATIVE SPLICING.
RX PubMed=12383497; DOI=10.1016/S0378-1119(02)00869-7;
RA Vonarx E.J., Howlett N.G., Schiestl R.H., Kunz B.A.;
RT "Detection of Arabidopsis thaliana AtRAD1 cDNA variants and assessment
RT of function by expression in a yeast rad1 mutant.";
RL Gene 296:1-9(2002).
RN [7]
RP FUNCTION.
RX PubMed=12393748; DOI=10.1093/embo-reports/kvf211;
RA Dubest S., Gallego M.E., White C.I.;
RT "Role of the AtRad1p endonuclease in homologous recombination in
RT plants.";
RL EMBO Rep. 3:1049-1054(2002).
RN [8]
RP FUNCTION.
RX PubMed=11826311; DOI=10.1105/tpc.010258;
RA Li A., Schuermann D., Gallego F., Kovalchuk I., Tinland B.;
RT "Repair of damaged DNA by Arabidopsis cell extract.";
RL Plant Cell 14:263-273(2002).
RN [9]
RP FUNCTION.
RX PubMed=12554710; DOI=10.1093/jxb/erg069;
RA Hefner E., Preuss S.B., Britt A.B.;
RT "Arabidopsis mutants sensitive to gamma radiation include the
RT homologue of the human repair gene ERCC1.";
RL J. Exp. Bot. 54:669-680(2003).

Feature:
CHAIN 1 956 DNA repair endonuclease UVH1.
/FTId=PRO_0000198862.
MOTIF 256 272 Nuclear localization signal (Potential).
COMPBIAS 13 52 Leu-rich.
VARSPLIC 689 727 DGLCMGSNSSTEFPASSTQNSLTRKAGGRKELEKETQVI
-> FLFPAFFSSILCYKLGIRMGSAWGRILLQSFQLGVHKT
H (in isoform 2).
/FTId=VSP_011871.
VARSPLIC 728 956 Missing (in isoform 2).
/FTId=VSP_011872.
VARSPLIC 781 783 GRL -> VFE (in isoform 3).
/FTId=VSP_011873.
VARSPLIC 784 956 Missing (in isoform 3).
/FTId=VSP_011874.
MUTAGEN 756 756 G->D: Loss of DNA repair activity.
CONFLICT 22 22 L -> V (in Ref. 3).
CONFLICT 117 117 S -> A (in Ref. 3).
CONFLICT 147 147 S -> G (in Ref. 3).
CONFLICT 434 434 D -> G (in Ref. 6).
CONFLICT 544 544 P -> L (in Ref. 3).
CONFLICT 548 548 R -> Q (in Ref. 3).
CONFLICT 559 559 T -> A (in Ref. 3).
CONFLICT 720 720 L -> V (in Ref. 3).
CONFLICT 837 837 F -> S (in Ref. 3).

Comments:
-!- FUNCTION: Seems to be involved in nucleotide excision repair (NER)
of damaged DNA (dark repair mechanism). Involved in repair of UV
light, and probably oxidative damage. The UVH1/RAD1-ERCC1/RAD10
complex may act as an endonuclease making DNA incision 5' to the
lesion site. In vitro, is implicated in double strand breaks
(DSBs) repair and is required for homologous recombination in the
presence of non-homologous overhangs. May mediate the induction of
a DNA-damage sensitive cell-cycle checkpoint during the G2 phase.
-!- SUBUNIT: Heterodimer with ERCC1/RAD10 (Probable).
-!- SUBCELLULAR LOCATION: Nucleus (Probable).
-!- ALTERNATIVE PRODUCTS:
Event=Alternative splicing; Named isoforms=3;
Name=1; Synonyms=AtRAD1-1;
IsoId=Q9LKI5-1; Sequence=Displayed;
Name=2; Synonyms=AtRAD1-2, AtRAD1-4;
IsoId=Q9LKI5-2; Sequence=VSP_011871, VSP_011872;
Note=Inactive;
Name=3; Synonyms=AtRAD1-3;
IsoId=Q9LKI5-3; Sequence=VSP_011873, VSP_011874;
Note=Inactive;
-!- TISSUE SPECIFICITY: Isoform 1 and isoform 2 are widely expressed,
predominantly in flowers, meristems and stems. Isoform 3 is
detected at low levels.
-!- SIMILARITY: Belongs to the XPF family.
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Sequence length: 956

     MALKYHQQII SDLLEDSNGG LLILSSGLSL AKLIASLLIL HSPSQGTLLL LLSPAAQSLK
     SRIIHYISSL DSPTPTEITA DLPANQRYSL YTSGSPFFIT PRILIVDLLT QRIPVSSLAG
     IFILNAHSIS ETSTEAFIIR IVKSLNSSAY IRAFSDRPQA MVSGFAKTER TMRALFLRKI
     HLWPRFQLDV SQELEREPPE VVDIRVSMSN YMVGIQKAII EVMDACLKEM KKTNKVDVDD
     LTVESGLFKS FDEIVRRQLD PIWHTLGKRT KQLVSDLKTL RKLLDYLVRY DAVSFLKFLD
     TLRVSESYRS VWLFAESSYK IFDFAKKRVY RLVKASDVKS KEHVKNKSGK KRNSKGETDS
     VEAVGGETAT NVATGVVVEE VLEEAPKWKV LREILEETQE ERLKQAFSEE DNSDNNGIVL
     VACKDERSCM QLEDCITNNP QKVMREEWEM YLLSKIELRS MQTPQKKKQK TPKGFGILDG
     VVPVTTIQNS EGSSVGRQEH EALMAAASSI RKLGKTTDMA SGNNNPEPHV DKASCTKGKA
     KKDPTSLRRS LRSCNKKTTN SKPEILPGPE NEEKANEAST SAPQEANAVR PSGAKKLPPV
     HFYALESDQP ILDILKPSVI IVYHPDMGFV RELEVYKAEN PLRKLKVYFI FYDESTEVQK
     FEASIRRENE AFESLIRQKS SMIIPVDQDG LCMGSNSSTE FPASSTQNSL TRKAGGRKEL
     EKETQVIVDM REFMSSLPNV LHQKGMKIIP VTLEVGDYIL SPSICVERKS IQDLFQSFTS
     GRLFHQVEMM SRYYRIPVLL IEFSQDKSFS FQSSSDISDD VTPYNIISKL SLLVLHFPRL
     RLLWSRSLHA TAEIFTTLKS NQDEPDETRA IRVGVPSEEG IIENDIRAEN YNTSAVEFLR
     RLPGVSDANY RSIMEKCKSL AELASLPVET LAELMGGHKV AKSLREFLDA KYPTLL

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