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Description:
DNA repair helicase XPB1 (EC 3.6.1.-) (XPB homolog 1) (ERCC3 homolog1) (RAD25 homolog 1) (AtXPB1) (Protein araXPB).
Molecular weight: 87062
View which proteins in this organism that is involved with DNA Repair;
classified after biological processes (using data from the GOA project):
DNA repair( GO:0006281 ) nucleotide-excision repair (and GO:0045001, a synonym)( GO:0006289 )
Important dates:
04-JAN-2005, integrated into UniProtKB/Swiss-Prot.
01-MAR-2001, sequence version 3.
07-MAR-2006, entry version 38.
Phylogenetic order:
Eukaryota Viridiplantae Streptophyta Embryophyta Tracheophyta Spermatophyta Magnoliophyta eudicotyledons core eudicotyledons rosids eurosids II Brassicales Brassicaceae Arabidopsis.
To calculate the pI (Isoelectric point - the pH where a protein has a neutral charge),
go to this page and enter the protein ID (e.g 3MG_ECOLI): http://us.expasy.org/tools/pi_tool.html
Links to references in other databases for protein XPB1_ARATH:
| Database | Pointer | Add. info#1 | Add. info#2 |
| EMBL | U29168 | AAC49987.2.1 | - |
| EMBL | AY550923 | AAT36215.1 | - |
| EMBL | AB006707 | BAB08509.1 | ALT_SEQ |
| EMBL | AY039547 | AAK62602.1 | - |
| EMBL | AY113008 | AAM47316.1 | - |
| PIR | S71206 | S71206. | |
| GenomeReviews | BA000015_GR | AT5G41370.1 | |
| GeneFarm | 4453 | 455.1 | |
| TAIR | At5g41370 | -.1 | |
| InterPro | IPR011545 | DEAD/DEAH_N. | |
| InterPro | IPR001650 | Helicase_C. | |
| InterPro | IPR001161 | XPB_DNA_repair. | |
| PANTHER | PTHR11274 | XPB_DNA_repair.1 | 1. |
| Pfam | PF00270 | DEAD | 1. |
| Pfam | PF00271 | Helicase_C | 1. |
| PRINTS | PR00851 | XRODRMPGMNTB. | |
| SMART | SM00487 | DEXDc | 1. |
| SMART | SM00490 | HELICc | 1. |
| TIGRFAMs | TIGR00603 | rad25 | 1. |
Keywords:
ATP-binding; DNA damage; DNA repair; DNA-binding; Helicase; Hydrolase; Nuclear protein; Nucleotide-binding; Transcription; Transcription regulation.
References:
RN [1]
RP NUCLEOTIDE SEQUENCE, AND TISSUE SPECIFICITY.
RC STRAIN=cv. Columbia;
RX MEDLINE=98201615; PubMed=9524267; DOI=10.1016/S0378-1119(97)00656-2;
RA Ribeiro D.T., Machado C.R., Costa R.M.A., Praekelt U.M.,
RA Van Sluys M.-A., Menck C.F.M.;
RT "Cloning of a cDNA from Arabidopsis thaliana homologous to the human
RT XPB gene.";
RL Gene 208:207-213(1998).
RN [2]
RP NUCLEOTIDE SEQUENCE.
RC STRAIN=cv. Landsberg erecta;
RA Morgante P.G., Berra C.M., Nakabashi M., Costa R.M.A., Menck C.F.M.,
RA Van Sluys M.-A.;
RT "Functional XPB/RAD25 redundancy in Arabidopsis genome:
RT characterization of AtXPB2 and expression analysis.";
RL Submitted (FEB-2004) to the EMBL/GenBank/DDBJ databases.
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX MEDLINE=98069011; PubMed=9405937; DOI=10.1093/dnares/4.4.291;
RA Kotani H., Nakamura Y., Sato S., Kaneko T., Asamizu E., Miyajima N.,
RA Tabata S.;
RT "Structural analysis of Arabidopsis thaliana chromosome 5. II.
RT Sequence features of the regions of 1,044,062 bp covered by thirteen
RT physically assigned P1 clones.";
RL DNA Res. 4:291-300(1997).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RX MEDLINE=22954850; PubMed=14593172; DOI=10.1126/science.1088305;
RA Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J.,
RA Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F.,
RA Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G.,
RA Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J.,
RA Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y.,
RA Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P.,
RA Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F.,
RA Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M.,
RA Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J.,
RA Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T.,
RA Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y.,
RA Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.;
RT "Empirical analysis of transcriptional activity in the Arabidopsis
RT genome.";
RL Science 302:842-846(2003).
RN [5]
RP FUNCTION.
RX MEDLINE=21605276; PubMed=11737776;
RA Costa R.M.A., Morgante P.G., Berra C.M., Nakabashi M., Bruneau D.,
RA Bouchez D., Sweder K.S., Van Sluys M.-A., Menck C.F.M.;
RT "The participation of AtXPB1, the XPB/RAD25 homologue gene from
RT Arabidopsis thaliana, in DNA repair and plant development.";
RL Plant J. 28:385-395(2001).
Feature:
CHAIN 1 767 DNA repair helicase XPB1.
/FTId=PRO_0000101991.
NP_BIND 306 313 ATP (By similarity).
MOTIF 408 411 DEVH box.
MOTIF 750 766 Nuclear localization signal (Potential).
Comments:
-!- FUNCTION: Putative ATP-dependent 3'-5' DNA helicase involved in
nucleotide excision repair (NER) of DNA. May be involved in RNA
transcription by RNA polymerase II. Acts by opening DNA around
either the RNA transcription start site or the DNA damage (By
similarity). Required during the early stages of development,
including seed germination.
-!- SUBCELLULAR LOCATION: Nucleus (Probable).
-!- TISSUE SPECIFICITY: Expressed ubiquitously.
-!- SIMILARITY: Belongs to the helicase family. RAD25/XPB subfamily.
-!- CAUTION: Ref.3 sequence differs from that shown due to erroneous
gene model prediction.
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Sequence length: 767
MGNGERGRPN KKMKYGGKDD QKMKNIQNAE DYYDDADEDS RDGEGEEKKR DFTKLELKPD
HGNRPLWACA DGRIFLETFS PLYKQAYDFL IAIAEPVCRP ESMHEYNLTP HSLYAAVSVG
LETETIISVL NKLSKTKLPK EMIEFIHAST ANYGKVKLVL KKNRYFIESP FPEVLKRLLS
DDVINRARFS SEPYYGGDGF SVGRTCGELE AGPGELLNEA EFAAAAEEKE THSFEIDPAQ
VENVKQRCLP NALNYPMLEE YDFRNDNVNP DLDMELKPHA QPRPYQEKSL SKMFGNGRAR
SGIIVLPCGA GKSLVGVSAA ARIKKSCLCL ATNAVSVDQW AFQFKLWSTI RDDQICRFTS
DSKERFRGNA GVVVTTYNMV AFGGKRSEES EKIIEEMRNR EWGLLLMDEV HVVPAHMFRK
VISITKSHCK LGLTATLVRE DERITDLNFL IGPKLYEANW LDLVKGGFIA NVQCAEVWCP
MTKEFFAEYL KKENSKKKQA LYVMNPNKFR ACEFLIRFHE QQRGDKIIVF ADNLFALTEY
AMKLRKPMIY GATSHIERTK ILEAFKTSKD VNTVFLSKVG DNSIDIPEAN VIIQISSHAG
SRRQEAQRLG RILRAKGKLE DRMAGGKEEY NAFFYSLVST DTQEMYYSTK RQQFLIDQGY
SFKVITSLPP PDAGSSLSYH SQEEQLSLLG KVMNAGDDLV GLEQLEEDTD GMALQKARRS
MGSMSVMSGS KGMVYMEYNS GRHKSGQQFK KPKDPTKRHN LFKKRYV