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Description:
LexA repressor (EC 3.4.21.88).
Molecular weight: 25438
View which proteins in this organism that is involved with DNA Repair;
classified after biological processes (using data from the GOA project):
DNA repair( GO:0006281 )
Important dates:
01-MAR-2004, integrated into UniProtKB/Swiss-Prot.
01-JUN-2003, sequence version 1.
07-MAR-2006, entry version 19.
Phylogenetic order:
Bacteria Proteobacteria Alphaproteobacteria Rhizobiales Bradyrhizobiaceae Bradyrhizobium.
To calculate the pI (Isoelectric point - the pH where a protein has a neutral charge),
go to this page and enter the protein ID (e.g 3MG_ECOLI): http://us.expasy.org/tools/pi_tool.html
Links to references in other databases for protein LEXA_BRAJA:
| Database | Pointer | Add. info#1 | Add. info#2 |
| EMBL | BA000040 | BAC50091.1 | - |
| GenomeReviews | BA000040_GR | blr4826.1 | |
| HAMAP | MF_00015 | - | 1. |
| InterPro | IPR006199 | LexA_DNA_bd. | |
| InterPro | IPR006200 | Pept_S24_LexA. | |
| InterPro | IPR006198 | Pept_S24_S26. | |
| InterPro | IPR006197 | Pept_S24_SOS. | |
| InterPro | IPR011991 | Wing_hlx_DNA_bd. | |
| Pfam | PF01726 | LexA_DNA_bind | 1. |
| Pfam | PF00717 | Peptidase_S24 | 1. |
| PRINTS | PR00726 | LEXASERPTASE. | |
| TIGRFAMs | TIGR00498 | lexA | 1. |
Keywords:
Autocatalytic cleavage; Complete proteome; DNA damage; DNA repair; DNA replication; DNA-binding; Hydrolase; Repressor; SOS response; Transcription; Transcription regulation.
References:
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=USDA 110;
RX MEDLINE=22484998; PubMed=12597275; DOI=10.1093/dnares/9.6.189;
RA Kaneko T., Nakamura Y., Sato S., Minamisawa K., Uchiumi T.,
RA Sasamoto S., Watanabe A., Idesawa K., Iriguchi M., Kawashima K.,
RA Kohara M., Matsumoto M., Shimpo S., Tsuruoka H., Wada T., Yamada M.,
RA Tabata S.;
RT "Complete genomic sequence of nitrogen-fixing symbiotic bacterium
RT Bradyrhizobium japonicum USDA110.";
RL DNA Res. 9:189-197(2002).
Feature:
CHAIN 1 231 LexA repressor.
/FTId=PRO_0000170017.
DNA_BIND 26 46 H-T-H motif (By similarity).
ACT_SITE 152 152 Involved in auto-cleavage (By
similarity).
ACT_SITE 190 190 Involved in auto-cleavage (By
similarity).
SITE 117 118 Cleavage (auto-) (By similarity).
Comments:
-!- FUNCTION: Represses a number of genes involved in the response to
DNA damage (SOS response), including recA and lexA. In the
presence of single-stranded DNA, recA interacts with lexA causing
an autocatalytic cleavage which disrupts the DNA-binding part of
lexA, leading to derepression of the SOS regulon and eventually
DNA repair (By similarity).
-!- CATALYTIC ACTIVITY: Hydrolysis of Ala-|-Gly bond in repressor
lexA.
-!- SUBUNIT: Homodimer (By similarity).
-!- SIMILARITY: Belongs to the peptidase S24 family.
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Sequence length: 231
MLTRKQYELL RFISERLKES GVPPSFDEMK DALDLRSKSG IHRLITALEE RGFIRRLPNR
ARAIEVIKLP ELQAAAGNRR GFTPSVIEGN LGKVRASSSA DEGERPVAVP VMGRIAAGTP
IEALQTRSHT ISVPPDMLGS GEHYALEVRG DSMVEAGILD GDMALIQRNE SADTGDIVVA
LIDDEEATLK RFRRRGASIA LEPANAAYEV RILPPNRVKI QGKLIGLYRK Y