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Description:
LexA repressor (EC 3.4.21.88).
Molecular weight: 25667
View which proteins in this organism that is involved with DNA Repair;
classified after biological processes (using data from the GOA project):
DNA repair( GO:0006281 )
Important dates:
25-NOV-2002, integrated into UniProtKB/Swiss-Prot.
01-DEC-2001, sequence version 1.
07-MAR-2006, entry version 30.
Phylogenetic order:
Bacteria Proteobacteria Alphaproteobacteria Rhizobiales Rhizobiaceae Sinorhizobium.
To calculate the pI (Isoelectric point - the pH where a protein has a neutral charge),
go to this page and enter the protein ID (e.g 3MG_ECOLI): http://us.expasy.org/tools/pi_tool.html
Links to references in other databases for protein LEXA_RHIME:
| Database | Pointer | Add. info#1 | Add. info#2 |
| EMBL | AF251128 | AAQ14269.1 | - |
| EMBL | AL591787 | CAC46189.1 | - |
| HSSP | P03033 | 1JHC | |
| GenomeReviews | AL591688_GR | R01610.1 | |
| BioCyc | SMEL382:SMC01183-MONOMER | -.1 | |
| HAMAP | MF_00015 | - | 1. |
| InterPro | IPR006199 | LexA_DNA_bd. | |
| InterPro | IPR006200 | Pept_S24_LexA. | |
| InterPro | IPR006198 | Pept_S24_S26. | |
| InterPro | IPR006197 | Pept_S24_SOS. | |
| InterPro | IPR011991 | Wing_hlx_DNA_bd. | |
| Pfam | PF01726 | LexA_DNA_bind | 1. |
| Pfam | PF00717 | Peptidase_S24 | 1. |
| PRINTS | PR00726 | LEXASERPTASE. | |
| TIGRFAMs | TIGR00498 | lexA | 1. |
Keywords:
Autocatalytic cleavage; Complete proteome; DNA damage; DNA repair; DNA replication; DNA-binding; Hydrolase; Repressor; SOS response; Transcription; Transcription regulation.
References:
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RA Campoy S., Tapias A., Mazon G., Barbe J.;
RT "The lexA gene of Rhizobium meliloti.";
RL Submitted (MAR-2000) to the EMBL/GenBank/DDBJ databases.
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=1021;
RX MEDLINE=21396507; PubMed=11481430; DOI=10.1073/pnas.161294398;
RA Capela D., Barloy-Hubler F., Gouzy J., Bothe G., Ampe F., Batut J.,
RA Boistard P., Becker A., Boutry M., Cadieu E., Dreano S., Gloux S.,
RA Godrie T., Goffeau A., Kahn D., Kiss E., Lelaure V., Masuy D.,
RA Pohl T., Portetelle D., Puehler A., Purnelle B., Ramsperger U.,
RA Renard C., Thebault P., Vandenbol M., Weidner S., Galibert F.;
RT "Analysis of the chromosome sequence of the legume symbiont
RT Sinorhizobium meliloti strain 1021.";
RL Proc. Natl. Acad. Sci. U.S.A. 98:9877-9882(2001).
Feature:
CHAIN 1 238 LexA repressor.
/FTId=PRO_0000170076.
DNA_BIND 26 46 H-T-H motif (By similarity).
ACT_SITE 158 158 Involved in auto-cleavage (By
similarity).
ACT_SITE 196 196 Involved in auto-cleavage (By
similarity).
SITE 123 124 Cleavage (auto-) (By similarity).
CONFLICT 201 201 K -> R (in Ref. 1).
CONFLICT 227 227 Q -> K (in Ref. 1).
Comments:
-!- FUNCTION: Represses a number of genes involved in the response to
DNA damage (SOS response), including recA and lexA. In the
presence of single-stranded DNA, recA interacts with lexA causing
an autocatalytic cleavage which disrupts the DNA-binding part of
lexA, leading to derepression of the SOS regulon and eventually
DNA repair (By similarity).
-!- CATALYTIC ACTIVITY: Hydrolysis of Ala-|-Gly bond in repressor
lexA.
-!- SUBUNIT: Homodimer (By similarity).
-!- SIMILARITY: Belongs to the peptidase S24 family.
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Sequence length: 238
MLTRKQQELL LFIHERMKES GVPPSFDEMK DALDLASKSG IHRLITALEE RGFIRRLPNR
ARALEVIKLP EAYAGASQVR RGFSPSVIEG SLGKPAAPPP APKPAPPAEA ASVAVPVMGR
IAAGVPISAI QNNMHDISVP VEMIGSGEHY ALEIKGDSMI EAGILDGDTV IIRNGSTASP
GDIVVALIDD EEATLKRFRR KGASIALEAA NPAYETRIFG PDRVKIQGRL VGLIRRYH