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Description:
Crossover junction endodeoxyribonuclease ruvC (EC 3.1.22.4) (Hollidayjunction nuclease ruvC) (Holliday juction resolvase ruvC).
Molecular weight: 18022
View which proteins in this organism that is involved with DNA Repair;
classified after biological processes (using data from the GOA project):
DNA repair( GO:0006281 )
Important dates:
15-MAY-2002, integrated into UniProtKB/Swiss-Prot.
01-DEC-2001, sequence version 1.
07-MAR-2006, entry version 22.
Phylogenetic order:
Bacteria Proteobacteria Alphaproteobacteria Rhizobiales Rhizobiaceae Sinorhizobium.
To calculate the pI (Isoelectric point - the pH where a protein has a neutral charge),
go to this page and enter the protein ID (e.g 3MG_ECOLI): http://us.expasy.org/tools/pi_tool.html
Links to references in other databases for protein RUVC_RHIME:
| Database | Pointer | Add. info#1 | Add. info#2 |
| EMBL | AL591791 | CAC47330.1 | - |
| HSSP | P24239 | 1HJR | |
| GenomeReviews | AL591688_GR | R02751.1 | |
| BioCyc | SMEL382:SMC03967-MONOMER | -.1 | |
| HAMAP | MF_00034 | - | 1. |
| InterPro | IPR012337 | RNaseH_fold. | |
| InterPro | IPR002176 | RuvC. | |
| Pfam | PF02075 | RuvC | 1. |
| PRINTS | PR00696 | RSOLVASERUVC. | |
| ProDom | PD008333 | RuvC | 1. |
| TIGRFAMs | TIGR00228 | ruvC | 1. |
| PROSITE | PS01321 | RUVC | FALSE_NEG. |
Keywords:
Complete proteome; DNA damage; DNA recombination; DNA repair; Hydrolase; Magnesium; Metal-binding; Nuclease.
References:
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=1021;
RX MEDLINE=21396507; PubMed=11481430; DOI=10.1073/pnas.161294398;
RA Capela D., Barloy-Hubler F., Gouzy J., Bothe G., Ampe F., Batut J.,
RA Boistard P., Becker A., Boutry M., Cadieu E., Dreano S., Gloux S.,
RA Godrie T., Goffeau A., Kahn D., Kiss E., Lelaure V., Masuy D.,
RA Pohl T., Portetelle D., Puehler A., Purnelle B., Ramsperger U.,
RA Renard C., Thebault P., Vandenbol M., Weidner S., Galibert F.;
RT "Analysis of the chromosome sequence of the legume symbiont
RT Sinorhizobium meliloti strain 1021.";
RL Proc. Natl. Acad. Sci. U.S.A. 98:9877-9882(2001).
Feature:
CHAIN 1 170 Crossover junction endodeoxyribonuclease
ruvC.
/FTId=PRO_0000183126.
METAL 11 11 Magnesium (By similarity).
METAL 71 71 Magnesium (By similarity).
METAL 143 143 Magnesium (By similarity).
METAL 146 146 Magnesium (By similarity).
Comments:
-!- FUNCTION: Nuclease that resolves Holliday junction intermediates
in genetic recombination. Cleaves the cruciform structure in
supercoiled DNA by nicking to strands with the same polarity at
sites symmetrically opposed at the junction in the homologous arms
and leaves a 5'terminal phosphate and a 3'terminal hydroxyl group
(By similarity).
-!- CATALYTIC ACTIVITY: Endonucleolytic cleavage at a junction such as
a reciprocal single-stranded crossover between two homologous DNA
duplexes (Holliday junction).
-!- COFACTOR: Binds 1 magnesium ion per subunit (By similarity).
-!- SIMILARITY: Belongs to the ruvC family.
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Sequence length: 170
MQNTIRIIGI DPGLRRTGWG VIETLGNSLR FVASGTVTSD GELDLASRLC QLHDGLAEVV
HGYQPHEAAV EQTFVNKDAT ATLKLGQARG IAMLVPARAG LRVAEYAPNA VKKAVIGVGH
GEKQQIHMML KVLMPKAEFK GNDAADALAI AICHAHNRQA VTSRLAALAG