Description:
DNA-(apurinic or apyrimidinic site) lyase (EC 4.2.99.18) (Class IIapurinic/apyrimidinic(AP)-endonuclease).
Molecular weight: 41251
View which proteins in this organism that is involved with DNA Repair;
classified after biological processes (using data from the GOA project):
DNA repair( GO:0006281 )
Important dates:
01-OCT-1996, integrated into UniProtKB/Swiss-Prot.
01-OCT-1996, sequence version 1.
07-FEB-2006, entry version 39.
Phylogenetic order:
Eukaryota Mycetozoa Dictyosteliida Dictyostelium.
To calculate the pI (Isoelectric point - the pH where a protein has a neutral charge),
go to this page and enter the protein ID (e.g 3MG_ECOLI): http://us.expasy.org/tools/pi_tool.html
Links to references in other databases for protein APEA_DICDI:
| Database | Pointer | Add. info#1 | Add. info#2 |
| EMBL | U31631 | AAC47024.1 | - |
| PIR | S68268 | S68268. | |
| HSSP | P27695 | 1E9N | |
| DictyBase | DDB0185018 | apeA.1 | |
| InterPro | IPR000097 | APendonclse1. | |
| InterPro | IPR005135 | Exo_endo_phos. | |
| InterPro | IPR004808 | ExoIII_xth. | |
| Pfam | PF03372 | Exo_endo_phos | 1. |
| TIGRFAMs | TIGR00195 | exoDNase_III | 1. |
| TIGRFAMs | TIGR00633 | xth | 1. |
| PROSITE | PS00726 | AP_NUCLEASE_F1_1 | 1. |
| PROSITE | PS00727 | AP_NUCLEASE_F1_2 | 1. |
| PROSITE | PS00728 | AP_NUCLEASE_F1_3 | 1. |
Keywords:
DNA damage; DNA repair; Lyase; Magnesium; Metal-binding; Nuclear protein.
References:
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX MEDLINE=96226184; PubMed=8657579; DOI=10.1093/nar/24.10.1950;
RA Freeland T.M., Guyer R.B., Ling A.Z., Deering R.A.;
RT "Apurinic/apyrimidinic (AP) endonuclease from Dictyostelium
RT discoideum: cloning, nucleotide sequence and induction by sublethal
RT levels of DNA damaging agents.";
RL Nucleic Acids Res. 24:1950-1953(1996).
Feature:
CHAIN 1 361 DNA-(apurinic or apyrimidinic site)
lyase.
/FTId=PRO_0000200016.
MOTIF 41 44 Nuclear localization signal (Potential).
COMPBIAS 27 39 Poly-Glu.
COMPBIAS 70 80 Poly-Glu.
ACT_SITE 351 351 Proton acceptor (By similarity).
METAL 139 139 Magnesium or manganese (By similarity).
Comments:
-!- CATALYTIC ACTIVITY: The C-O-P bond 3' to the apurinic or
apyrimidinic site in DNA is broken by a beta-elimination reaction,
leaving a 3'-terminal unsaturated sugar and a product with a
terminal 5'-phosphate.
-!- SUBCELLULAR LOCATION: Nucleus (Potential).
-!- INDUCTION: By DNA-damaging agents including UV, MNNG, gamma rays,
bleomycin and streptozotocin.
-!- SIMILARITY: Belongs to the DNA repair enzymes AP/exoA family.
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Sequence length: 361
MTSRTKKLKM DEEEILKKED GSETTSEEEK EEVEEEEEED KKRKLVKKTP AKKAPAKKAA
AKKKSKDEDE DEEEKEEEEE TNKTTASVSI AIDNLDEPKV EENQMKIISW NVAGFKSVLS
KGFTECVEKE NPDVLCLQET KINPSNIKKD QMPKGYEYHF IEADQKGHHG TGVLTKKKPN
AITFGIGIAK HDNEGRVITL EYVQFYIVNT YIPNAGTRGL QRLDYRIKEW DVDFQAYLEK
LNATKPIIWC GDLNVAHTEI DLKNPKTNKK SAGFTIEERT SFSNFLEKGY VDSYRHFNPG
KEGSYTFWSY LGGGRSKNVG WRLDYFVVSK RLMDSIKISP FHRTSVMGSD HCPIGVVVDL
N