|
|
|
|
|
|
Description:
DNA ligase (EC 6.5.1.1) (Polydeoxyribonucleotide synthase [ATP]).
Molecular weight: 86581
View which proteins in this organism that is involved with DNA Repair;
classified after biological processes (using data from the GOA project):
DNA repair( GO:0006281 )
Important dates:
01-OCT-1989, integrated into UniProtKB/Swiss-Prot.
01-OCT-1989, sequence version 1.
07-FEB-2006, entry version 51.
Phylogenetic order:
Eukaryota Fungi Ascomycota Schizosaccharomycetes Schizosaccharomycetales Schizosaccharomycetaceae Schizosaccharomyces.
To calculate the pI (Isoelectric point - the pH where a protein has a neutral charge),
go to this page and enter the protein ID (e.g 3MG_ECOLI): http://us.expasy.org/tools/pi_tool.html
Links to references in other databases for protein DNLI_SCHPO:
| Database | Pointer | Add. info#1 | Add. info#2 |
| EMBL | X05107 | CAA28754.1 | - |
| EMBL | Z94864 | CAB08176.1 | - |
| EMBL | Z95334 | CAB08595.1 | - |
| PIR | A29066 | A29066. | |
| GeneDB_Spombe | SPAC20G8.01 | -.1 | |
| BioCyc | SPOM-XXX-01:SPOM-XXX-01-001954-MONOMER | -.1 | |
| GO | GO:0005739 | C:mitochondrion | IDA. |
| GO | GO:0005634 | C:nucleus | IDA. |
| GO | GO:0003910 | F:DNA ligase (ATP) activity | IGI. |
| GO | GO:0005515 | F:protein binding | IPI. |
| GO | GO:0043137 | P:DNA replication, removal of RNA primer | TAS. |
| GO | GO:0000723 | P:telomere maintenance | IMP. |
| InterPro | IPR000977 | DNA_ligase. | |
| InterPro | IPR012309 | DNA_ligase_A_C. | |
| InterPro | IPR012310 | DNA_ligase_A_M. | |
| InterPro | IPR012308 | DNA_ligase_A_N. | |
| PANTHER | PTHR10459 | DNA_ligase.1 | 1. |
| Pfam | PF04679 | DNA_ligase_A_C | 1. |
| Pfam | PF01068 | DNA_ligase_A_M | 1. |
| Pfam | PF04675 | DNA_ligase_A_N | 1. |
| TIGRFAMs | TIGR00574 | dnl1 | 1. |
| PROSITE | PS00697 | DNA_LIGASE_A1 | 1. |
| PROSITE | PS00333 | DNA_LIGASE_A2 | 1. |
| PROSITE | PS50160 | DNA_LIGASE_A3 | 1. |
Keywords:
ATP-binding; Cell cycle; Cell division; Complete proteome; DNA damage; DNA recombination; DNA repair; DNA replication; Ligase; Nuclear protein; Nucleotide-binding.
References:
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX MEDLINE=87161837; PubMed=3549293;
RA Barker D.G., White J.H.M., Johnston L.H.;
RT "Molecular characterisation of the DNA ligase gene, CDC17, from the
RT fission yeast Schizosaccharomyces pombe.";
RL Eur. J. Biochem. 162:659-667(1987).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=972;
RX MEDLINE=21848401; PubMed=11859360; DOI=10.1038/nature724;
RA Wood V., Gwilliam R., Rajandream M.A., Lyne M.H., Lyne R., Stewart A.,
RA Sgouros J.G., Peat N., Hayles J., Baker S.G., Basham D., Bowman S.,
RA Brooks K., Brown D., Brown S., Chillingworth T., Churcher C.M.,
RA Collins M., Connor R., Cronin A., Davis P., Feltwell T., Fraser A.,
RA Gentles S., Goble A., Hamlin N., Harris D.E., Hidalgo J., Hodgson G.,
RA Holroyd S., Hornsby T., Howarth S., Huckle E.J., Hunt S., Jagels K.,
RA James K.D., Jones L., Jones M., Leather S., McDonald S., McLean J.,
RA Mooney P., Moule S., Mungall K.L., Murphy L.D., Niblett D., Odell C.,
RA Oliver K., O'Neil S., Pearson D., Quail M.A., Rabbinowitsch E.,
RA Rutherford K.M., Rutter S., Saunders D., Seeger K., Sharp S.,
RA Skelton J., Simmonds M.N., Squares R., Squares S., Stevens K.,
RA Taylor K., Taylor R.G., Tivey A., Walsh S.V., Warren T., Whitehead S.,
RA Woodward J.R., Volckaert G., Aert R., Robben J., Grymonprez B.,
RA Weltjens I., Vanstreels E., Rieger M., Schaefer M., Mueller-Auer S.,
RA Gabel C., Fuchs M., Duesterhoeft A., Fritzc C., Holzer E., Moestl D.,
RA Hilbert H., Borzym K., Langer I., Beck A., Lehrach H., Reinhardt R.,
RA Pohl T.M., Eger P., Zimmermann W., Wedler H., Wambutt R., Purnelle B.,
RA Goffeau A., Cadieu E., Dreano S., Gloux S., Lelaure V., Mottier S.,
RA Galibert F., Aves S.J., Xiang Z., Hunt C., Moore K., Hurst S.M.,
RA Lucas M., Rochet M., Gaillardin C., Tallada V.A., Garzon A., Thode G.,
RA Daga R.R., Cruzado L., Jimenez J., Sanchez M., del Rey F., Benito J.,
RA Dominguez A., Revuelta J.L., Moreno S., Armstrong J., Forsburg S.L.,
RA Cerutti L., Lowe T., McCombie W.R., Paulsen I., Potashkin J.,
RA Shpakovski G.V., Ussery D., Barrell B.G., Nurse P.;
RT "The genome sequence of Schizosaccharomyces pombe.";
RL Nature 415:871-880(2002).
Feature:
CHAIN 1 768 DNA ligase.
/FTId=PRO_0000059585.
ACT_SITE 416 416 N6-AMP-lysine intermediate (By
similarity).
Comments:
-!- FUNCTION: This protein seals, during DNA replication, DNA
recombination and DNA repair, nicks in double-stranded DNA.
-!- CATALYTIC ACTIVITY: ATP + (deoxyribonucleotide)(n) +
(deoxyribonucleotide)(m) = AMP + diphosphate +
(deoxyribonucleotide)(n+m).
-!- SUBCELLULAR LOCATION: Nucleus.
-!- SIMILARITY: Belongs to the ATP-dependent DNA ligase family.
-----------------------------------------------------------------------
Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms
Distributed under the Creative Commons Attribution-NoDerivs License
-----------------------------------------------------------------------
Sequence length: 768
MRTVFSQIPR FKQVNQYIRM STRQSDISNF FISSASHKSE HVEVSQSSSD SKNVDGRSTS
EKRKVESVKL VDESKHNNHD DTGTQNVERE NNIVSEAKKQ KTLGSSSSSS DAVSSNNDSG
ASTPIPLPIK EPPLESNARN DKLKGHATFA EMVKAFTKIE NTSKRLEIID IMGTYFFGIL
RDHPSDLLAC VYLSINKLGP DYSGLELGIG ESIIMKAIGE STGQTLQQIK LSFHKVGDLG
LVAQTSRQNQ PTMFKPAALT IPFLFDSLKK IAQMSGNQSQ NRKIGVIKRL LSSCEGAEPK
YLIRALEGKL RLQLAEKTIL VALANATAQY HADKNGEKLS QQDRIEGEQI LRDVYCQLPS
YDLIVPHLIE HGLGTLRETC KLTPGIPTKP MLAKPTKQIS EVLNTFDQAA FTCEYKYDGE
RAQVHFTEDG KFYVFSRNSE NMSVRYPDIS VSVSKWKKPD ARSFILDCEA VGWDRDENKI
LPFQKLATRK RKDVKIGDIK VRACLFAFDI LYLNGQPLLE TPLNERRKLL YSMFQPSTGD
FTFAKHSDQK SIESIEEFLE ESVKDSCEGL MVKMLEGPDS HYEPSKRSRH WLKVKKDYLS
GVGDSLDLIV IGAYYGKGKR TSVYGAFLLG CYDPDTETVQ SICKLGTGFS EEHLETFYNQ
LKDIVISKKK DFYAHSDVPA HQPDVWFEPK YLWEVLAADL SLSPVYKAAI GYVQEDKGIS
LRFPRFIRIR EDKSWEDATT SEQVSEFYRS QVAYSQKEKE GSPAAEDY