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Description:
Crossover junction endonuclease eme1 (EC 3.1.22.-) (Essential meioticendonuclease 1).
Molecular weight: 82932
View which proteins in this organism that is involved with DNA Repair;
classified after biological processes (using data from the GOA project):
DNA repair( GO:0006281 )
Important dates:
13-APR-2004, integrated into UniProtKB/Swiss-Prot.
01-JUN-2001, sequence version 1.
07-MAR-2006, entry version 20.
Phylogenetic order:
Eukaryota Fungi Ascomycota Schizosaccharomycetes Schizosaccharomycetales Schizosaccharomycetaceae Schizosaccharomyces.
To calculate the pI (Isoelectric point - the pH where a protein has a neutral charge),
go to this page and enter the protein ID (e.g 3MG_ECOLI): http://us.expasy.org/tools/pi_tool.html
Links to references in other databases for protein EME1_SCHPO:
| Database | Pointer | Add. info#1 | Add. info#2 |
| EMBL | AL590605 | CAC36923.1 | - |
| GeneDB_Spombe | SPAPB1E7.06c | -.1 | |
| BioCyc | SPOM-XXX-01:SPOM-XXX-01-001755-MONOMER | -.1 | |
| GO | GO:0048476 | C:Holliday junction resolvase complex | TAS. |
| GO | GO:0008821 | F:crossover junction endodeoxyribonuclease ac... | IDA. |
| GO | GO:0031297 | P:collapsed replication fork processing | IMP. |
| GO | GO:0006281 | P:DNA repair | TAS. |
| GO | GO:0007131 | P:meiotic recombination | IDA. |
Keywords:
Complete proteome; Direct protein sequencing; DNA damage; DNA recombination; DNA repair; Endonuclease; Hydrolase; Magnesium; Meiosis; Metal-binding; Nuclear protein; Nuclease.
References:
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=972;
RX MEDLINE=21848401; PubMed=11859360; DOI=10.1038/nature724;
RA Wood V., Gwilliam R., Rajandream M.A., Lyne M.H., Lyne R., Stewart A.,
RA Sgouros J.G., Peat N., Hayles J., Baker S.G., Basham D., Bowman S.,
RA Brooks K., Brown D., Brown S., Chillingworth T., Churcher C.M.,
RA Collins M., Connor R., Cronin A., Davis P., Feltwell T., Fraser A.,
RA Gentles S., Goble A., Hamlin N., Harris D.E., Hidalgo J., Hodgson G.,
RA Holroyd S., Hornsby T., Howarth S., Huckle E.J., Hunt S., Jagels K.,
RA James K.D., Jones L., Jones M., Leather S., McDonald S., McLean J.,
RA Mooney P., Moule S., Mungall K.L., Murphy L.D., Niblett D., Odell C.,
RA Oliver K., O'Neil S., Pearson D., Quail M.A., Rabbinowitsch E.,
RA Rutherford K.M., Rutter S., Saunders D., Seeger K., Sharp S.,
RA Skelton J., Simmonds M.N., Squares R., Squares S., Stevens K.,
RA Taylor K., Taylor R.G., Tivey A., Walsh S.V., Warren T., Whitehead S.,
RA Woodward J.R., Volckaert G., Aert R., Robben J., Grymonprez B.,
RA Weltjens I., Vanstreels E., Rieger M., Schaefer M., Mueller-Auer S.,
RA Gabel C., Fuchs M., Duesterhoeft A., Fritzc C., Holzer E., Moestl D.,
RA Hilbert H., Borzym K., Langer I., Beck A., Lehrach H., Reinhardt R.,
RA Pohl T.M., Eger P., Zimmermann W., Wedler H., Wambutt R., Purnelle B.,
RA Goffeau A., Cadieu E., Dreano S., Gloux S., Lelaure V., Mottier S.,
RA Galibert F., Aves S.J., Xiang Z., Hunt C., Moore K., Hurst S.M.,
RA Lucas M., Rochet M., Gaillardin C., Tallada V.A., Garzon A., Thode G.,
RA Daga R.R., Cruzado L., Jimenez J., Sanchez M., del Rey F., Benito J.,
RA Dominguez A., Revuelta J.L., Moreno S., Armstrong J., Forsburg S.L.,
RA Cerutti L., Lowe T., McCombie W.R., Paulsen I., Potashkin J.,
RA Shpakovski G.V., Ussery D., Barrell B.G., Nurse P.;
RT "The genome sequence of Schizosaccharomyces pombe.";
RL Nature 415:871-880(2002).
RN [2]
RP PROTEIN SEQUENCE OF 376-390; 434-456 AND 695-709, FUNCTION, COFACTOR,
RP INTERACTION WITH MUS81, AND SUBCELLULAR LOCATION.
RX PubMed=11719193; DOI=10.1016/S0092-8674(01)00536-0;
RA Boddy M.N., Gaillard P.-H.L., McDonald W.H., Shanahan P.,
RA Yates J.R. III, Russell P.;
RT "Mus81-Eme1 are essential components of a Holliday junction
RT resolvase.";
RL Cell 107:537-548(2001).
RN [3]
RP FUNCTION, AND INTERACTION WITH MUS81.
RX PubMed=12084712; DOI=10.1074/jbc.M202120200;
RA Doe C.L., Ahn J.S., Dixon J., Whitby M.C.;
RT "Mus81-Eme1 and Rqh1 involvement in processing stalled and collapsed
RT replication forks.";
RL J. Biol. Chem. 277:32753-32759(2002).
RN [4]
RP FUNCTION.
RA Smith G.R., Boddy M.N., Shanahan P., Russell P.;
RT "Fission yeast Mus81.Eme1 Holliday junction resolvase is required for
RT meiotic crossing over but not for gene conversion.";
RL Genetics 165:2289-2293(2003).
RN [5]
RP FUNCTION.
RX PubMed=12473680; DOI=10.1074/jbc.M210006200;
RA Whitby M.C., Osman F., Dixon J.;
RT "Cleavage of model replication forks by fission yeast Mus81-Eme1 and
RT budding yeast Mus81-Mms4.";
RL J. Biol. Chem. 278:6928-6935(2003).
RN [6]
RP FUNCTION.
RA Gaillard P.-H.L., Noguchi E., Shanahan P., Russell P.;
RT "The endogenous Mus81-Eme1 complex resolves Holliday junctions by a
RT nick and counternick mechanism.";
RL Mol. Cell 12:747-759(2003).
RN [7]
RP FUNCTION.
RX PubMed=14527420; DOI=10.1016/S1097-2765(03)00343-5;
RA Osman F., Dixon J., Doe C.L., Whitby M.C.;
RT "Generating crossovers by resolution of nicked Holliday junctions: a
RT role for Mus81-Eme1 in meiosis.";
RL Mol. Cell 12:761-774(2003).
RN [8]
RP FUNCTION.
RX PubMed=15486206; DOI=10.1093/nar/gkh853;
RA Doe C.L., Osman F., Dixon J., Whitby M.C.;
RT "DNA repair by a Rad22-Mus81-dependent pathway that is independent of
RT Rhp51.";
RL Nucleic Acids Res. 32:5570-5581(2004).
Feature:
CHAIN 1 738 Crossover junction endonuclease eme1.
/FTId=PRO_0000086966.
Comments:
-!- FUNCTION: Interacts with mus81 to form a DNA structure-specific
endonuclease with substrate preference for branched DNA structures
with a 5'-end at the branch nick. Typical substrates include 3'-
flap structures, D-loops, replication forks and nicked Holliday
junctions. May be required in mitosis for the processing of
stalled or collapsed replication fork intermediates. May be
required in meiosis for the repair of meiosis-specific double
strand breaks subsequent to single-end invasion (SEI).
-!- CATALYTIC ACTIVITY: Endonucleolytic cleavage at a junction such as
a reciprocal single-stranded crossover between two homologous DNA
duplexes (Holliday junction).
-!- COFACTOR: Magnesium.
-!- SUBUNIT: Interacts with mus81.
-!- SUBCELLULAR LOCATION: Nucleus.
-!- MISCELLANEOUS: S.pombe appears to be critically dependent on the
mus81-eme1 endonuclease for the resolution of meiotic crossovers.
This may be due to the absence of an alternate pathway for
crossover resolution such as the MSH4-MSH5 pathway which exists in
S.cerevisiae and other eukaryotes.
-!- SIMILARITY: Belongs to the EME1/MMS4 family.
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Sequence length: 738
MTLQSTDSAI VIDSEADVDI SSSQASPSKV CRDNIALSEH NVITVLDTPQ RSTQCDSLLK
SFSTPLVSGS EDVLPSPRDA LNITNKKSVT DNLLLSLTSS NQSTNTNLNP SSRVEIINLN
SSPPNSLSSQ PKHQEFHLFH TPTIPRTTQL SSKTSSPIVI PDDNEQVASP LSKKAASLTS
SPLKDFQSSP PLSTVLQKSH SLHDILLDTN DDDHFPFTQS PLTKTKSFND ALTSSSSILK
PCMPSIASPT SNRLSHAPST PNLFPNQDSS NTIDLINNRS KTSVENQERT FNLTSDVHLD
SPTSPSKHSS IEPNTSQEDS FQELPSLNKL SIQSRAFKKM RLPKISRTTD TPPASTSNSN
KKNLDKLKKM RKLCSRSLEP YELDSNTQRK RKRYEDSLKK SKTLDKVDSL NRKMAKELDR
KNSKELQKIN KVKRTKEECL SEIILLSPDD WASSWYSTVR SQLDSYNCQF VVNSNQPKDS
IMWKRKVNNV FNSSTNRFEL SIEHEQIEPF ALLRLKCRDF IKYIEEDQAD TFFHEMSEKF
KGCKLILLLE GIPNYFKSLK AELNRQYAAA VNSGTRPLLF GSLSKYQNFT KEKLESEIVR
FSFEHSILIN TSNDEKETAQ WIVSFTGDIA LSRYKHFSKF SARASTTEIG HVKSADRIEN
SLNFMLRQIL RVTPNIANAI CDQFDSIPSL IHHLKTHGEE SLTNVVIQSS ISERNLGPVL
SRRIYNTFLC KEASSDAP