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Description:
ATP-dependent DNA helicase hus2/rqh1 (EC 3.6.1.-).
Molecular weight: 1496
View which proteins in this organism that is involved with DNA Repair;
classified after biological processes (using data from the GOA project):
DNA repair( GO:0006281 )
Important dates:
01-NOV-1995, integrated into UniProtKB/Swiss-Prot.
01-NOV-1995, sequence version 1.
07-FEB-2006, entry version 47.
Phylogenetic order:
Eukaryota Fungi Ascomycota Schizosaccharomycetes Schizosaccharomycetales Schizosaccharomycetaceae Schizosaccharomyces.
To calculate the pI (Isoelectric point - the pH where a protein has a neutral charge),
go to this page and enter the protein ID (e.g 3MG_ECOLI): http://us.expasy.org/tools/pi_tool.html
Links to references in other databases for protein HUS2_SCHPO:
| Database | Pointer | Add. info#1 | Add. info#2 |
| EMBL | Y09426 | CAA70577.1 | - |
| EMBL | Z54354 | CAA91177.1 | - |
| PIR | S62467 | S62467. | |
| HSSP | P10081 | 1FUK | |
| GeneDB_Spombe | SPAC2G11.12 | -.1 | |
| BioCyc | SPOM-XXX-01:SPOM-XXX-01-000804-MONOMER | -.1 | |
| GO | GO:0031422 | C:RecQ helicase-Topo III complex | IDA. |
| GO | GO:0043140 | F:ATP-dependent 3' to 5' DNA helicase activity | IDA. |
| GO | GO:0031297 | P:collapsed replication fork processing | TAS. |
| GO | GO:0006281 | P:DNA repair | TAS. |
| GO | GO:0000076 | P:DNA replication checkpoint | TAS. |
| GO | GO:0009650 | P:UV protection | IMP. |
| InterPro | IPR001410 | DEAD. | |
| InterPro | IPR011545 | DEAD/DEAH_N. | |
| InterPro | IPR002464 | DEAH_box. | |
| InterPro | IPR001650 | Helicase_C. | |
| InterPro | IPR002121 | HRDC. | |
| InterPro | IPR004589 | RecQ. | |
| PANTHER | PTHR13710 | RecQ.1 | 1. |
| Pfam | PF00270 | DEAD | 1. |
| Pfam | PF00271 | Helicase_C | 1. |
| Pfam | PF00570 | HRDC | 1. |
| SMART | SM00487 | DEXDc | 1. |
| SMART | SM00490 | HELICc | 1. |
| SMART | SM00341 | HRDC | 1. |
| TIGRFAMs | TIGR00614 | recQ_fam | 1. |
| PROSITE | PS00690 | DEAH_ATP_HELICASE | 1. |
| PROSITE | PS50967 | HRDC | 1. |
Keywords:
ATP-binding; Complete proteome; DNA damage; DNA repair; DNA-binding; Helicase; Hydrolase; Nuclear protein; Nucleotide-binding.
References:
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX MEDLINE=97327548; PubMed=9184215; DOI=10.1093/emboj/16.10.2682;
RA Stewart E.C., Chapman C.R., Al-Khodairy F., Carr A.M., Enoch T.;
RT "rqh1+, a fission yeast gene related to the Bloom's and Werner's
RT syndrome genes, is required for reversible S phase arrest.";
RL EMBO J. 16:2682-2692(1997).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=972;
RX MEDLINE=21848401; PubMed=11859360; DOI=10.1038/nature724;
RA Wood V., Gwilliam R., Rajandream M.A., Lyne M.H., Lyne R., Stewart A.,
RA Sgouros J.G., Peat N., Hayles J., Baker S.G., Basham D., Bowman S.,
RA Brooks K., Brown D., Brown S., Chillingworth T., Churcher C.M.,
RA Collins M., Connor R., Cronin A., Davis P., Feltwell T., Fraser A.,
RA Gentles S., Goble A., Hamlin N., Harris D.E., Hidalgo J., Hodgson G.,
RA Holroyd S., Hornsby T., Howarth S., Huckle E.J., Hunt S., Jagels K.,
RA James K.D., Jones L., Jones M., Leather S., McDonald S., McLean J.,
RA Mooney P., Moule S., Mungall K.L., Murphy L.D., Niblett D., Odell C.,
RA Oliver K., O'Neil S., Pearson D., Quail M.A., Rabbinowitsch E.,
RA Rutherford K.M., Rutter S., Saunders D., Seeger K., Sharp S.,
RA Skelton J., Simmonds M.N., Squares R., Squares S., Stevens K.,
RA Taylor K., Taylor R.G., Tivey A., Walsh S.V., Warren T., Whitehead S.,
RA Woodward J.R., Volckaert G., Aert R., Robben J., Grymonprez B.,
RA Weltjens I., Vanstreels E., Rieger M., Schaefer M., Mueller-Auer S.,
RA Gabel C., Fuchs M., Duesterhoeft A., Fritzc C., Holzer E., Moestl D.,
RA Hilbert H., Borzym K., Langer I., Beck A., Lehrach H., Reinhardt R.,
RA Pohl T.M., Eger P., Zimmermann W., Wedler H., Wambutt R., Purnelle B.,
RA Goffeau A., Cadieu E., Dreano S., Gloux S., Lelaure V., Mottier S.,
RA Galibert F., Aves S.J., Xiang Z., Hunt C., Moore K., Hurst S.M.,
RA Lucas M., Rochet M., Gaillardin C., Tallada V.A., Garzon A., Thode G.,
RA Daga R.R., Cruzado L., Jimenez J., Sanchez M., del Rey F., Benito J.,
RA Dominguez A., Revuelta J.L., Moreno S., Armstrong J., Forsburg S.L.,
RA Cerutti L., Lowe T., McCombie W.R., Paulsen I., Potashkin J.,
RA Shpakovski G.V., Ussery D., Barrell B.G., Nurse P.;
RT "The genome sequence of Schizosaccharomyces pombe.";
RL Nature 415:871-880(2002).
RN [3]
RP FUNCTION, INTERACTION WITH TOP3, SUBCELLULAR LOCATION, AND MUTAGENESIS
RP OF THR-543.
RX MEDLINE=22610353; PubMed=12724426;
RX DOI=10.1128/MCB.23.10.3692-3705.2003;
RA Laursen L.V., Ampatzidou E., Andersen A.H., Murray J.M.;
RT "Role for the fission yeast RecQ helicase in DNA repair in G2.";
RL Mol. Cell. Biol. 23:3692-3705(2003).
Feature:
CHAIN 1 1328 ATP-dependent DNA helicase hus2/rqh1.
/FTId=PRO_0000205058.
DOMAIN 1115 1195 HRDC.
NP_BIND 555 562 ATP (By similarity).
MOTIF 651 654 DEAH box.
COMPBIAS 225 236 Poly-Asn.
COMPBIAS 321 332 Poly-Asn.
MUTAGEN 543 543 T->I: Loss of helicase activity.
Comments:
-!- FUNCTION: Has a role in the repair of UV-induced DNA damage in G2
via recombination-mediated repair. Also has a role in the repair
of infrared-induced double DNA strand breaks. Exhibits an ATP-
dependent DNA-helicase activity that unwinds single- and double-
stranded DNA in a 3'-5' direction.
-!- SUBUNIT: Interacts with top3.
-!- SUBCELLULAR LOCATION: Nucleus.
-!- SIMILARITY: Belongs to the helicase family. RecQ subfamily.
-!- SIMILARITY: Contains 1 HRDC domain.
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Sequence length: 1328
MTVTKTNLNR HLDWFFRESP QKIENVTSPI KTLDFVKVKV SSSDIVVKDS IPHKSKNVFD
DFDDGYAIDL TEEHQSSSLN NLKWKDVEGP NILKPIKKIA VPASESEEDF DDVDEEMLRA
AEMEVFQSCQ PLAVNTADTT VSHSTSSSNV PRSLNKIHDP SRFIKDNDVE NRIHVSSASK
VASISNTSKP NPIVSENPIS ATSVSIEIPI KPKELSNNLP FPRLNNNNTN NNNDNNAIEK
RDSASPTPSS VSSQISIDFS TWPHQNLLQY LDILRDEKSE ISDRIIEVME RYPFSSRFKE
WIPKRDILSQ KISSVLEVLS NNNNSNNNNG NNGTVPNAKT FFTPPSSITQ QVPFPSTIIP
ESTVKENSTR PYVNSHLVAN DKITATPFHS EAVVSPLQSN IRNSDIAEFD EFDIDDADFT
FNTTDPINDE SGASSDVVVI DDEEDDIENR PLNQALKASK AAVSNASLLQ SSSLDRPLLG
EMKDKNHKVL MPSLDDPMLS YPWSKEVLGC LKHKFHLKGF RKNQLEAING TLSGKDVFIL
MPTGGGKSLC YQLPAVIEGG ASRGVTLVIS PLLSLMQDQL DHLRKLNIPS LPLSGEQPAD
ERRQVISFLM AKNVLVKLLY VTPEGLASNG AITRVLKSLY ERKLLARIVI DEAHCVSHWG
HDFRPDYKQL GLLRDRYQGI PFMALTATAN EIVKKDIINT LRMENCLELK SSFNRPNLFY
EIKPKKDLYT ELYRFISNGH LHESGIIYCL SRTSCEQVAA KLRNDYGLKA WHYHAGLEKV
ERQRIQNEWQ SGSYKIIVAT IAFGMGVDKG DVRFVIHHSF PKSLEGYYQE TGRAGRDGKP
AHCIMFYSYK DHVTFQKLIM SGDGDAETKE RQRQMLRQVI QFCENKTDCR RKQVLAYFGE
NFDKVHCRKG CDICCEEATY IKQDMTEFSL QAIKLLKSIS GKATLLQLMD IFRGSKSAKI
VENGWDRLEG AGVGKLLNRG DSERLFHHLV SEGVFVEKVE ANRRGFVSAY VVPGRQTIIN
SVLAGKRRII LDVKESSSKP DTSSRSLSRS KTLPALREYQ LKSTTASVDC SIGTREVDEI
YDSQMPPVKP SLIHSRNKID LEELSGQKFM SEYEIDVMTR CLKDLKLLRS NLMAIDDSRV
SSYFTDSVLL SMAKKLPRNV KELKEIHGVS NEKAVNLGPK FLQVIQKFID EKEQNLEGTE
LDPSLQSLDT DYPIDTNALS LDHEQGFSDD SDSVYEPSSP IEEGDEEVDG QRKDILNFMN
SQSLTQTGSV PKRKSTSYTR PSKSYRHKRG STSYSRKRKY STSQKDSRKT SKSANTSFIH
PMVKQNYR