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Description:
Transcription factor BAF1 (ARS-binding factor 1) (Protein ABF1)(Bidirectionally acting factor) (SFB-B) (DNA replication enhancer-binding protein OBF1).
Molecular weight: 81750
View which proteins in this organism that is involved with DNA Repair;
classified after biological processes (using data from the GOA project):
nucleotide-excision repair, DNA damage recognition( GO:0000715 )
Important dates:
01-JAN-1990, integrated into UniProtKB/Swiss-Prot.
01-NOV-1990, sequence version 3.
07-MAR-2006, entry version 57.
Phylogenetic order:
Eukaryota Fungi Ascomycota Saccharomycotina Saccharomycetes Saccharomycetales Saccharomycetaceae Saccharomyces.
To calculate the pI (Isoelectric point - the pH where a protein has a neutral charge),
go to this page and enter the protein ID (e.g 3MG_ECOLI): http://us.expasy.org/tools/pi_tool.html
Links to references in other databases for protein BAF1_YEAST:
| Database | Pointer | Add. info#1 | Add. info#2 |
| EMBL | X16385 | CAA34421.1 | - |
| EMBL | X51654 | CAA35966.1 | - |
| EMBL | M29067 | AAA66311.1 | - |
| EMBL | M63578 | AAA34823.1 | - |
| EMBL | S93804 | AAB22002.1 | - |
| EMBL | Z28111 | CAA81951.1 | - |
| EMBL | X77511 | CAA54647.1 | - |
| PIR | S29870 | S29870. | |
| GermOnline | 139868 | -.1 | |
| TRANSFAC | T00056 | -. | |
| Ensembl | YKL112W | Saccharomyces cerevisiae.1 | |
| GenomeReviews | Y13137_GR | YKL112W.1 | |
| SGD | S000001595 | ABF1. | |
| LinkHub | P14164 | -.1 | |
| GO | GO:0000790 | C:nuclear chromatin | TAS. |
| GO | GO:0003682 | F:chromatin binding | TAS. |
| GO | GO:0003700 | F:transcription factor activity | TAS. |
| GO | GO:0030466 | P:chromatin silencing at silent mating-type c... | TAS. |
| GO | GO:0006260 | P:DNA replication | TAS. |
| GO | GO:0045941 | P:positive regulation of transcription | TAS. |
| InterPro | IPR006774 | BAF1_ABF1. | |
| Pfam | PF04684 | BAF1_ABF1 | 2. |
Keywords:
Activator; Complete proteome; Direct protein sequencing; DNA replication; DNA-binding; Metal-binding; Nuclear protein; Phosphorylation; Transcription; Transcription regulation; Zinc; Zinc-finger.
References:
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX MEDLINE=90069559; PubMed=2511628;
RA Diffley J.F.X., Stillman B.;
RT "Similarity between the transcriptional silencer binding proteins ABF1
RT and RAP1.";
RL Science 246:1034-1038(1989).
RN [2]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX MEDLINE=90152338; PubMed=2620828;
RA Rhode P.R., Sweder K.S., Oegema K.F., Campbell J.L.;
RT "The gene encoding ARS-binding factor I is essential for the viability
RT of yeast.";
RL Genes Dev. 3:1926-1939(1989).
RN [3]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 128-154 AND
RP 535-555, AND MUTAGENESIS OF HIS-57 AND CYS-71.
RX MEDLINE=90076149; PubMed=2686983;
RA Halfter H., Kavety B., Vandekerckhove J., Kiefer F., Gallwitz D.;
RT "Sequence, expression and mutational analysis of BAF1, a
RT transcriptional activator and ARS1-binding protein of the yeast
RT Saccharomyces cerevisiae.";
RL EMBO J. 8:4265-4272(1989).
RN [4]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND PHOSPHORYLATION.
RX MEDLINE=91239512; PubMed=2034654;
RA Francesconi S.C., Eisenberg S.;
RT "The multifunctional protein OBF1 is phosphorylated at serine and
RT threonine residues in Saccharomyces cerevisiae.";
RL Proc. Natl. Acad. Sci. U.S.A. 88:4089-4093(1991).
RN [5]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX MEDLINE=92221689; PubMed=1561835;
RA Jacquier A., Legrain P., Dujon B.;
RT "Sequence of a 10.7 kb segment of yeast chromosome XI identifies the
RT APN1 and the BAF1 loci and reveals one tRNA gene and several new open
RT reading frames including homologs to RAD2 and kinases.";
RL Yeast 8:121-132(1992).
RN [6]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=S288c;
RX MEDLINE=94255012; PubMed=8196765; DOI=10.1038/369371a0;
RA Dujon B., Alexandraki D., Andre B., Ansorge W., Baladron V.,
RA Ballesta J.P.G., Banrevi A., Bolle P.-A., Bolotin-Fukuhara M.,
RA Bossier P., Bou G., Boyer J., Buitrago M.J., Cheret G., Colleaux L.,
RA Daignan-Fornier B., del Rey F., Dion C., Domdey H., Duesterhoeft A.,
RA Duesterhus S., Entian K.-D., Erfle H., Esteban P.F., Feldmann H.,
RA Fernandes L., Fobo G.M., Fritz C., Fukuhara H., Gabel C., Gaillon L.,
RA Garcia-Cantalejo J.M., Garcia-Ramirez J.J., Gent M.E., Ghazvini M.,
RA Goffeau A., Gonzalez A., Grothues D., Guerreiro P., Hegemann J.H.,
RA Hewitt N., Hilger F., Hollenberg C.P., Horaitis O., Indge K.J.,
RA Jacquier A., James C.M., Jauniaux J.-C., Jimenez A., Keuchel H.,
RA Kirchrath L., Kleine K., Koetter P., Legrain P., Liebl S., Louis E.J.,
RA Maia e Silva A., Marck C., Monnier A.-L., Moestl D., Mueller S.,
RA Obermaier B., Oliver S.G., Pallier C., Pascolo S., Pfeiffer F.,
RA Philippsen P., Planta R.J., Pohl F.M., Pohl T.M., Poehlmann R.,
RA Portetelle D., Purnelle B., Puzos V., Ramezani Rad M., Rasmussen S.W.,
RA Remacha M.A., Revuelta J.L., Richard G.-F., Rieger M.,
RA Rodrigues-Pousada C., Rose M., Rupp T., Santos M.A., Schwager C.,
RA Sensen C., Skala J., Soares H., Sor F., Stegemann J., Tettelin H.,
RA Thierry A., Tzermia M., Urrestarazu L.A., van Dyck L.,
RA van Vliet-Reedijk J.C., Valens M., Vandenbol M., Vilela C.,
RA Vissers S., von Wettstein D., Voss H., Wiemann S., Xu G.,
RA Zimmermann J., Haasemann M., Becker I., Mewes H.-W.;
RT "Complete DNA sequence of yeast chromosome XI.";
RL Nature 369:371-378(1994).
RN [7]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 585-731.
RX MEDLINE=94344141; PubMed=8065362;
RA Butler A.R., White J.H., Folawiyo Y., Edlin A., Gardiner D.,
RA Stark M.J.R.;
RT "Two Saccharomyces cerevisiae genes which control sensitivity to G1
RT arrest induced by Kluyveromyces lactis toxin.";
RL Mol. Cell. Biol. 14:6306-6316(1994).
RN [8]
RP PHOSPHORYLATION SITE SER-720, AND MUTAGENESIS OF SER-720.
RX MEDLINE=95332319; PubMed=7608180; DOI=10.1074/jbc.270.27.16153;
RA Upton T., Wiltshire S., Francesconi S., Eisenberg S.;
RT "ABF1 Ser-720 is a predominant phosphorylation site for casein kinase
RT II of Saccharomyces cerevisiae.";
RL J. Biol. Chem. 270:16153-16159(1995).
RN [9]
RP PHOSPHORYLATION SITE SER-467.
RX MEDLINE=21864552; PubMed=11875433; DOI=10.1038/nbt0302-301;
RA Ficarro S.B., McCleland M.L., Stukenberg P.T., Burke D.J., Ross M.M.,
RA Shabanowitz J., Hunt D.F., White F.M.;
RT "Phosphoproteome analysis by mass spectrometry and its application to
RT Saccharomyces cerevisiae.";
RL Nat. Biotechnol. 20:301-305(2002).
RN [10]
RP LEVEL OF PROTEIN EXPRESSION.
RX MEDLINE=22923965; PubMed=14562106; DOI=10.1038/nature02046;
RA Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A.,
RA Dephoure N., O'Shea E.K., Weissman J.S.;
RT "Global analysis of protein expression in yeast.";
RL Nature 425:737-741(2003).
Feature:
CHAIN 1 731 Transcription factor BAF1.
/FTId=PRO_0000064807.
ZN_FING 49 71 CHC2-type.
MOD_RES 467 467 Phosphoserine.
MOD_RES 624 624 Phosphoserine (by PKC) (Potential).
MOD_RES 720 720 Phosphoserine (by CK2).
MUTAGEN 57 57 H->Q: Loss of DNA binding.
MUTAGEN 71 71 C->S: Loss of DNA binding.
MUTAGEN 720 720 S->A: Strongly reduces phosphorylation by
CK2.
CONFLICT 125 125 N -> K (in Ref. 3).
CONFLICT 128 128 A -> V (in Ref. 3, 5 and 6).
CONFLICT 148 148 I -> T (in Ref. 3, 5 and 6).
CONFLICT 279 280 TN -> NT (in Ref. 3, 5 and 6).
CONFLICT 690 690 N -> T (in Ref. 3, 5, 6 and 7).
Comments:
-!- FUNCTION: Trans-acting factor in the regulation of transcription
and in DNA replication. Involved in the transcription activation
of a subset of ribosomal proteins genes. Binds the ARS-elements
found in many promoters. Binds to the sequence 5'-TCN(7)ACG-3'.
-!- SUBCELLULAR LOCATION: Nucleus.
-!- PTM: Extensively phosphorylated on Ser and Thr residues.
-!- MISCELLANEOUS: Present with 4820 molecules/cell.
-!- SIMILARITY: Belongs to the BAF1 family.
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Sequence length: 731
MDKLVVNYYE YKHPIINKDL AIGAHGGKKF PTLGAWYDVI NEYEFQTRCP IILKNSHRNK
HFTFACHLKN CPFKVLLSYA GNAASSETSS PSANNNTNPP GTPDHIHHHS NNMNNEDNDN
NNGSNNKASN DSKLDFVTDD LEYHLANIHP DDTNDKVESR SNEVNGNNDD DADANNIFKQ
QGVTIKNDTE DDSINKASID RGLDDESGPT HGNDSGNHRH NEEDDVHTQM TKNYSDVVND
EDINVAIANA VANVDSQSNN KHDGKDDDAT NNNDGQDNTN NNDHNNNSNI NNNNVGSHGI
SSHSPSSIRD TSMNLDVFNS ATDDIPGPFV VTKIEPYHSH PLEDNLSLGK FILTKIPKIL
QNDLKFDQIL ESSYNNSNHT VSKFKVSHYV EESGLLDILM QRYGLTAEDF EKRLLSQIAR
RITTYKARFV LKKKKMGEYN DLQPSSSSNN NNNNDGELSG TNLRSNSIDY AKHQEISSAG
TSSNTTKNVN NNKNDSNDDN NGNNNNDASN LMESVLDKTS SHRYQPKKMP SVNKWSKPDQ
ITHSDVSMVG LDESNDGGNE NVHPTLAEVD AQEARETAQL AIDKINSYKR SIDDKNGDGH
NNSSRNVVDE NLINDMDSED AHKSKRQHLS DITLEERNED DKLPHEVAEQ LRLLSSHLKE
VENLHQNNDD DVDDVMVDVD VESQYNKNTN HHNNHHSQPH HDEEDVAGLI GKADDEEDLS
DENIQPELRG Q