Description:
Chromatin assembly factor 1 P60 subunit (CAF-1 60 kDa subunit).
Molecular weight: 51252
View which proteins in this organism that is involved with DNA Repair;
classified after biological processes (using data from the GOA project):
DNA repair( GO:0006281 )
Important dates:
01-NOV-1997, integrated into UniProtKB/Swiss-Prot.
01-NOV-1997, sequence version 1.
07-MAR-2006, entry version 43.
Phylogenetic order:
Eukaryota Fungi Ascomycota Saccharomycotina Saccharomycetes Saccharomycetales Saccharomycetaceae Saccharomyces.
To calculate the pI (Isoelectric point - the pH where a protein has a neutral charge),
go to this page and enter the protein ID (e.g 3MG_ECOLI): http://us.expasy.org/tools/pi_tool.html
Links to references in other databases for protein CAC2_YEAST:
| Database | Pointer | Add. info#1 | Add. info#2 |
| EMBL | X80835 | CAA56795.1 | - |
| PIR | S47447 | S47447. | |
| IntAct | Q04199 | -.1 | |
| GermOnline | 142636 | -.1 | |
| Ensembl | YML102W | Saccharomyces cerevisiae.1 | |
| GenomeReviews | Z71257_GR | YML102W.1 | |
| SGD | S000004570 | CAC2. | |
| BioCyc | SCER-S28-01:SCER-S28-01-004474-MONOMER | -.1 | |
| LinkHub | Q04199 | -.1 | |
| GO | GO:0005678 | C:chromatin assembly complex | IDA. |
| GO | GO:0000775 | C:chromosome, pericentric region | IDA. |
| GO | GO:0005515 | F:protein binding | IPI. |
| GO | GO:0030528 | F:transcription regulator activity | IMP. |
| GO | GO:0006342 | P:chromatin silencing | IMP. |
| GO | GO:0006281 | P:DNA repair | IMP. |
| GO | GO:0006334 | P:nucleosome assembly | IMP. |
| InterPro | IPR001680 | WD40. | |
| Pfam | PF00400 | WD40 | 5. |
| PRINTS | PR00320 | GPROTEINBRPT. | |
| ProDom | PD000018 | WD40 | 1. |
| SMART | SM00320 | WD40 | 5. |
| PROSITE | PS00678 | WD_REPEATS_1 | FALSE_NEG. |
| PROSITE | PS50082 | WD_REPEATS_2 | 2. |
| PROSITE | PS50294 | WD_REPEATS_REGION | 1. |
Keywords:
Complete proteome; Direct protein sequencing; Repeat; WD repeat.
References:
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=S288c / AB972;
RX MEDLINE=97313268; PubMed=9169872;
RA Bowman S., Churcher C.M., Badcock K., Brown D., Chillingworth T.,
RA Connor R., Dedman K., Devlin K., Gentles S., Hamlin N., Hunt S.,
RA Jagels K., Lye G., Moule S., Odell C., Pearson D., Rajandream M.A.,
RA Rice P., Skelton J., Walsh S.V., Whitehead S., Barrell B.G.;
RT "The nucleotide sequence of Saccharomyces cerevisiae chromosome
RT XIII.";
RL Nature 387:90-93(1997).
RN [2]
RP PROTEIN SEQUENCE OF 58-83 AND 459-468, AND CHARACTERIZATION.
RX MEDLINE=97182548; PubMed=9030687;
RA Kaufman P.D., Kobayashi R., Stillman B.;
RT "Ultraviolet radiation sensitivity and reduction of telomeric
RT silencing in Saccharomyces cerevisiae cells lacking chromatin assembly
RT factor-I.";
RL Genes Dev. 11:345-357(1997).
RN [3]
RP LEVEL OF PROTEIN EXPRESSION.
RX MEDLINE=22923965; PubMed=14562106; DOI=10.1038/nature02046;
RA Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A.,
RA Dephoure N., O'Shea E.K., Weissman J.S.;
RT "Global analysis of protein expression in yeast.";
RL Nature 425:737-741(2003).
Feature:
CHAIN 1 468 Chromatin assembly factor 1 P60 subunit.
/FTId=PRO_0000050894.
REPEAT 11 52 WD 1.
REPEAT 69 108 WD 2.
REPEAT 143 182 WD 3.
REPEAT 185 224 WD 4.
REPEAT 371 413 WD 5.
Comments:
-!- FUNCTION: Complex that assembles histone octamers onto replicating
DNA in vitro. CAF-1 performs the first step of the nucleosome
assembly process, bringing newly synthesized histones H3 and H4 to
replicating DNA; histones H2A/H2B can bind to this chromatin
precursor subsequent to DNA replication to complete the histone
octamer. P150 and P60 form complexes with newly synthesized
histones H3 and acetylated H4 in cell extracts (By similarity).
-!- SUBUNIT: Composed of three subunits, P50, P60 and P90.
-!- INTERACTION:
Q12495:CAC1; NbExp=1; IntAct=EBI-3920, EBI-3913;
-!- SUBCELLULAR LOCATION: Nucleus.
-!- MISCELLANEOUS: Present with 2130 molecules/cell.
-!- SIMILARITY: Belongs to the WD repeat HIR1 family.
-!- SIMILARITY: Contains 5 WD repeats.
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Sequence length: 468
MEASHLQIYW HDSQPVYSLT FQKNSANDKL FTAGGDNKVR IWKLNRDENG QNGGVRKIES
LDFLGSLTHH EQAINVIRFN SKGDVLASAG DDGQVLLWKQ EDPNTQQESV VRPFGMDAET
SEADENKEKW VVWKRLRGGS GATAAAEIYD LAWSPDNRNI VVACMDNSIR LFDVGAGMLV
CGQSDHGHYV QGVAWDPLNQ FILSQSADRS LHVYGVILSS AGVVTGLKLR SKIAKAELPC
PGDVLRTNYL FHNETLPSFF RRCSISPCGG LVVIPSGVYK VAGDEVANCV YVYTRSGILN
SAGGVKNRPA IRIPSLKKPA LMAAFSPVFY ETCQKSVLKL PYKLVFAIAT TNEVLVYDTD
VLEPLCVVGN IHYSPITDLA WSEDGSTLLI SSTDGFCSYV SIDTETQFGS RIEPPAMHAE
PLDTDESAVA AKNQREAGGI VNMLPVKKIP CNSSDSKKRR IHPTPVDL