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Description:
DNA polymerase alpha-binding protein (CTF4 protein) (Protein POB1)(Chromosome replication protein CHL15).
Molecular weight: 10429
View which proteins in this organism that is involved with DNA Repair;
classified after biological processes (using data from the GOA project):
DNA repair( GO:0006281 )
Important dates:
01-JUL-1993, integrated into UniProtKB/Swiss-Prot.
01-JUL-1993, sequence version 1.
07-MAR-2006, entry version 53.
Phylogenetic order:
Eukaryota Fungi Ascomycota Saccharomycotina Saccharomycetes Saccharomycetales Saccharomycetaceae Saccharomyces.
To calculate the pI (Isoelectric point - the pH where a protein has a neutral charge),
go to this page and enter the protein ID (e.g 3MG_ECOLI): http://us.expasy.org/tools/pi_tool.html
Links to references in other databases for protein CTF4_YEAST:
| Database | Pointer | Add. info#1 | Add. info#2 |
| EMBL | M94769 | AAA34887.1 | - |
| EMBL | S63246 | AAB27308.1 | - |
| EMBL | U40829 | AAB68276.1 | - |
| PIR | A45039 | A45039. | |
| IntAct | Q01454 | -.1 | |
| GermOnline | 144400 | -.1 | |
| Ensembl | YPR135W | Saccharomyces cerevisiae.1 | |
| GenomeReviews | U00094_GR | YPR135W.1 | |
| SGD | S000006339 | CTF4. | |
| LinkHub | Q01454 | -.1 | |
| GO | GO:0000228 | C:nuclear chromosome | IDA. |
| GO | GO:0006281 | P:DNA repair | IGI. |
| GO | GO:0006261 | P:DNA-dependent DNA replication | IMP. |
| GO | GO:0007064 | P:mitotic sister chromatid cohesion | IMP. |
| GO | GO:0001302 | P:replicative cell aging | IMP. |
| InterPro | IPR001680 | WD40. | |
| PROSITE | PS00678 | WD_REPEATS_1 | FALSE_NEG. |
| PROSITE | PS50082 | WD_REPEATS_2 | FALSE_NEG. |
| PROSITE | PS50294 | WD_REPEATS_REGION | FALSE_NEG. |
Keywords:
Complete proteome; Direct protein sequencing; DNA replication; Nuclear protein; Repeat; WD repeat.
References:
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND PROTEIN SEQUENCE OF 1-20.
RC STRAIN=7208-12;
RX MEDLINE=93078774; PubMed=1448101;
RA Miles J., Formosa T.;
RT "Evidence that POB1, a Saccharomyces cerevisiae protein that binds to
RT DNA polymerase alpha, acts in DNA metabolism in vivo.";
RL Mol. Cell. Biol. 12:5724-5735(1992).
RN [2]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX MEDLINE=93078775; PubMed=1341195;
RA Kouprina N.Y., Kroll E.S., Bannikov V.M., Bliskovsky V.V.,
RA Gizatullin R.Z., Kirillov A.V., Shestopalov B.V., Zakharyev V.M.,
RA Hieter P., Spencer F., Larionov V.;
RT "CTF4 (CHL15) mutants exhibit defective DNA metabolism in the yeast
RT Saccharomyces cerevisiae.";
RL Mol. Cell. Biol. 12:5736-5747(1992).
RN [3]
RP ERRATUM.
RA Kouprina N.Y., Kroll E.S., Bannikov V.M., Bliskovsky V.V.,
RA Gizatullin R.Z., Kirillov A.V., Shestopalov B.V., Zakharyev V.M.,
RA Hieter P., Spencer F., Larionov V.;
RL Mol. Cell. Biol. 13:7202-7202(1993).
RN [4]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX MEDLINE=93302761; PubMed=8316240;
RA Kouprina N.Y., Kroll E.S., Koryabin M.Y., Shestopalov B.V.,
RA Bliskovsky V.V., Bannikov V.M., Gizatullin R.Z., Kirillov A.V.,
RA Kravtsov V.Y., Zakharyev V.M.;
RT "CHL15 -- a new gene controlling the replication of chromosomes in
RT Saccharomycetes yeast: cloning, physical mapping, sequencing, and
RT sequence analysis.";
RL Mol. Biol. (Mosk.) 27:569-588(1993).
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=S288c / AB972;
RX MEDLINE=97313271; PubMed=9169875;
RA Bussey H., Storms R.K., Ahmed A., Albermann K., Allen E., Ansorge W.,
RA Araujo R., Aparicio A., Barrell B.G., Badcock K., Benes V.,
RA Botstein D., Bowman S., Brueckner M., Carpenter J., Cherry J.M.,
RA Chung E., Churcher C.M., Coster F., Davis K., Davis R.W.,
RA Dietrich F.S., Delius H., DiPaolo T., Dubois E., Duesterhoeft A.,
RA Duncan M., Floeth M., Fortin N., Friesen J.D., Fritz C., Goffeau A.,
RA Hall J., Hebling U., Heumann K., Hilbert H., Hillier L.W.,
RA Hunicke-Smith S., Hyman R.W., Johnston M., Kalman S., Kleine K.,
RA Komp C., Kurdi O., Lashkari D., Lew H., Lin A., Lin D., Louis E.J.,
RA Marathe R., Messenguy F., Mewes H.-W., Mirtipati S., Moestl D.,
RA Mueller-Auer S., Namath A., Nentwich U., Oefner P., Pearson D.,
RA Petel F.X., Pohl T.M., Purnelle B., Rajandream M.A., Rechmann S.,
RA Rieger M., Riles L., Roberts D., Schaefer M., Scharfe M., Scherens B.,
RA Schramm S., Schroeder M., Sdicu A.-M., Tettelin H., Urrestarazu L.A.,
RA Ushinsky S., Vierendeels F., Vissers S., Voss H., Walsh S.V.,
RA Wambutt R., Wang Y., Wedler E., Wedler H., Winnett E., Zhong W.-W.,
RA Zollner A., Vo D.H., Hani J.;
RT "The nucleotide sequence of Saccharomyces cerevisiae chromosome XVI.";
RL Nature 387:103-105(1997).
RN [6]
RP LEVEL OF PROTEIN EXPRESSION.
RX MEDLINE=22923965; PubMed=14562106; DOI=10.1038/nature02046;
RA Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A.,
RA Dephoure N., O'Shea E.K., Weissman J.S.;
RT "Global analysis of protein expression in yeast.";
RL Nature 425:737-741(2003).
Feature:
INIT_MET 0 0
CHAIN 1 926 DNA polymerase alpha-binding protein.
/FTId=PRO_0000050948.
REPEAT 9 48 WD 1.
REPEAT 133 172 WD 2.
REPEAT 226 265 WD 3.
REPEAT 272 312 WD 4.
REPEAT 698 738 WD 5.
CONFLICT 111 111 G -> E (in Ref. 2 and 4).
CONFLICT 587 587 V -> F (in Ref. 2).
CONFLICT 589 589 V -> F (in Ref. 2).
CONFLICT 600 600 N -> I (in Ref. 2).
CONFLICT 834 834 E -> K (in Ref. 2).
Comments:
-!- FUNCTION: Accessory factor for DNA replication. It plays a role in
accurately duplicating the genome in vivo.
-!- SUBCELLULAR LOCATION: Nucleus.
-!- MISCELLANEOUS: Present with 3280 molecules/cell.
-!- SIMILARITY: Contains 5 WD repeats.
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Sequence length: 926
VSVIDKLVFD FGGKTLVSLA PDNNTLCVAN KNGLTKILKT NNPEEEPETL DSSKLVSSIK
CYSNSHFLMT TMQGDALRYN IDSSQEELLA RFALPLRDCC VIHSGKMAVF GGDDLELILL
ELDDETHKKH AIKIDEQVSQ ISYNSQMNIL AVSMINGKVQ IFSLTSTIPN KVHELNDYIV
ANSYDDTHRD KILSNMMDDI DKDNDNDLSE TADPDENNVA DPEFCAANRI CTRVAWHPKG
LHFALPCADD TVKIFSIKGY SLQKTLSTNL SSTKAHFIDL QFDPLRGTYI AAVDLNNKLT
VWNWETSEIH YTREFKRKIT NIAWKIQADS KTLDLVLGTW SGSIAIVQNL AESVVSNIPD
QSVAESSTKH GLFVDSESDL ENLEGNDDIN KSDKLFSDIT QEANAEDVFT QTHDGPSGLS
EKRKYNFEDE EDFIDDDDGA GYISGKKPHN EHSYSRVHKT HSFPISLANT GKFRYMPFSP
AGTPFGFTDR RYLTMNEVGY VSTVKNSEQY SITVSFFDVG RFREYHFEDL FGYDLCFLNE
KGTLFGQSKT GQIQYRPHDS IHSNWTKIIP LQAGERITSV AATPVRVIVG TSLGYFRSFN
QFGVPFAVEK TSPIVALTAQ NYRVFSVHYS QFHGLSYSLS ELGTSSKRYY KRECPLPMSL
PNINSDMKKD ANLDYYNFNP MGIKSLFFSS YGDPCIFGSD NTLLLLSKWR SPEESKWLPI
LDSNMEIWKM SGGKETTDIH VWPLALAYDT LNCILVKGKH IWPEFPLPLP SEMEIRMPVF
VKSKLLEENK AILNKKNEIG ADTEAEEGEE DKEIQIPVSM AAEEEYLRSK VLSELLTDTL
ENDGEMYGNE NEVLAALNGA YDKALLRLFA SACSDQNVEK ALSLAHELKQ DRALTAAVKI
SERAELPSLV KKINNIREAR YEQQLK