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Description:
DNA ligase 4 (EC 6.5.1.1) (DNA ligase IV) (Polydeoxyribonucleotidesynthase [ATP] 4) (DNA ligase II).
Molecular weight: 10851
View which proteins in this organism that is involved with DNA Repair;
classified after biological processes (using data from the GOA project):
DNA repair( GO:0006281 ) double-strand break repair via nonhomologous end-joining( GO:0006303 )
Important dates:
01-NOV-1997, integrated into UniProtKB/Swiss-Prot.
01-NOV-1996, sequence version 1.
07-MAR-2006, entry version 45.
Phylogenetic order:
Eukaryota Fungi Ascomycota Saccharomycotina Saccharomycetes Saccharomycetales Saccharomycetaceae Saccharomyces.
To calculate the pI (Isoelectric point - the pH where a protein has a neutral charge),
go to this page and enter the protein ID (e.g 3MG_ECOLI): http://us.expasy.org/tools/pi_tool.html
Links to references in other databases for protein DNL4_YEAST:
| Database | Pointer | Add. info#1 | Add. info#2 |
| EMBL | U43491 | AAC49485.1 | ALT_SEQ |
| EMBL | U43491 | AAC49484.1 | ALT_SEQ |
| EMBL | Z74913 | CAA99193.1 | - |
| PIR | S66870 | S66870. | |
| PDB | 1Z56 | X-ray | C=681-944. |
| IntAct | Q08387 | -.1 | |
| GermOnline | 143593 | -.1 | |
| Ensembl | YOR005C | Saccharomyces cerevisiae.1 | |
| GenomeReviews | Y13140_GR | YOR005C.1 | |
| SGD | S000005531 | DNL4. | |
| BioCyc | SCER-S28-01:SCER-S28-01-005455-MONOMER | -.1 | |
| LinkHub | Q08387 | -.1 | |
| GO | GO:0005634 | C:nucleus | TAS. |
| GO | GO:0003910 | F:DNA ligase (ATP) activity | IDA. |
| GO | GO:0006303 | P:double-strand break repair via nonhomologou... | IDA. |
| InterPro | IPR001357 | BRCT. | |
| InterPro | IPR000977 | DNA_ligase. | |
| InterPro | IPR012309 | DNA_ligase_A_C. | |
| InterPro | IPR012310 | DNA_ligase_A_M. | |
| PANTHER | PTHR10459 | DNA_ligase.1 | 1. |
| Pfam | PF00533 | BRCT | 2. |
| Pfam | PF01068 | DNA_ligase_A_M | 1. |
| SMART | SM00292 | BRCT | 1. |
| TIGRFAMs | TIGR00574 | dnl1 | 1. |
| PROSITE | PS50172 | BRCT | 2. |
| PROSITE | PS00697 | DNA_LIGASE_A1 | 1. |
| PROSITE | PS00333 | DNA_LIGASE_A2 | 1. |
| PROSITE | PS50160 | DNA_LIGASE_A3 | 1. |
Keywords:
3D-structure; ATP-binding; Cell cycle; Cell division; Complete proteome; DNA damage; DNA recombination; DNA repair; DNA replication; Ligase; Nuclear protein; Nucleotide-binding; Repeat.
References:
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX MEDLINE=97051599; PubMed=8896276;
RX DOI=10.1002/(SICI)1097-0061(199609)12:10B<1091::AID-YEA22>3.3.CO;2-9;
RA Sterky F., Holmberg A., Pettersson B., Uhlen M.;
RT "The sequence of a 30 kb fragment on the left arm of chromosome XV
RT from Saccharomyces cerevisiae reveals 15 open reading frames, five of
RT which correspond to previously identified genes.";
RL Yeast 12:1091-1095(1996).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=S288c / FY1679;
RX MEDLINE=97313270; PubMed=9169874;
RA Dujon B., Albermann K., Aldea M., Alexandraki D., Ansorge W.,
RA Arino J., Benes V., Bohn C., Bolotin-Fukuhara M., Bordonne R.,
RA Boyer J., Camasses A., Casamayor A., Casas C., Cheret G.,
RA Cziepluch C., Daignan-Fornier B., Dang V.-D., de Haan M., Delius H.,
RA Durand P., Fairhead C., Feldmann H., Gaillon L., Galisson F.,
RA Gamo F.-J., Gancedo C., Goffeau A., Goulding S.E., Grivell L.A.,
RA Habbig B., Hand N.J., Hani J., Hattenhorst U., Hebling U.,
RA Hernando Y., Herrero E., Heumann K., Hiesel R., Hilger F., Hofmann B.,
RA Hollenberg C.P., Hughes B., Jauniaux J.-C., Kalogeropoulos A.,
RA Katsoulou C., Kordes E., Lafuente M.J., Landt O., Louis E.J.,
RA Maarse A.C., Madania A., Mannhaupt G., Marck C., Martin R.P.,
RA Mewes H.-W., Michaux G., Paces V., Parle-McDermott A.G., Pearson B.M.,
RA Perrin A., Pettersson B., Poch O., Pohl T.M., Poirey R.,
RA Portetelle D., Pujol A., Purnelle B., Ramezani Rad M., Rechmann S.,
RA Schwager C., Schweizer M., Sor F., Sterky F., Tarassov I.A.,
RA Teodoru C., Tettelin H., Thierry A., Tobiasch E., Tzermia M.,
RA Uhlen M., Unseld M., Valens M., Vandenbol M., Vetter I., Vlcek C.,
RA Voet M., Volckaert G., Voss H., Wambutt R., Wedler H., Wiemann S.,
RA Winsor B., Wolfe K.H., Zollner A., Zumstein E., Kleine K.;
RT "The nucleotide sequence of Saccharomyces cerevisiae chromosome XV.";
RL Nature 387:98-102(1997).
RN [3]
RP CHARACTERIZATION.
RX MEDLINE=97248602; PubMed=9092653; DOI=10.1093/nar/25.8.1485;
RA Ramos W., Tappe N., Talamantez J., Friedberg E.C., Tomkinson A.E.;
RT "Two distinct DNA ligase activities in mitotic extracts of the yeast
RT Saccharomyces cerevisiae.";
RL Nucleic Acids Res. 25:1485-1492(1997).
RN [4]
RP INTERACTION WITH LIF1.
RX PubMed=9670033; DOI=10.1093/emboj/17.14.4188;
RA Herrmann G., Lindahl T., Schar P.;
RT "Saccharomyces cerevisiae LIF1: a function involved in DNA double-
RT strand break repair related to mammalian XRCC4.";
RL EMBO J. 17:4188-4198(1998).
RN [5]
RP INTERACTION WITH POL4.
RX PubMed=12235149; DOI=10.1074/jbc.M206861200;
RA Tseng H.-M., Tomkinson A.E.;
RT "A physical and functional interaction between yeast Pol4 and Dnl4-
RT Lif1 links DNA synthesis and ligation in nonhomologous end joining.";
RL J. Biol. Chem. 277:45630-45637(2002).
Feature:
CHAIN 1 944 DNA ligase 4.
/FTId=PRO_0000059582.
DOMAIN 681 780 BRCT 1.
DOMAIN 836 941 BRCT 2.
ACT_SITE 282 282 N6-AMP-lysine intermediate (By
similarity).
Comments:
-!- FUNCTION: Has minor DNA joining activity. Can act on
oligo(PDT)/poly(rA) substrate.
-!- CATALYTIC ACTIVITY: ATP + (deoxyribonucleotide)(n) +
(deoxyribonucleotide)(m) = AMP + diphosphate +
(deoxyribonucleotide)(n+m).
-!- SUBUNIT: Interacts with LIF1 via its BRCT domain. Interacts with
POL4 in the DNL4-LIF1 complex.
-!- SUBCELLULAR LOCATION: Nucleus (Potential).
-!- SIMILARITY: Belongs to the ATP-dependent DNA ligase family.
-!- SIMILARITY: Contains 2 BRCT domains.
-!- CAUTION: Ref.1 sequence differs from that shown due to
frameshifts.
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Sequence length: 944
MISALDSIPE PQNFAPSPDF KWLCEELFVK IHEVQINGTA GTGKSRSFKY YEIISNFVEM
WRKTVGNNIY PALVLALPYR DRRIYNIKDY VLIRTICSYL KLPKNSATEQ RLKDWKQRVG
KGGNLSSLLV EEIAKRRAEP SSKAITIDNV NHYLDSLSGD RFASGRGFKS LVKSKPFLHC
VENMSFVELK YFFDIVLKNR VIGGQEHKLL NCWHPDAQDY LSVISDLKVV TSKLYDPKVR
LKDDDLSIKV GFAFAPQLAK KVNLSYEKIC RTLHDDFLVE EKMDGERIQV HYMNYGESIK
FFSRRGIDYT YLYGASLSSG TISQHLRFTD SVKECVLDGE MVTFDAKRRV ILPFGLVKGS
AKEALSFNSI NNVDFHPLYM VFDLLYLNGT SLTPLPLHQR KQYLNSILSP LKNIVEIVRS
SRCYGVESIK KSLEVAISLG SEGVVLKYYN SSYNVASRNN NWIKVKPEYL EEFGENLDLI
VIGRDSGKKD SFMLGLLVLD EEEYKKHQGD SSEIVDHSSQ EKHIQNSRRR VKKILSFCSI
ANGISQEEFK EIDRKTRGHW KRTSEVAPPA SILEFGSKIP AEWIDPSESI VLEIKSRSLD
NTETNMQKYA TNCTLYGGYC KRIRYDKEWT DCYTLNDLYE SRTVKSNPSY QAERSQLGLI
RKKRKRVLIS DSFHQNRKQL PISNIFAGLL FYVLSDYVTE DTGIRITRAE LEKTIVEHGG
KLIYNVILKR HSIGDVRLIS CKTTTECKAL IDRGYDILHP NWVLDCIAYK RLILIEPNYC
FNVSQKMRAV AEKRVDCLGD SFENDISETK LSSLYKSQLS LPPMGELEID SEVRRFPLFL
FSNRIAYVPR RKISTEDDII EMKIKLFGGK ITDQQSLCNL IIIPYTDPIL RKDCMNEVHE
KIKEQIKASD TIPKIARVVA PEWVDHSINE NCQVPEEDFP VVNY