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Description:
DNA ligase I, mitochondrial precursor (EC 6.5.1.1)(Polydeoxyribonucleotide synthase [ATP]).
Molecular weight: 84828
View which proteins in this organism that is involved with DNA Repair;
classified after biological processes (using data from the GOA project):
DNA ligation( GO:0006266 ) DNA repair( GO:0006281 ) base-excision repair( GO:0006284 ) nucleotide-excision repair (and GO:0045001, a synonym)( GO:0006289 )
Important dates:
13-AUG-1987, integrated into UniProtKB/Swiss-Prot.
01-NOV-1997, sequence version 2.
07-MAR-2006, entry version 70.
Phylogenetic order:
Eukaryota Fungi Ascomycota Saccharomycotina Saccharomycetes Saccharomycetales Saccharomycetaceae Saccharomyces.
To calculate the pI (Isoelectric point - the pH where a protein has a neutral charge),
go to this page and enter the protein ID (e.g 3MG_ECOLI): http://us.expasy.org/tools/pi_tool.html
Links to references in other databases for protein DNLI_YEAST:
| Database | Pointer | Add. info#1 | Add. info#2 |
| EMBL | X03246 | CAA27005.1 | - |
| EMBL | Z67750 | CAA91582.1 | - |
| EMBL | Z74212 | CAA98737.1 | - |
| EMBL | AY723764 | AAU09681.1 | - |
| EMBL | X68020 | CAA48158.1 | - |
| PIR | S61049 | LQBYPX. | |
| IntAct | P04819 | -.1 | |
| GermOnline | 140407 | -.1 | |
| Ensembl | YDL164C | Saccharomyces cerevisiae.1 | |
| GenomeReviews | Z71256_GR | YDL164C.1 | |
| SGD | S000002323 | CDC9. | |
| BioCyc | SCER-S28-01:SCER-S28-01-000917-MONOMER | -.1 | |
| LinkHub | P04819 | -.1 | |
| GO | GO:0005739 | C:mitochondrion | IDA. |
| GO | GO:0005634 | C:nucleus | IDA. |
| GO | GO:0005657 | C:replication fork | NAS. |
| GO | GO:0003910 | F:DNA ligase (ATP) activity | IDA. |
| GO | GO:0005515 | F:protein binding | IPI. |
| GO | GO:0006284 | P:base-excision repair | IMP. |
| GO | GO:0006266 | P:DNA ligation | IDA. |
| GO | GO:0006310 | P:DNA recombination | IMP. |
| GO | GO:0006273 | P:lagging strand elongation | IDA. |
| GO | GO:0006289 | P:nucleotide-excision repair | IMP. |
| InterPro | IPR000977 | DNA_ligase. | |
| InterPro | IPR012309 | DNA_ligase_A_C. | |
| InterPro | IPR012310 | DNA_ligase_A_M. | |
| InterPro | IPR012308 | DNA_ligase_A_N. | |
| PANTHER | PTHR10459 | DNA_ligase.1 | 1. |
| Pfam | PF04679 | DNA_ligase_A_C | 1. |
| Pfam | PF01068 | DNA_ligase_A_M | 1. |
| Pfam | PF04675 | DNA_ligase_A_N | 1. |
| TIGRFAMs | TIGR00574 | dnl1 | 1. |
| PROSITE | PS00697 | DNA_LIGASE_A1 | 1. |
| PROSITE | PS00333 | DNA_LIGASE_A2 | 1. |
| PROSITE | PS50160 | DNA_LIGASE_A3 | 1. |
Keywords:
Alternative initiation; ATP-binding; Cell cycle; Cell division; Complete proteome; DNA damage; DNA recombination; DNA repair; DNA replication; Ligase; Mitochondrion; Nuclear protein; Nucleotide-binding; Transit peptide.
References:
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX MEDLINE=86093646; PubMed=3909103;
RA Barker D.G., White J.H.M., Johnston L.H.;
RT "The nucleotide sequence of the DNA ligase gene (CDC9) from
RT Saccharomyces cerevisiae: a gene which is cell-cycle regulated and
RT induced in response to DNA damage.";
RL Nucleic Acids Res. 13:8323-8337(1985).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=S288c;
RX MEDLINE=97313263; PubMed=9169867;
RA Jacq C., Alt-Moerbe J., Andre B., Arnold W., Bahr A., Ballesta J.P.G.,
RA Bargues M., Baron L., Becker A., Biteau N., Bloecker H., Blugeon C.,
RA Boskovic J., Brandt P., Brueckner M., Buitrago M.J., Coster F.,
RA Delaveau T., del Rey F., Dujon B., Eide L.G., Garcia-Cantalejo J.M.,
RA Goffeau A., Gomez-Peris A., Granotier C., Hanemann V., Hankeln T.,
RA Hoheisel J.D., Jaeger W., Jimenez A., Jonniaux J.-L., Kraemer C.,
RA Kuester H., Laamanen P., Legros Y., Louis E.J., Moeller-Rieker S.,
RA Monnet A., Moro M., Mueller-Auer S., Nussbaumer B., Paricio N.,
RA Paulin L., Perea J., Perez-Alonso M., Perez-Ortin J.E., Pohl T.M.,
RA Prydz H., Purnelle B., Rasmussen S.W., Remacha M.A., Revuelta J.L.,
RA Rieger M., Salom D., Saluz H.P., Saiz J.E., Saren A.-M., Schaefer M.,
RA Scharfe M., Schmidt E.R., Schneider C., Scholler P., Schwarz S.,
RA Soler-Mira A., Urrestarazu L.A., Verhasselt P., Vissers S., Voet M.,
RA Volckaert G., Wagner G., Wambutt R., Wedler E., Wedler H., Woelfl S.,
RA Harris D.E., Bowman S., Brown D., Churcher C.M., Connor R., Dedman K.,
RA Gentles S., Hamlin N., Hunt S., Jones L., McDonald S., Murphy L.D.,
RA Niblett D., Odell C., Oliver K., Rajandream M.A., Richards C.,
RA Shore L., Walsh S.V., Barrell B.G., Dietrich F.S., Mulligan J.T.,
RA Allen E., Araujo R., Aviles E., Berno A., Carpenter J., Chen E.,
RA Cherry J.M., Chung E., Duncan M., Hunicke-Smith S., Hyman R.W.,
RA Komp C., Lashkari D., Lew H., Lin D., Mosedale D., Nakahara K.,
RA Namath A., Oefner P., Oh C., Petel F.X., Roberts D., Schramm S.,
RA Schroeder M., Shogren T., Shroff N., Winant A., Yelton M.A.,
RA Botstein D., Davis R.W., Johnston M., Andrews S., Brinkman R.,
RA Cooper J., Ding H., Du Z., Favello A., Fulton L., Gattung S.,
RA Greco T., Hallsworth K., Hawkins J., Hillier L.W., Jier M.,
RA Johnson D., Johnston L., Kirsten J., Kucaba T., Langston Y.,
RA Latreille P., Le T., Mardis E., Menezes S., Miller N., Nhan M.,
RA Pauley A., Peluso D., Rifkin L., Riles L., Taich A., Trevaskis E.,
RA Vignati D., Wilcox L., Wohldman P., Vaudin M., Wilson R.,
RA Waterston R., Albermann K., Hani J., Heumann K., Kleine K.,
RA Mewes H.-W., Zollner A., Zaccaria P.;
RT "The nucleotide sequence of Saccharomyces cerevisiae chromosome IV.";
RL Nature 387:75-78(1997).
RN [3]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=S288c;
RA Marsischky G., Rolfs A., Richardson A., Kane M., Baqui M., Taycher E.,
RA Hu Y., Vannberg F., Weger J., Kramer J., Moreira D., Kelley F.,
RA Zuo D., Raphael J., Hogle C., Jepson D., Williamson J., Camargo A.,
RA Gonzaga L., Vasconcelos A.T., Simpson A., Kolodner R., Harlow E.,
RA LaBaer J.;
RT "Creation of the YFLEX clone resource: cloning of Saccharomyces
RT cerevisiae ORFs in the Gateway recombinational cloning system.";
RL Submitted (AUG-2004) to the EMBL/GenBank/DDBJ databases.
RN [4]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 610-755.
RC STRAIN=AH22;
RX MEDLINE=93247548; PubMed=8483449;
RA Wehner E.P., Rao E., Brendel M.;
RT "Molecular structure and genetic regulation of SFA, a gene responsible
RT for resistance to formaldehyde in Saccharomyces cerevisiae, and
RT characterization of its protein product.";
RL Mol. Gen. Genet. 237:351-358(1993).
RN [5]
RP ALTERNATIVE INITIATION.
RX MEDLINE=20003237; PubMed=10531002; DOI=10.1016/S0960-9822(99)80477-1;
RA Willer M., Rainey M., Pullen T., Stirling C.J.;
RT "The yeast CDC9 gene encodes both a nuclear and a mitochondrial form
RT of DNA ligase I.";
RL Curr. Biol. 9:1085-1094(1999).
RN [6]
RP LEVEL OF PROTEIN EXPRESSION.
RX MEDLINE=22923965; PubMed=14562106; DOI=10.1038/nature02046;
RA Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A.,
RA Dephoure N., O'Shea E.K., Weissman J.S.;
RT "Global analysis of protein expression in yeast.";
RL Nature 425:737-741(2003).
Feature:
TRANSIT 1 44 Mitochondrion (Potential).
CHAIN 24 755 DNA ligase I, isoform Nuclear.
/FTId=PRO_0000007273.
INIT_MET 24 24 For isoform Nuclear.
CHAIN 45 755 DNA ligase I, isoform Mitochondrial.
/FTId=PRO_0000007274.
ACT_SITE 419 419 N6-AMP-lysine intermediate (By
similarity).
CONFLICT 69 69 D -> E (in Ref. 1).
CONFLICT 186 186 L -> V (in Ref. 1).
CONFLICT 671 671 G -> E (in Ref. 4).
CONFLICT 724 724 R -> I (in Ref. 4).
Comments:
-!- FUNCTION: This protein seals nicks in double-stranded DNA during
DNA replication, DNA recombination and DNA repair. The
mitochondrial form is required for mitochondrial DNA maintenance
but is non-essential while the nuclear form is essential for cell
viability.
-!- CATALYTIC ACTIVITY: ATP + (deoxyribonucleotide)(n) +
(deoxyribonucleotide)(m) = AMP + diphosphate +
(deoxyribonucleotide)(n+m).
-!- INTERACTION:
P15891:ABP1; NbExp=1; IntAct=EBI-5991, EBI-2036;
-!- SUBCELLULAR LOCATION: Nuclear and mitochondrial.
-!- ALTERNATIVE PRODUCTS:
Event=Alternative initiation;
Comment=2 isoforms, Mitochondrial (shown here) and Nuclear, are
produced by alternative initiation;
-!- MISCELLANEOUS: Cdc9 is included within the category of so-called
'start genes', encoding proteins which are required in early G1,
when the cell is faced with the option of initiating a further
cell cycle.
-!- MISCELLANEOUS: Present with 149 molecules/cell.
-!- SIMILARITY: Belongs to the ATP-dependent DNA ligase family.
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Sequence length: 755
MRRLLTGCLL SSARPLKSRL PLLMSSSLPS SAGKKPKQAT LARFFTSMKN KPTEGTPSPK
KSSKHMLEDR MDNVSGEEEY ATKKLKQTAV THTVAAPSSM GSNFSSIPSS APSSGVADSP
QQSQRLVGEV EDALSSNNND HYSSNIPYSE VCEVFNKIEA ISSRLEIIRI CSDFFIKIMK
QSSKNLIPTT YLFINRLGPD YEAGLELGLG ENLLMKTISE TCGKSMSQIK LKYKDIGDLG
EIAMGARNVQ PTMFKPKPLT VGEVFKNLRA IAKTQGKDSQ LKKMKLIKRM LTACKGIEAK
FLIRSLESKL RIGLAEKTVL ISLSKALLLH DENREDSPDK DVPMDVLESA QQKIRDAFCQ
VPNYEIVINS CLEHGIMNLD KYCTLRPGIP LKPMLAKPTK AINEVLDRFQ GETFTSEYKY
DGERAQVHLL NDGTMRIYSR NGENMTERYP EINITDFIQD LDTTKNLILD CEAVAWDKDQ
GKILPFQVLS TRKRKDVELN DVKVKVCLFA FDILCYNDER LINKSLKERR EYLTKVTKVV
PGEFQYATQI TTNNLDELQK FLDESVNHSC EGLMVKMLEG PESHYEPSKR SRNWLKLKKD
YLEGVGDSLD LCVLGAYYGR GKRTGTYGGF LLGCYNQDTG EFETCCKIGT GFSDEMLQLL
HDRLTPTIID GPKATFVFDS SAEPDVWFEP TTLFEVLTAD LSLSPIYKAG SATFDKGVSL
RFPRFLRIRE DKGVEDATSS DQIVELYENQ SHMQN