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Description:
Protein DOA1.
Molecular weight: 79506
View which proteins in this organism that is involved with DNA Repair;
classified after biological processes (using data from the GOA project):
double-strand break repair via nonhomologous end-joining( GO:0006303 )
Important dates:
01-JUN-1994, integrated into UniProtKB/Swiss-Prot.
01-JUN-1994, sequence version 1.
07-MAR-2006, entry version 45.
Phylogenetic order:
Eukaryota Fungi Ascomycota Saccharomycotina Saccharomycetes Saccharomycetales Saccharomycetaceae Saccharomyces.
To calculate the pI (Isoelectric point - the pH where a protein has a neutral charge),
go to this page and enter the protein ID (e.g 3MG_ECOLI): http://us.expasy.org/tools/pi_tool.html
Links to references in other databases for protein DOA1_YEAST:
| Database | Pointer | Add. info#1 | Add. info#2 |
| EMBL | U39947 | AAA82258.1 | - |
| EMBL | X75951 | CAA53560.1 | - |
| EMBL | Z28213 | CAA82058.1 | - |
| PIR | S38051 | S38051. | |
| HSSP | P16649 | 1ERJ | |
| IntAct | P36037 | -.1 | |
| GermOnline | 139967 | -.1 | |
| Ensembl | YKL213C | Saccharomyces cerevisiae.1 | |
| GenomeReviews | Y13137_GR | YKL213C.1 | |
| SGD | S000001696 | DOA1. | |
| BioCyc | SCER-S28-01:SCER-S28-01-003692-MONOMER | -.1 | |
| LinkHub | P36037 | -.1 | |
| GO | GO:0005737 | C:cytoplasm | IDA. |
| GO | GO:0005634 | C:nucleus | IDA. |
| GO | GO:0006303 | P:double-strand break repair via nonhomologou... | IMP. |
| GO | GO:0006511 | P:ubiquitin-dependent protein catabolism | IMP. |
| InterPro | IPR001680 | WD40. | |
| Pfam | PF00400 | WD40 | 6. |
| PRINTS | PR00320 | GPROTEINBRPT. | |
| ProDom | PD000018 | WD40 | 1. |
| SMART | SM00320 | WD40 | 5. |
| PROSITE | PS00678 | WD_REPEATS_1 | FALSE_NEG. |
| PROSITE | PS50082 | WD_REPEATS_2 | 4. |
| PROSITE | PS50294 | WD_REPEATS_REGION | 1. |
Keywords:
Complete proteome; Direct protein sequencing; Nuclear protein; Repeat; WD repeat.
References:
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RA Hochstrasser M., Gang G.;
RL Submitted (NOV-1995) to the EMBL/GenBank/DDBJ databases.
RN [2]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=S288c;
RX MEDLINE=95028164; PubMed=7941750;
RA Tzermia M., Horaitis O., Alexandraki D.;
RT "The complete sequencing of a 24.6 kb segment of yeast chromosome XI
RT identified the known loci URA1, SAC1 and TRP3, and revealed 6 new open
RT reading frames including homologues to the threonine dehydratases,
RT membrane transporters, hydantoinases and the phospholipase A2-
RT activating protein.";
RL Yeast 10:663-679(1994).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=S288c / FY1679;
RX MEDLINE=94255012; PubMed=8196765; DOI=10.1038/369371a0;
RA Dujon B., Alexandraki D., Andre B., Ansorge W., Baladron V.,
RA Ballesta J.P.G., Banrevi A., Bolle P.-A., Bolotin-Fukuhara M.,
RA Bossier P., Bou G., Boyer J., Buitrago M.J., Cheret G., Colleaux L.,
RA Daignan-Fornier B., del Rey F., Dion C., Domdey H., Duesterhoeft A.,
RA Duesterhus S., Entian K.-D., Erfle H., Esteban P.F., Feldmann H.,
RA Fernandes L., Fobo G.M., Fritz C., Fukuhara H., Gabel C., Gaillon L.,
RA Garcia-Cantalejo J.M., Garcia-Ramirez J.J., Gent M.E., Ghazvini M.,
RA Goffeau A., Gonzalez A., Grothues D., Guerreiro P., Hegemann J.H.,
RA Hewitt N., Hilger F., Hollenberg C.P., Horaitis O., Indge K.J.,
RA Jacquier A., James C.M., Jauniaux J.-C., Jimenez A., Keuchel H.,
RA Kirchrath L., Kleine K., Koetter P., Legrain P., Liebl S., Louis E.J.,
RA Maia e Silva A., Marck C., Monnier A.-L., Moestl D., Mueller S.,
RA Obermaier B., Oliver S.G., Pallier C., Pascolo S., Pfeiffer F.,
RA Philippsen P., Planta R.J., Pohl F.M., Pohl T.M., Poehlmann R.,
RA Portetelle D., Purnelle B., Puzos V., Ramezani Rad M., Rasmussen S.W.,
RA Remacha M.A., Revuelta J.L., Richard G.-F., Rieger M.,
RA Rodrigues-Pousada C., Rose M., Rupp T., Santos M.A., Schwager C.,
RA Sensen C., Skala J., Soares H., Sor F., Stegemann J., Tettelin H.,
RA Thierry A., Tzermia M., Urrestarazu L.A., van Dyck L.,
RA van Vliet-Reedijk J.C., Valens M., Vandenbol M., Vilela C.,
RA Vissers S., von Wettstein D., Voss H., Wiemann S., Xu G.,
RA Zimmermann J., Haasemann M., Becker I., Mewes H.-W.;
RT "Complete DNA sequence of yeast chromosome XI.";
RL Nature 369:371-378(1994).
RN [4]
RP PROTEIN SEQUENCE OF 126-144; 173-183; 215-227; 316-335; 345-361;
RP 384-420; 534-541; 598-613; 654-685 AND 700-706, AND FUNCTION.
RX PubMed=15096053; DOI=10.1021/bi035626r;
RA Russell N.S., Wilkinson K.D.;
RT "Identification of a novel 29-linked polyubiquitin binding protein,
RT Ufd3, using polyubiquitin chain analogues.";
RL Biochemistry 43:4844-4854(2004).
RN [5]
RP FUNCTION.
RA Ghislain M., Dohmen R.J., Levy F., Varshavsky A.;
RT "Cdc48p interacts with Ufd3p, a WD repeat protein required for
RT ubiquitin-mediated proteolysis in Saccharomyces cerevisiae.";
RL EMBO J. 15:4884-4899(1996).
RN [6]
RP SUBCELLULAR LOCATION.
RX MEDLINE=22923954; PubMed=14562095; DOI=10.1038/nature02026;
RA Huh W.-K., Falvo J.V., Gerke L.C., Carroll A.S., Howson R.W.,
RA Weissman J.S., O'Shea E.K.;
RT "Global analysis of protein localization in budding yeast.";
RL Nature 425:686-691(2003).
RN [7]
RP LEVEL OF PROTEIN EXPRESSION.
RX MEDLINE=22923965; PubMed=14562106; DOI=10.1038/nature02046;
RA Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A.,
RA Dephoure N., O'Shea E.K., Weissman J.S.;
RT "Global analysis of protein expression in yeast.";
RL Nature 425:737-741(2003).
Feature:
CHAIN 1 715 Protein DOA1.
/FTId=PRO_0000050958.
REPEAT 11 40 WD 1.
REPEAT 53 82 WD 2.
REPEAT 97 125 WD 3.
REPEAT 135 166 WD 4.
REPEAT 177 206 WD 5.
REPEAT 218 247 WD 6.
REPEAT 259 288 WD 7.
CONFLICT 180 180 D -> DI (in Ref. 4).
Comments:
-!- FUNCTION: Involved in ubiquitin-dependent proteolysis.
-!- SUBCELLULAR LOCATION: Nuclear, and cytoplasmic.
-!- MISCELLANEOUS: Present with 6800 molecules/cell.
-!- SIMILARITY: Belongs to the WD repeat PLAP family.
-!- SIMILARITY: Contains 7 WD repeats.
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Sequence length: 715
MGYQLSATLK GHDQDVRDVV AVDDSKVASV SRDGTVRLWS KDDQWLGTVV YTGQGFLNSV
CYDSEKELLL FGGKDTMING VPLFATSGED PLYTLIGHQG NVCSLSFQDG VVISGSWDKT
AKVWKEGSLV YNLQAHNASV WDAKVVSFSE NKFLTASADK TIKLWQNDKV IKTFSGIHND
VVRHLAVVDD GHFISCSNDG LIKLVDMHTG DVLRTYEGHE SFVYCIKLLP NGDIVSCGED
RTVRIWSKEN GSLKQVITLP AISIWSVDCM SNGDIIVGSS DNLVRIFSQE KSRWASEDEI
NELSTQVEKS TISSKTIEFD ESKLSPYEIL QSPGRKEGQI VVVKSPQGTI EAHQFSNSSW
KKVGDVVGAG ATGNDKKIEF EGKTYDYVFD VDIEDGKPPL KLPINVSDNP YTAADNFLAR
YELPMSYRDQ VVQFILKNTN GISLDQPNDN ASSSAVSPSK TSVMKVLPVK QYLIMENYNP
DTIFNGIVKI NSNEKTFDDE ILAQIGGALH DIDESWELLL SFANTIRSNW EIKTPAYDIV
RLIVKKLPYS SDIKDYIEEG LGNKNITLTM LTVRILVNCF NNENWGVKLL ESNQVYKSIF
ETIDTEFSQA SAKQSQNLAI AVSTLIFNYS ALVTKGNSDL ELLPIVADAI NTKYGPLEEY
QECEEAAYRL TVAYGNLATV EPTLRQFANS VTWLANIKRS YGNVPRFKDI FDDLS