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Description:
DNA polymerase epsilon subunit C (EC 2.7.7.7) (DNA polymerase IIsubunit C).
Molecular weight: 22665
View which proteins in this organism that is involved with DNA Repair;
classified after biological processes (using data from the GOA project):
nucleotide-excision repair (and GO:0045001, a synonym)( GO:0006289 ) mismatch repair( GO:0006298 )
Important dates:
01-AUG-1992, integrated into UniProtKB/Swiss-Prot.
01-OCT-1994, sequence version 2.
07-MAR-2006, entry version 44.
Phylogenetic order:
Eukaryota Fungi Ascomycota Saccharomycotina Saccharomycetes Saccharomycetales Saccharomycetaceae Saccharomyces.
To calculate the pI (Isoelectric point - the pH where a protein has a neutral charge),
go to this page and enter the protein ID (e.g 3MG_ECOLI): http://us.expasy.org/tools/pi_tool.html
Links to references in other databases for protein DPB3_YEAST:
| Database | Pointer | Add. info#1 | Add. info#2 |
| EMBL | X58500 | CAA41403.1 | - |
| EMBL | X76053 | CAA53641.1 | - |
| EMBL | Z36146 | CAA85242.1 | - |
| PIR | S44540 | S44540. | |
| IntAct | P27344 | -.1 | |
| GermOnline | 138821 | -.1 | |
| Ensembl | YBR278W | Saccharomyces cerevisiae.1 | |
| GenomeReviews | Y13134_GR | YBR278W.1 | |
| SGD | S000000482 | DPB3. | |
| BioCyc | SCER-S28-01:SCER-S28-01-000547-MONOMER | -.1 | |
| LinkHub | P27344 | -.1 | |
| GO | GO:0008622 | C:epsilon DNA polymerase complex | IDA. |
| GO | GO:0005657 | C:replication fork | TAS. |
| GO | GO:0003893 | F:epsilon DNA polymerase activity | TAS. |
| GO | GO:0006348 | P:chromatin silencing at telomere | IMP. |
| GO | GO:0006273 | P:lagging strand elongation | TAS. |
| GO | GO:0006272 | P:leading strand elongation | TAS. |
| GO | GO:0006298 | P:mismatch repair | TAS. |
| GO | GO:0006289 | P:nucleotide-excision repair | TAS. |
Keywords:
Complete proteome; DNA replication; DNA-directed DNA polymerase; Nuclear protein; Nucleotidyltransferase; Transferase.
References:
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=S288c / A364A;
RX MEDLINE=92020117; PubMed=1923754;
RA Araki H., Hamatake R.K., Morrison A., Johnson A.L., Johnston L.H.,
RA Sugino A.;
RT "Cloning DPB3, the gene encoding the third subunit of DNA polymerase
RT II of Saccharomyces cerevisiae.";
RL Nucleic Acids Res. 19:4867-4872(1991).
RN [2]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=S288c;
RX MEDLINE=94378722; PubMed=8091861;
RA Holmstroem K., Brandt T., Kallesoe T.;
RT "The sequence of a 32,420 bp segment located on the right arm of
RT chromosome II from Saccharomyces cerevisiae.";
RL Yeast 10:S47-S62(1994).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=S288c;
RX MEDLINE=95112788; PubMed=7813418;
RA Feldmann H., Aigle M., Aljinovic G., Andre B., Baclet M.C., Barthe C.,
RA Baur A., Becam A.-M., Biteau N., Boles E., Brandt T., Brendel M.,
RA Brueckner M., Bussereau F., Christiansen C., Contreras R., Crouzet M.,
RA Cziepluch C., Demolis N., Delaveau T., Doignon F., Domdey H.,
RA Duesterhus S., Dubois E., Dujon B., El Bakkoury M., Entian K.-D.,
RA Feuermann M., Fiers W., Fobo G.M., Fritz C., Gassenhuber J.,
RA Glansdorff N., Goffeau A., Grivell L.A., de Haan M., Hein C.,
RA Herbert C.J., Hollenberg C.P., Holmstroem K., Jacq C., Jacquet M.,
RA Jauniaux J.-C., Jonniaux J.-L., Kallesoee T., Kiesau P., Kirchrath L.,
RA Koetter P., Korol S., Liebl S., Logghe M., Lohan A.J.E., Louis E.J.,
RA Li Z.Y., Maat M.J., Mallet L., Mannhaupt G., Messenguy F., Miosga T.,
RA Molemans F., Mueller S., Nasr F., Obermaier B., Perea J., Pierard A.,
RA Piravandi E., Pohl F.M., Pohl T.M., Potier S., Proft M., Purnelle B.,
RA Ramezani Rad M., Rieger M., Rose M., Schaaff-Gerstenschlaeger I.,
RA Scherens B., Schwarzlose C., Skala J., Slonimski P.P., Smits P.H.M.,
RA Souciet J.-L., Steensma H.Y., Stucka R., Urrestarazu L.A.,
RA van der Aart Q.J.M., Van Dyck L., Vassarotti A., Vetter I.,
RA Vierendeels F., Vissers S., Wagner G., de Wergifosse P., Wolfe K.H.,
RA Zagulski M., Zimmermann F.K., Mewes H.-W., Kleine K.;
RT "Complete DNA sequence of yeast chromosome II.";
RL EMBO J. 13:5795-5809(1994).
RN [4]
RP SUBUNIT, AND SUBCELLULAR LOCATION.
RX MEDLINE=20482200; PubMed=11024162; DOI=10.1093/nar/28.20.3846;
RA Ohya T., Maki S., Kawasaki Y., Sugino A.;
RT "Structure and function of the fourth subunit (Dpb4p) of DNA
RT polymerase epsilon in Saccharomyces cerevisiae.";
RL Nucleic Acids Res. 28:3846-3852(2000).
RN [5]
RP SUBUNIT.
RX PubMed=12571237; DOI=10.1074/jbc.M211818200;
RA Chilkova O., Jonsson B.-H., Johansson E.;
RT "The quaternary structure of DNA polymerase epsilon from Saccharomyces
RT cerevisiae.";
RL J. Biol. Chem. 278:14082-14086(2003).
RN [6]
RP LEVEL OF PROTEIN EXPRESSION.
RX MEDLINE=22923965; PubMed=14562106; DOI=10.1038/nature02046;
RA Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A.,
RA Dephoure N., O'Shea E.K., Weissman J.S.;
RT "Global analysis of protein expression in yeast.";
RL Nature 425:737-741(2003).
Feature:
CHAIN 1 201 DNA polymerase epsilon subunit C.
/FTId=PRO_0000208350.
COMPBIAS 120 154 Asp/Glu-rich (acidic).
CONFLICT 196 196 T -> M (in Ref. 1).
Comments:
-!- FUNCTION: DNA polymerase II participates in chromosomal DNA
replication. DPB3 is dispensable for cell growth.
-!- CATALYTIC ACTIVITY: Deoxynucleoside triphosphate + DNA(n) =
diphosphate + DNA(n+1).
-!- SUBUNIT: Heterotetramer. Consists of four subunits: POL2, DPB2,
DPB3 and DPB4.
-!- SUBCELLULAR LOCATION: Nucleus.
-!- MISCELLANEOUS: In eukaryotes there are five DNA polymerases:
alpha, beta, gamma, delta, and epsilon which are responsible for
different reactions of DNA synthesis.
-!- MISCELLANEOUS: Present with 784 molecules/cell.
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Sequence length: 201
MSNLVKEKAP VFPISKVKKI AKCDPEYVIT SNVAISATAF AAELFVQNLV EESLVLAQLN
SKGKTSLRLS LNSIEECVEK RDNFRFLEDA IKQLKKNSAL DKKRELNMQP GRSDQEVVIE
EPELHEDDGV EEEEEEDEVS EEEEPVHNEE LLDDSKDQQN DKSTRSVASL LSRFQYKSAL
DVGEHSDSSD IEVDHTKSTD P