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Description:
DNA polymerase delta catalytic subunit (EC 2.7.7.7) (DNA polymeraseIII).
Molecular weight: 1245
View which proteins in this organism that is involved with DNA Repair;
classified after biological processes (using data from the GOA project):
base-excision repair( GO:0006284 ) nucleotide-excision repair (and GO:0045001, a synonym)( GO:0006289 ) mismatch repair( GO:0006298 ) postreplication repair( GO:0006301 )
Important dates:
01-APR-1990, integrated into UniProtKB/Swiss-Prot.
01-OCT-1996, sequence version 3.
07-MAR-2006, entry version 63.
Phylogenetic order:
Eukaryota Fungi Ascomycota Saccharomycotina Saccharomycetes Saccharomycetales Saccharomycetaceae Saccharomyces.
To calculate the pI (Isoelectric point - the pH where a protein has a neutral charge),
go to this page and enter the protein ID (e.g 3MG_ECOLI): http://us.expasy.org/tools/pi_tool.html
Links to references in other databases for protein DPOD_YEAST:
| Database | Pointer | Add. info#1 | Add. info#2 |
| EMBL | X15477 | CAA33504.1 | ALT_SEQ |
| EMBL | X61920 | CAA43922.1 | - |
| EMBL | X95644 | CAA64911.1 | - |
| EMBL | Z74150 | CAA98669.1 | - |
| PIR | S67644 | RNBYL3. | |
| HSSP | Q56366 | 1QHT | |
| GermOnline | 140344 | -.1 | |
| Ensembl | YDL102W | Saccharomyces cerevisiae.1 | |
| GenomeReviews | Z71256_GR | YDL102W.1 | |
| SGD | S000002260 | CDC2. | |
| BioCyc | SCER-S28-01:SCER-S28-01-000851-MONOMER | -.1 | |
| LinkHub | P15436 | -.1 | |
| GO | GO:0005659 | C:delta DNA polymerase complex | TAS. |
| GO | GO:0005657 | C:replication fork | IDA. |
| GO | GO:0003891 | F:delta DNA polymerase activity | TAS. |
| GO | GO:0006284 | P:base-excision repair | TAS. |
| GO | GO:0006273 | P:lagging strand elongation | TAS. |
| GO | GO:0006272 | P:leading strand elongation | TAS. |
| GO | GO:0006298 | P:mismatch repair | NAS. |
| GO | GO:0006280 | P:mutagenesis | TAS. |
| GO | GO:0006289 | P:nucleotide-excision repair | TAS. |
| GO | GO:0006301 | P:postreplication repair | TAS. |
| InterPro | IPR006172 | DNA_pol_B. | |
| InterPro | IPR006133 | DNA_pol_B_exo. | |
| InterPro | IPR006134 | DNA_pol_B_region. | |
| InterPro | IPR004578 | Pol2. | |
| InterPro | IPR012337 | RNaseH_fold. | |
| Pfam | PF00136 | DNA_pol_B | 1. |
| Pfam | PF03104 | DNA_pol_B_exo | 1. |
| PRINTS | PR00106 | DNAPOLB. | |
| SMART | SM00486 | POLBc | 1. |
| TIGRFAMs | TIGR00592 | pol2 | 1. |
| PROSITE | PS00116 | DNA_POLYMERASE_B | 1. |
Keywords:
Complete proteome; DNA replication; DNA-binding; DNA-directed DNA polymerase; Exonuclease; Hydrolase; Metal-binding; Nuclear protein; Nuclease; Nucleotidyltransferase; Transferase; Zinc; Zinc-finger.
References:
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=YAB2;
RX MEDLINE=89356661; PubMed=2670563;
RA Boulet A., Simon M., Faye G., Bauer G.A., Burgers M.J.;
RT "Structure and function of the Saccharomyces cerevisiae CDC2 gene
RT encoding the large subunit of DNA polymerase III.";
RL EMBO J. 8:1849-1854(1989).
RN [2]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND SEQUENCE REVISION.
RX MEDLINE=92158636; PubMed=1741270;
RA Morrison A., Sugino A.;
RT "Nucleotide sequence of the POL3 gene encoding DNA polymerase III
RT (delta) of Saccharomyces cerevisiae.";
RL Nucleic Acids Res. 20:375-375(1992).
RN [3]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=S288c / FY1679;
RX MEDLINE=97051597; PubMed=8896274;
RX DOI=10.1002/(SICI)1097-0061(199609)12:10B<1077::AID-YEA8>3.3.CO;2-Q;
RA Saiz J.E., Buitrago M.J., Garcia R., Revuelta J.L., del Rey F.;
RT "The sequence of a 20.3 kb DNA fragment from the left arm of
RT Saccharomyces cerevisiae chromosome IV contains the KIN28, MSS2, PHO2,
RT POL3 and DUN1 genes, and six new open reading frames.";
RL Yeast 12:1077-1084(1996).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX MEDLINE=97313263; PubMed=9169867;
RA Jacq C., Alt-Moerbe J., Andre B., Arnold W., Bahr A., Ballesta J.P.G.,
RA Bargues M., Baron L., Becker A., Biteau N., Bloecker H., Blugeon C.,
RA Boskovic J., Brandt P., Brueckner M., Buitrago M.J., Coster F.,
RA Delaveau T., del Rey F., Dujon B., Eide L.G., Garcia-Cantalejo J.M.,
RA Goffeau A., Gomez-Peris A., Granotier C., Hanemann V., Hankeln T.,
RA Hoheisel J.D., Jaeger W., Jimenez A., Jonniaux J.-L., Kraemer C.,
RA Kuester H., Laamanen P., Legros Y., Louis E.J., Moeller-Rieker S.,
RA Monnet A., Moro M., Mueller-Auer S., Nussbaumer B., Paricio N.,
RA Paulin L., Perea J., Perez-Alonso M., Perez-Ortin J.E., Pohl T.M.,
RA Prydz H., Purnelle B., Rasmussen S.W., Remacha M.A., Revuelta J.L.,
RA Rieger M., Salom D., Saluz H.P., Saiz J.E., Saren A.-M., Schaefer M.,
RA Scharfe M., Schmidt E.R., Schneider C., Scholler P., Schwarz S.,
RA Soler-Mira A., Urrestarazu L.A., Verhasselt P., Vissers S., Voet M.,
RA Volckaert G., Wagner G., Wambutt R., Wedler E., Wedler H., Woelfl S.,
RA Harris D.E., Bowman S., Brown D., Churcher C.M., Connor R., Dedman K.,
RA Gentles S., Hamlin N., Hunt S., Jones L., McDonald S., Murphy L.D.,
RA Niblett D., Odell C., Oliver K., Rajandream M.A., Richards C.,
RA Shore L., Walsh S.V., Barrell B.G., Dietrich F.S., Mulligan J.T.,
RA Allen E., Araujo R., Aviles E., Berno A., Carpenter J., Chen E.,
RA Cherry J.M., Chung E., Duncan M., Hunicke-Smith S., Hyman R.W.,
RA Komp C., Lashkari D., Lew H., Lin D., Mosedale D., Nakahara K.,
RA Namath A., Oefner P., Oh C., Petel F.X., Roberts D., Schramm S.,
RA Schroeder M., Shogren T., Shroff N., Winant A., Yelton M.A.,
RA Botstein D., Davis R.W., Johnston M., Andrews S., Brinkman R.,
RA Cooper J., Ding H., Du Z., Favello A., Fulton L., Gattung S.,
RA Greco T., Hallsworth K., Hawkins J., Hillier L.W., Jier M.,
RA Johnson D., Johnston L., Kirsten J., Kucaba T., Langston Y.,
RA Latreille P., Le T., Mardis E., Menezes S., Miller N., Nhan M.,
RA Pauley A., Peluso D., Rifkin L., Riles L., Taich A., Trevaskis E.,
RA Vignati D., Wilcox L., Wohldman P., Vaudin M., Wilson R.,
RA Waterston R., Albermann K., Hani J., Heumann K., Kleine K.,
RA Mewes H.-W., Zollner A., Zaccaria P.;
RT "The nucleotide sequence of Saccharomyces cerevisiae chromosome IV.";
RL Nature 387:75-78(1997).
RN [5]
RP EXONUCLEASE ACTIVITY.
RX MEDLINE=91293091; PubMed=1648480;
RA Simon M., Giot L., Faye G.;
RT "The 3' to 5' exonuclease activity located in the DNA polymerase delta
RT subunit of Saccharomyces cerevisiae is required for accurate
RT replication.";
RL EMBO J. 10:2165-2170(1991).
Feature:
CHAIN 1 1097 DNA polymerase delta catalytic subunit.
/FTId=PRO_0000046454.
ZN_FING 1009 1027 C4-type (Potential).
ZN_FING 1056 1074 C4-type (Potential).
CONFLICT 78 79 DV -> EL (in Ref. 1 and 2).
Comments:
-!- FUNCTION: This polymerase possess two enzymatic activities: DNA
synthesis (polymerase) and an exonucleolytic activity that
degrades single stranded DNA in the 3' to 5' direction.
-!- CATALYTIC ACTIVITY: Deoxynucleoside triphosphate + DNA(n) =
diphosphate + DNA(n+1).
-!- SUBUNIT: Heterodimer with subunits of 125 kDa and 55 kDa. The 125
kDa subunit contains the polymerase active site and most likely
the active site for the 3'-5' exonuclease activity.
-!- SUBCELLULAR LOCATION: Nucleus.
-!- MISCELLANEOUS: In eukaryotes there are five DNA polymerases:
alpha, beta, gamma, delta, and epsilon which are responsible for
different reactions of DNA synthesis.
-!- SIMILARITY: Belongs to the DNA polymerase type-B family.
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Sequence length: 1097
MSEKRSLPMV DVKIDDEDTP QLEKKIKRQS IDHGVGSEPV STIEIIPSDS FRKYNSQGFK
AKDTDLMGTQ LESTFEQDVS QMEHDMADQE EHDLSSFERK KLPTDFDPSL YDISFQQIDA
EQSVLNGIKD ENTSTVVRFF GVTSEGHSVL CNVTGFKNYL YVPAPNSSDA NDQEQINKFV
HYLNETFDHA IDSIEVVSKQ SIWGYSGDTK LPFWKIYVTY PHMVNKLRTA FERGHLSFNS
WFSNGTTTYD NIAYTLRLMV DCGIVGMSWI TLPKGKYSMI EPNNRVSSCQ LEVSINYRNL
IAHPAEGDWS HTAPLRIMSF DIECAGRIGV FPEPEYDPVI QIANVVSIAG AKKPFIRNVF
TLNTCSPITG SMIFSHATEE EMLSNWRNFI IKVDPDVIIG YNTTNFDIPY LLNRAKALKV
NDFPYFGRLK TVKQEIKESV FSSKAYGTRE TKNVNIDGRL QLDLLQFIQR EYKLRSYTLN
AVSAHFLGEQ KEDVHYSIIS DLQNGDSETR RRLAVYCLKD AYLPLRLMEK LMALVNYTEM
ARVTGVPFSY LLARGQQIKV VSQLFRKCLE IDTVIPNMQS QASDDQYEGA TVIEPIRGYY
DVPIATLDFN SLYPSIMMAH NLCYTTLCNK ATVERLNLKI DEDYVITPNG DYFVTTKRRR
GILPIILDEL ISARKRAKKD LRDEKDPFKR DVLNGRQLAL KISANSVYGF TGATVGKLPC
LAISSSVTAY GRTMILKTKT AVQEKYCIKN GYKHDAVVVY GDTDSVMVKF GTTDLKEAMD
LGTEAAKYVS TLFKHPINLE FEKAYFPYLL INKKRYAGLF WTNPDKFDKL DQKGLASVRR
DSCSLVSIVM NKVLKKILIE RNVDGALAFV RETINDILHN RVDISKLIIS KTLAPNYTNP
QPHAVLAERM KRREGVGPNV GDRVDYVIIG GNDKLYNRAE DPLFVLENNI QVDSRYYLTN
QLQNPIISIV APIIGDKQAN GMFVVKSIKI NTGSQKGGLM SFIKKVEACK SCKGPLRKGE
GPLCSNCLAR SGELYIKALY DVRDLEEKYS RLWTQCQRCA GNLHSEVLCS NKNCDIFYMR
VKVKKELQEK VEQLSKW