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Description:
N-acetyltransferase ECO1 (EC 2.3.1.-) (Establishment of cohesionprotein 1) (Chromosome transmission fidelity protein 7).
Molecular weight: 31845
View which proteins in this organism that is involved with DNA Repair;
classified after biological processes (using data from the GOA project):
DNA repair( GO:0006281 )
Important dates:
01-NOV-1995, integrated into UniProtKB/Swiss-Prot.
01-NOV-1995, sequence version 1.
07-MAR-2006, entry version 40.
Phylogenetic order:
Eukaryota Fungi Ascomycota Saccharomycotina Saccharomycetes Saccharomycetales Saccharomycetaceae Saccharomyces.
To calculate the pI (Isoelectric point - the pH where a protein has a neutral charge),
go to this page and enter the protein ID (e.g 3MG_ECOLI): http://us.expasy.org/tools/pi_tool.html
Links to references in other databases for protein ECO1_YEAST:
| Database | Pointer | Add. info#1 | Add. info#2 |
| EMBL | D50617 | BAA09266.1 | - |
| PIR | S56282 | S56282. | |
| GermOnline | 140181 | -.1 | |
| Ensembl | YFR027W | Saccharomyces cerevisiae.1 | |
| GenomeReviews | D50617_GR | YFR027W.1 | |
| SGD | S000001923 | ECO1. | |
| BioCyc | SCER-S28-01:SCER-S28-01-001968-MONOMER | -.1 | |
| LinkHub | P43605 | -.1 | |
| GO | GO:0000790 | C:nuclear chromatin | IDA. |
| GO | GO:0016407 | F:acetyltransferase activity | IDA. |
| GO | GO:0003682 | F:chromatin binding | IDA. |
| GO | GO:0005515 | F:protein binding | IPI. |
| GO | GO:0006281 | P:DNA repair | IDA. |
| GO | GO:0006260 | P:DNA replication | IMP. |
| GO | GO:0007064 | P:mitotic sister chromatid cohesion | IMP. |
| GO | GO:0045876 | P:positive regulation of sister chromatid coh... | IMP. |
| GO | GO:0006275 | P:regulation of DNA replication | IGI. |
| GO | GO:0007088 | P:regulation of mitosis | IMP. |
| GO | GO:0000084 | P:S phase of mitotic cell cycle | IDA. |
Keywords:
Acetylation; Acyltransferase; Cell cycle; Complete proteome; DNA replication; Metal-binding; Nuclear protein; Transferase; Zinc; Zinc-finger.
References:
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, AND SUBCELLULAR LOCATION.
RA Skibbens R.V., Corson L.B., Koshland D., Hieter P.;
RT "Ctf7p is essential for sister chromatid cohesion and links mitotic
RT chromosome structure to the DNA replication machinery.";
RL Genes Dev. 13:307-319(1999).
RN [2]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, AND SUBCELLULAR LOCATION.
RC STRAIN=W303;
RX MEDLINE=99145468; PubMed=9990856;
RA Toth A., Ciosk R., Uhlmann F., Galova M., Schleiffer A., Nasmyth K.;
RT "Yeast cohesin complex requires a conserved protein, Eco1p(Ctf7), to
RT establish cohesion between sister chromatids during DNA replication.";
RL Genes Dev. 13:320-333(1999).
RN [3]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=S288c / AB972;
RX MEDLINE=96287654; PubMed=8686381;
RX DOI=10.1002/(SICI)1097-0061(199602)12:2<177::AID-YEA896>3.3.CO;2-1;
RA Eki T., Naitou M., Hagiwara H., Abe M., Ozawa M., Sasanuma S.,
RA Sasanuma M., Tsuchiya Y., Shibata T., Watanabe K., Ono A.,
RA Yamazaki M., Tashiro H., Hanaoka F., Murakami Y.;
RT "Fifteen open reading frames in a 30.8 kb region of the right arm of
RT chromosome VI from Saccharomyces cerevisiae.";
RL Yeast 12:177-190(1996).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=S288c / AB972;
RX MEDLINE=95400292; PubMed=7670463;
RA Murakami Y., Naitou M., Hagiwara H., Shibata T., Ozawa M.,
RA Sasanuma S., Sasanuma M., Tsuchiya Y., Soeda E., Yokoyama K.,
RA Yamazaki M., Tashiro H., Eki T.;
RT "Analysis of the nucleotide sequence of chromosome VI from
RT Saccharomyces cerevisiae.";
RL Nat. Genet. 10:261-268(1995).
RN [5]
RP ACETYLATION, ENZYME ACTIVITY, AND MUTAGENESIS OF GLY-211;
RP 222-ARG-LYS-223; GLY-225 AND ASP-232.
RX PubMed=11864574; DOI=10.1016/S0960-9822(02)00681-4;
RA Ivanov D., Schleiffer A., Eisenhaber F., Mechtler K., Haering C.H.,
RA Nasmyth K.;
RT "Eco1 is a novel acetyltransferase that can acetylate proteins
RT involved in cohesion.";
RL Curr. Biol. 12:323-328(2002).
RN [6]
RP FUNCTION, AND INTERACTIONS WITH CHL12; RFC1; RFC2; RFC3; RFC4; RFC5
RP AND RAD24.
RX PubMed=12665596; DOI=10.1128/MCB.23.8.2999-3007.2003;
RA Kenna M.A., Skibbens R.V.;
RT "Mechanical link between cohesion establishment and DNA replication:
RT Ctf7p/Eco1p, a cohesion establishment factor, associates with three
RT different replication factor C complexes.";
RL Mol. Cell. Biol. 23:2999-3007(2003).
RN [7]
RP INTERACTION WITH CHL1.
RX PubMed=15020404; DOI=10.1534/genetics.166.1.33;
RA Skibbens R.V.;
RT "Chl1p, a DNA helicase-like protein in budding yeast, functions in
RT sister-chromatid cohesion.";
RL Genetics 166:33-42(2004).
RN [8]
RP INTERACTION WITH MPS3.
RX PubMed=15355977; DOI=10.1074/jbc.M404324200;
RA Antoniacci L.M., Kenna M.A., Uetz P., Fields S., Skibbens R.V.;
RT "The spindle pole body assembly component Mps3p/Nep98p functions in
RT sister chromatid cohesion.";
RL J. Biol. Chem. 279:49542-49550(2004).
RN [9]
RP FUNCTION, AND MUTAGENESIS OF CYS-35 AND HIS-53.
RX PubMed=15668154; DOI=10.1016/j.cub.2004.12.052;
RA Brands A., Skibbens R.V.;
RT "Ctf7p/Eco1p exhibits acetyltransferase activity--but does it
RT matter?";
RL Curr. Biol. 15:R50-R51(2005).
Feature:
CHAIN 1 281 N-acetyltransferase ECO1.
/FTId=PRO_0000074550.
ZN_FING 33 57 CCHH-type.
MOD_RES 223 223 N6-acetyllysine (by autocatalysis).
MUTAGEN 35 35 C->Y: In ctf7-109; loss of function.
MUTAGEN 53 53 H->Y: In ctf7-108; loss of function.
MUTAGEN 211 211 G->D: Abolishes acetyltransferase
activity; but not chromatid cohesion
activity.
MUTAGEN 222 223 RK->GG: Abolishes acetyltransferase
activity; but not chromatid cohesion
activity.
MUTAGEN 225 225 G->D: Abolishes acetyltransferase
activity; but not chromatid cohesion
activity.
MUTAGEN 232 232 D->G: Abolishes acetyltransferase
activity; but not chromatid cohesion
activity.
Comments:
-!- FUNCTION: Required for establishment of sister chromatid cohesion
during S phase but not for its further maintenance during G2 or M
phases or for loading the cohesin complex onto DNA. Interacts with
the three known alternate replication factor C (RFC) complexes,
suggesting that these complexes have essential but redundant
activity in cohesion establishment. In vitro, possesses
acetyltransferase activity where it can acetylate itself and
components of the cohesin complex (MCD1, IRR1 and PDS5), but is
unable to acetylate histones. The relevance of acetyltransferase
activity is however unsure, since mutations that abolish
acetyltransferase activity retain the sister chromatid cohesion
function.
-!- SUBUNIT: Binds specifically to CHL12, RFC1, RFC2, RFC3, RFC4, RFC5
and RAD24 when members of an RFC complex. Interacts with CHL1 and
MPS3.
-!- SUBCELLULAR LOCATION: Nuclear; associated with chromatin.
-!- PTM: Autoacetylates in vitro.
-!- SIMILARITY: Belongs to the acetyltransferase family. GCN5
subfamily.
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Sequence length: 281
MKARKSQRKA GSKPNLIQSK LQVNNGSKSN KIVKCDKCEM SYSSTSIEDR AIHEKYHTLQ
LHGRKWSPNW GSIVYTERNH SRTVHLSRST GTITPLNSSP LKKSSPSITH QEEKIVYVRP
DKSNGEVRAM TEIMTLVNNE LNAPHDENVI WNSTTEEKGK AFVYIRNDRA VGIIIIENLY
GGNGKTSSRG RWMVYDSRRL VQNVYPDFKI GISRIWVCRT ARKLGIATKL IDVARENIVY
GEVIPRYQVA WSQPTDSGGK LASKYNGIMH KSGKLLLPVY I