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Description:
Exodeoxyribonuclease 1 (EC 3.1.-.-) (Exodeoxyribonuclease I)(Exonuclease I) (EXO I) (Protein DHS1).
Molecular weight: 80162
View which proteins in this organism that is involved with DNA Repair;
classified after biological processes (using data from the GOA project):
DNA repair( GO:0006281 ) base-excision repair( GO:0006284 ) nucleotide-excision repair (and GO:0045001, a synonym)( GO:0006289 ) mismatch repair( GO:0006298 )
Important dates:
01-FEB-1995, integrated into UniProtKB/Swiss-Prot.
01-OCT-1996, sequence version 2.
07-MAR-2006, entry version 52.
Phylogenetic order:
Eukaryota Fungi Ascomycota Saccharomycotina Saccharomycetes Saccharomycetales Saccharomycetaceae Saccharomyces.
To calculate the pI (Isoelectric point - the pH where a protein has a neutral charge),
go to this page and enter the protein ID (e.g 3MG_ECOLI): http://us.expasy.org/tools/pi_tool.html
Links to references in other databases for protein EXO1_YEAST:
| Database | Pointer | Add. info#1 | Add. info#2 |
| EMBL | U86134 | AAB47428.1 | - |
| EMBL | X87331 | CAA60749.1 | - |
| EMBL | Z74941 | CAA99223.1 | - |
| EMBL | S69545 | AAC60570.1 | - |
| PIR | S62169 | S62169. | |
| GermOnline | 143621 | -.1 | |
| Ensembl | YOR033C | Saccharomyces cerevisiae.1 | |
| GenomeReviews | Y13140_GR | YOR033C.1 | |
| SGD | S000005559 | EXO1. | |
| BioCyc | SCER-S28-01:SCER-S28-01-005485-MONOMER | -.1 | |
| LinkHub | P39875 | -.1 | |
| GO | GO:0005634 | C:nucleus | IPI. |
| GO | GO:0008409 | F:5'-3' exonuclease activity | IDA. |
| GO | GO:0017108 | F:5'-flap endonuclease activity | IDA. |
| GO | GO:0006298 | P:mismatch repair | IMP. |
| InterPro | IPR000513 | Exo_N_I. | |
| InterPro | IPR008918 | HhH2. | |
| InterPro | IPR006086 | XPG_I. | |
| InterPro | IPR006085 | XPG_N. | |
| InterPro | IPR006084 | XPGC_Rad. | |
| Pfam | PF00867 | XPG_I | 1. |
| Pfam | PF00752 | XPG_N | 1. |
| PRINTS | PR00853 | XPGRADSUPER. | |
| SMART | SM00279 | HhH2 | 1. |
| SMART | SM00484 | XPGI | 1. |
| SMART | SM00485 | XPGN | 1. |
| PROSITE | PS00841 | XPG_1 | 1. |
| PROSITE | PS00842 | XPG_2 | 1. |
Keywords:
Complete proteome; DNA damage; DNA excision; DNA repair; DNA-binding; Excision nuclease; Exonuclease; Hydrolase; Nuclear protein; Nuclease.
References:
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RA Tishkoff D.X., Boerger A.L., Filosi N., Gaida G.M., Bertrand P.,
RA Kane M.F., Kolodner R.D.;
RT "Identification and characterization of EXO1, a gene encoding an
RT exonuclease which interacts with MSH2.";
RL Submitted (FEB-1997) to the EMBL/GenBank/DDBJ databases.
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=S288c / FY1679;
RX MEDLINE=97313270; PubMed=9169874;
RA Dujon B., Albermann K., Aldea M., Alexandraki D., Ansorge W.,
RA Arino J., Benes V., Bohn C., Bolotin-Fukuhara M., Bordonne R.,
RA Boyer J., Camasses A., Casamayor A., Casas C., Cheret G.,
RA Cziepluch C., Daignan-Fornier B., Dang V.-D., de Haan M., Delius H.,
RA Durand P., Fairhead C., Feldmann H., Gaillon L., Galisson F.,
RA Gamo F.-J., Gancedo C., Goffeau A., Goulding S.E., Grivell L.A.,
RA Habbig B., Hand N.J., Hani J., Hattenhorst U., Hebling U.,
RA Hernando Y., Herrero E., Heumann K., Hiesel R., Hilger F., Hofmann B.,
RA Hollenberg C.P., Hughes B., Jauniaux J.-C., Kalogeropoulos A.,
RA Katsoulou C., Kordes E., Lafuente M.J., Landt O., Louis E.J.,
RA Maarse A.C., Madania A., Mannhaupt G., Marck C., Martin R.P.,
RA Mewes H.-W., Michaux G., Paces V., Parle-McDermott A.G., Pearson B.M.,
RA Perrin A., Pettersson B., Poch O., Pohl T.M., Poirey R.,
RA Portetelle D., Pujol A., Purnelle B., Ramezani Rad M., Rechmann S.,
RA Schwager C., Schweizer M., Sor F., Sterky F., Tarassov I.A.,
RA Teodoru C., Tettelin H., Thierry A., Tobiasch E., Tzermia M.,
RA Uhlen M., Unseld M., Valens M., Vandenbol M., Vetter I., Vlcek C.,
RA Voet M., Volckaert G., Voss H., Wambutt R., Wedler H., Wiemann S.,
RA Winsor B., Wolfe K.H., Zollner A., Zumstein E., Kleine K.;
RT "The nucleotide sequence of Saccharomyces cerevisiae chromosome XV.";
RL Nature 387:98-102(1997).
RN [3]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 220-702.
RX MEDLINE=94169519; PubMed=7764548;
RA Lee Y.S., Shimizu J., Yoda K., Yamasaki M.;
RT "Molecular cloning of a gene, DHS1, which complements a drug-
RT hypersensitive mutation of the yeast Saccharomyces cerevisiae.";
RL Biosci. Biotechnol. Biochem. 58:391-395(1994).
RN [4]
RP FUNCTION.
RX MEDLINE=97265410; PubMed=9111347;
RA Fiorentini P., Huang K.N., Tishkoff D.X., Kolodner R.D.,
RA Symington L.S.;
RT "Exonuclease I of Saccharomyces cerevisiae functions in mitotic
RT recombination in vivo and in vitro.";
RL Mol. Cell. Biol. 17:2764-2773(1997).
RN [5]
RP FUNCTION.
RX MEDLINE=20556128; PubMed=11102356;
RA Kirkpatrick D.T., Ferguson J.R., Petes T.D., Symington L.S.;
RT "Decreased meiotic intergenic recombination and increased meiosis I
RT nondisjunction in exo1 mutants of Saccharomyces cerevisiae.";
RL Genetics 156:1549-1557(2000).
RN [6]
RP LEVEL OF PROTEIN EXPRESSION.
RX MEDLINE=22923965; PubMed=14562106; DOI=10.1038/nature02046;
RA Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A.,
RA Dephoure N., O'Shea E.K., Weissman J.S.;
RT "Global analysis of protein expression in yeast.";
RL Nature 425:737-741(2003).
RN [7]
RP FUNCTION.
RX PubMed=15629726; DOI=10.1016/j.molcel.2004.11.032;
RA Cotta-Ramusino C., Fachinetti D., Lucca C., Doksani Y., Lopes M.,
RA Sogo J., Foiani M.;
RT "Exo1 processes stalled replication forks and counteracts fork
RT reversal in checkpoint-defective cells.";
RL Mol. Cell 17:153-159(2005).
Feature:
CHAIN 1 702 Exodeoxyribonuclease 1.
/FTId=PRO_0000154045.
REGION 1 96 N-domain.
REGION 114 247 I-domain.
COMPBIAS 501 520 Asp/Glu-rich (acidic).
COMPBIAS 537 553 Asp/Glu-rich (acidic).
COMPBIAS 618 625 Asp-rich (acidic).
Comments:
-!- FUNCTION: 5'->3' double-stranded DNA exonuclease involved in
mismatch repair and eventually also in mitotic recombination
between direct repeats. Also has a minor role in the correction of
large DNA mismatches that occur in the heteroduplex DNA during
meiotic recombination at the HIS4 locus.
-!- COFACTOR: Magnesium or manganese.
-!- ENZYME REGULATION: Inactivated by calcium and zinc ions.
-!- SUBUNIT: Interacts with mismatch repair protein MSH2.
-!- SUBCELLULAR LOCATION: Nucleus (Potential).
-!- MISCELLANEOUS: Present with 672 molecules/cell.
-!- SIMILARITY: Belongs to the XPG/RAD2 endonuclease family. EXO1
subfamily.
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Sequence length: 702
MGIQGLLPQL KPIQNPVSLR RYEGEVLAID GYAWLHRAAC SCAYELAMGK PTDKYLQFFI
KRFSLLKTFK VEPYLVFDGD AIPVKKSTES KRRDKRKENK AIAERLWACG EKKNAMDYFQ
KCVDITPEMA KCIICYCKLN GIRYIVAPFE ADSQMVYLEQ KNIVQGIISE DSDLLVFGCR
RLITKLNDYG ECLEICRDNF IKLPKKFPLG SLTNEEIITM VCLSGCDYTN GIPKVGLITA
MKLVRRFNTI ERIILSIQRE GKLMIPDTYI NEYEAAVLAF QFQRVFCPIR KKIVSLNEIP
LYLKDTESKR KRLYACIGFV IHRETQKKQI VHFDDDIDHH LHLKIAQGDL NPYDFHQPLA
NREHKLQLAS KSNIEFGKTN TTNSEAKVKP IESFFQKMTK LDHNPKVANN IHSLRQAEDK
LTMAIKRRKL SNANVVQETL KDTRSKFFNK PSMTVVENFK EKGDSIQDFK EDTNSQSLEE
PVSESQLSTQ IPSSFITTNL EDDDNLSEEV SEVVSDIEED RKNSEGKTIG NEIYNTDDDG
DGDTSEDYSE TAESRVPTSS TTSFPGSSQR SISGCTKVLQ KFRYSSSFSG VNANRQPLFP
RHVNQKSRGM VYVNQNRDDD CDDNDGKNQI TQRPSLRKSL IGARSQRIVI DMKSVDERKS
FNSSPILHEE SKKRDIETTK SSQARPAVRS ISLLSQFVYK GK