Description:
Ligase-interacting factor 1.
Molecular weight: 48259
View which proteins in this organism that is involved with DNA Repair;
classified after biological processes (using data from the GOA project):
DNA repair( GO:0006281 ) double-strand break repair via nonhomologous end-joining( GO:0006303 )
Important dates:
01-OCT-1996, integrated into UniProtKB/Swiss-Prot.
01-OCT-1996, sequence version 1.
07-MAR-2006, entry version 33.
Phylogenetic order:
Eukaryota Fungi Ascomycota Saccharomycotina Saccharomycetes Saccharomycetales Saccharomycetaceae Saccharomyces.
To calculate the pI (Isoelectric point - the pH where a protein has a neutral charge),
go to this page and enter the protein ID (e.g 3MG_ECOLI): http://us.expasy.org/tools/pi_tool.html
Links to references in other databases for protein LIF1_YEAST:
| Database | Pointer | Add. info#1 | Add. info#2 |
| EMBL | Z72612 | CAA96796.1 | - |
| PIR | S64097 | S64097. | |
| PDB | 1Z56 | X-ray | A/B=1-246. |
| IntAct | P53150 | -.1 | |
| GermOnline | 141138 | -.1 | |
| Ensembl | YGL090W | Saccharomyces cerevisiae.1 | |
| GenomeReviews | Y13135_GR | YGL090W.1 | |
| SGD | S000003058 | LIF1. | |
| BioCyc | SCER-S28-01:SCER-S28-01-002090-MONOMER | -.1 | |
| LinkHub | P53150 | -.1 | |
| GO | GO:0005634 | C:nucleus | TAS. |
| GO | GO:0005198 | F:structural molecule activity | TAS. |
| GO | GO:0006303 | P:double-strand break repair via nonhomologou... | IDA. |
Keywords:
3D-structure; Complete proteome; DNA damage; DNA repair; Nuclear protein.
References:
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, AND INTERACTION WITH
RP DNL4.
RX PubMed=9670033; DOI=10.1093/emboj/17.14.4188;
RA Herrmann G., Lindahl T., Schar P.;
RT "Saccharomyces cerevisiae LIF1: a function involved in DNA double-
RT strand break repair related to mammalian XRCC4.";
RL EMBO J. 17:4188-4198(1998).
RN [2]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=S288c;
RX MEDLINE=97435481; PubMed=9290212;
RX DOI=10.1002/(SICI)1097-0061(19970915)13:11<1077::AID-YEA152>3.3.CO;2-P;
RA Rieger M., Brueckner M., Schaefer M., Mueller-Auer S.;
RT "Sequence analysis of 203 kilobases from Saccharomyces cerevisiae
RT chromosome VII.";
RL Yeast 13:1077-1090(1997).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=S288c / FY1679;
RX MEDLINE=97313265; PubMed=9169869;
RA Tettelin H., Agostoni-Carbone M.L., Albermann K., Albers M.,
RA Arroyo J., Backes U., Barreiros T., Bertani I., Bjourson A.J.,
RA Brueckner M., Bruschi C.V., Carignani G., Castagnoli L., Cerdan E.,
RA Clemente M.L., Coblenz A., Coglievina M., Coissac E., Defoor E.,
RA Del Bino S., Delius H., Delneri D., de Wergifosse P., Dujon B.,
RA Durand P., Entian K.-D., Eraso P., Escribano V., Fabiani L.,
RA Fartmann B., Feroli F., Feuermann M., Frontali L., Garcia-Gonzalez M.,
RA Garcia-Saez M.I., Goffeau A., Guerreiro P., Hani J., Hansen M.,
RA Hebling U., Hernandez K., Heumann K., Hilger F., Hofmann B.,
RA Indge K.J., James C.M., Klima R., Koetter P., Kramer B., Kramer W.,
RA Lauquin G., Leuther H., Louis E.J., Maillier E., Marconi A.,
RA Martegani E., Mazon M.J., Mazzoni C., McReynolds A.D.K.,
RA Melchioretto P., Mewes H.-W., Minenkova O., Mueller-Auer S.,
RA Nawrocki A., Netter P., Neu R., Nombela C., Oliver S.G., Panzeri L.,
RA Paoluzi S., Plevani P., Portetelle D., Portillo F., Potier S.,
RA Purnelle B., Rieger M., Riles L., Rinaldi T., Robben J.,
RA Rodrigues-Pousada C., Rodriguez-Belmonte E., Rodriguez-Torres A.M.,
RA Rose M., Ruzzi M., Saliola M., Sanchez-Perez M., Schaefer B.,
RA Schaefer M., Scharfe M., Schmidheini T., Schreer A., Skala J.,
RA Souciet J.-L., Steensma H.Y., Talla E., Thierry A., Vandenbol M.,
RA van der Aart Q.J.M., Van Dyck L., Vanoni M., Verhasselt P., Voet M.,
RA Volckaert G., Wambutt R., Watson M.D., Weber N., Wedler E., Wedler H.,
RA Wipfli P., Wolf K., Wright L.F., Zaccaria P., Zimmermann M.,
RA Zollner A., Kleine K.;
RT "The nucleotide sequence of Saccharomyces cerevisiae chromosome VII.";
RL Nature 387:81-84(1997).
RN [4]
RP INTERACTION OF THE DNL4-LIF1 COMPLEX WITH POL4.
RX PubMed=12235149; DOI=10.1074/jbc.M206861200;
RA Tseng H.-M., Tomkinson A.E.;
RT "A physical and functional interaction between yeast Pol4 and Dnl4-
RT Lif1 links DNA synthesis and ligation in nonhomologous end joining.";
RL J. Biol. Chem. 277:45630-45637(2002).
RN [5]
RP SUBCELLULAR LOCATION.
RX MEDLINE=22923954; PubMed=14562095; DOI=10.1038/nature02026;
RA Huh W.-K., Falvo J.V., Gerke L.C., Carroll A.S., Howson R.W.,
RA Weissman J.S., O'Shea E.K.;
RT "Global analysis of protein localization in budding yeast.";
RL Nature 425:686-691(2003).
RN [6]
RP LEVEL OF PROTEIN EXPRESSION.
RX MEDLINE=22923965; PubMed=14562106; DOI=10.1038/nature02046;
RA Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A.,
RA Dephoure N., O'Shea E.K., Weissman J.S.;
RT "Global analysis of protein expression in yeast.";
RL Nature 425:737-741(2003).
Feature:
CHAIN 1 421 Ligase-interacting factor 1.
/FTId=PRO_0000084419.
Comments:
-!- FUNCTION: Stabilizes DNL4. Involved in nonhomologous repair of DNA
double-strand breaks.
-!- SUBUNIT: Interacts with the BRCT domain of DNL4. The DNL4-LIF1
complex interacts with POL4.
-!- SUBCELLULAR LOCATION: Nuclear and cytoplasmic.
-!- MISCELLANEOUS: Present with 876 molecules/cell.
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Sequence length: 421
MSQLTEFISC IPVVNEEQNE EDERGLCKIQ IEDGAMLETL DENSLSGLRI EKMLVSEGTG
IFSKSSFGIN DLRIFTGENI DEESKKYVWY ELLKMLTGHK VYIASLDEKV VFTKWTCRMQ
DDEVWKVVME LESSAIIRKI AELTLHPVKK GEIDLFEMAD KLYKDICCVN DSYRNIKESD
SSNRNRVEQL ARERELLDKL LETRDERTRA MMVTLLNEKK KKIRELHEIL RQNNIKLSDD
DVLDSALINT EVQKPISELN SPGKRMKRRK TVVEPQNLQK KLKDTSRRRA NRKISNQSVI
KMEDDDFDDF QFFGLSKRPI ITAKDKLSEK YDDITSFGDD TQSISFESDS SSDVQKHLVS
LEDNGIQISA GRSDEDYGDI SGSESETDAS AGEKKSSNHS EQSGNDREPC LQTESETDIE
T