Description:
DNA-3-methyladenine glycosylase (EC 3.2.2.21) (3-methyladenine DNAglycosidase) (3MEA DNA glycosylase).
Molecular weight: 34333
View which proteins in this organism that is involved with DNA Repair;
classified after biological processes (using data from the GOA project):
DNA repair( GO:0006281 ) base-excision repair( GO:0006284 ) DNA dealkylation( GO:0006307 )
Important dates:
01-AUG-1991, integrated into UniProtKB/Swiss-Prot.
01-AUG-1991, sequence version 1.
07-MAR-2006, entry version 51.
Phylogenetic order:
Eukaryota Fungi Ascomycota Saccharomycotina Saccharomycetes Saccharomycetales Saccharomycetaceae Saccharomyces.
To calculate the pI (Isoelectric point - the pH where a protein has a neutral charge),
go to this page and enter the protein ID (e.g 3MG_ECOLI): http://us.expasy.org/tools/pi_tool.html
Links to references in other databases for protein MAG_YEAST:
| Database | Pointer | Add. info#1 | Add. info#2 |
| EMBL | X56662 | CAA39989.1 | - |
| EMBL | X57781 | CAA40927.1 | - |
| EMBL | U18917 | AAB64669.1 | - |
| EMBL | AY692938 | AAT92957.1 | - |
| PIR | S12498 | S12498. | |
| IntAct | P22134 | -.1 | |
| GermOnline | 139220 | -.1 | |
| Ensembl | YER142C | Saccharomyces cerevisiae.1 | |
| GenomeReviews | U00092_GR | YER142C.1 | |
| SGD | S000000944 | MAG1. | |
| BioCyc | SCER-S28-01:SCER-S28-01-001816-MONOMER | -.1 | |
| LinkHub | P22134 | -.1 | |
| GO | GO:0005634 | C:nucleus | IC. |
| GO | GO:0003905 | F:alkylbase DNA N-glycosylase activity | IDA. |
| GO | GO:0006307 | P:DNA dealkylation | IEP. |
| InterPro | IPR000035 | AlbDNA_glycsylse. | |
| InterPro | IPR003265 | Endo_3c. | |
| Pfam | PF00730 | HhH-GPD | 1. |
| SMART | SM00478 | ENDO3c | 1. |
| PROSITE | PS00516 | ALKYLBASE_DNA_GLYCOS | 1. |
Keywords:
Complete proteome; Direct protein sequencing; DNA damage; DNA repair; Hydrolase; Nuclear protein.
References:
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX MEDLINE=91092283; PubMed=2265619;
RA Berdal K.G., Bjoeraas M., Bjelland S., Seeberg E.C.;
RT "Cloning and expression in Escherichia coli of a gene for an alkylbase
RT DNA glycosylase from Saccharomyces cerevisiae; a homologue to the
RT bacterial alkA gene.";
RL EMBO J. 9:4563-4568(1990).
RN [2]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX MEDLINE=91092284; PubMed=2265620;
RA Chen J., Derfler B., Samson L.;
RT "Saccharomyces cerevisiae 3-methyladenine DNA glycosylase has homology
RT to the AlkA glycosylase of E. coli and is induced in response to DNA
RT alkylation damage.";
RL EMBO J. 9:4569-4575(1990).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=S288c / AB972;
RX MEDLINE=97313264; PubMed=9169868;
RA Dietrich F.S., Mulligan J.T., Hennessy K.M., Yelton M.A., Allen E.,
RA Araujo R., Aviles E., Berno A., Brennan T., Carpenter J., Chen E.,
RA Cherry J.M., Chung E., Duncan M., Guzman E., Hartzell G.,
RA Hunicke-Smith S., Hyman R.W., Kayser A., Komp C., Lashkari D., Lew H.,
RA Lin D., Mosedale D., Nakahara K., Namath A., Norgren R., Oefner P.,
RA Oh C., Petel F.X., Roberts D., Sehl P., Schramm S., Shogren T.,
RA Smith V., Taylor P., Wei Y., Botstein D., Davis R.W.;
RT "The nucleotide sequence of Saccharomyces cerevisiae chromosome V.";
RL Nature 387:78-81(1997).
RN [4]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=S288c;
RA Marsischky G., Rolfs A., Richardson A., Kane M., Baqui M., Taycher E.,
RA Hu Y., Vannberg F., Weger J., Kramer J., Moreira D., Kelley F.,
RA Zuo D., Raphael J., Hogle C., Jepson D., Williamson J., Camargo A.,
RA Gonzaga L., Vasconcelos A.T., Simpson A., Kolodner R., Harlow E.,
RA LaBaer J.;
RT "Creation of the YFLEX clone resource: cloning of Saccharomyces
RT cerevisiae ORFs in the Gateway recombinational cloning system.";
RL Submitted (JUL-2004) to the EMBL/GenBank/DDBJ databases.
RN [5]
RP PROTEIN SEQUENCE OF 1-11.
RA Bjoras M., Klungland A., Johansen R.F., Seeberg E.;
RT "Purification and properties of the alkylation repair DNA glycosylase
RT encoded the MAG gene from Saccharomyces cerevisiae.";
RL Biochemistry 34:4577-4582(1995).
RN [6]
RP SUBCELLULAR LOCATION.
RX MEDLINE=21393706; PubMed=11502169; DOI=10.1021/bi010277r;
RA Grandier-Vazeille X., Bathany K., Chaignepain S., Camougrand N.,
RA Manon S., Schmitter J.-M.;
RT "Yeast mitochondrial dehydrogenases are associated in a supramolecular
RT complex.";
RL Biochemistry 40:9758-9769(2001).
Feature:
CHAIN 1 296 DNA-3-methyladenine glycosylase.
/FTId=PRO_0000194883.
Comments:
-!- FUNCTION: Hydrolysis of the deoxyribose N-glycosidic bond to
excise 3-methyladenine or 7-methyladenine from the damaged DNA
polymer formed by alkylation lesions.
-!- CATALYTIC ACTIVITY: Hydrolysis of alkylated DNA, releasing 3-
methyladenine, 3-methylguanine, 7-methylguanine and 7-
methyladenine.
-!- SUBCELLULAR LOCATION: Nucleus.
-!- INDUCTION: By DNA damage.
-!- SIMILARITY: Belongs to the alkylbase DNA glycosidase alkA family.
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Sequence length: 296
MKLKREYDEL IKADAVKEIA KELGSRPLEV ALPEKYIARH EEKFNMACEH ILEKDPSLFP
ILKNNEFTLY LKETQVPNTL EDYFIRLAST ILSQQISGQA AESIKARVVS LYGGAFPDYK
ILFEDFKDPA KCAEIAKCGL SKRKMIYLES LAVYFTEKYK DIEKLFGQKD NDEEVIESLV
TNVKGIGPWS AKMFLISGLK RMDVFAPEDL GIARGFSKYL SDKPELEKEL MRERKVVKKS
KIKHKKYNWK IYDDDIMEKC SETFSPYRSV FMFILWRLAS TNTDAMMKAE ENFVKS