Description:
Proliferating cell nuclear antigen (PCNA).
Molecular weight: 28916
View which proteins in this organism that is involved with DNA Repair;
classified after biological processes (using data from the GOA project):
base-excision repair( GO:0006284 ) nucleotide-excision repair (and GO:0045001, a synonym)( GO:0006289 ) mismatch repair( GO:0006298 ) postreplication repair( GO:0006301 )
Important dates:
01-APR-1990, integrated into UniProtKB/Swiss-Prot.
01-APR-1990, sequence version 1.
07-MAR-2006, entry version 60.
Phylogenetic order:
Eukaryota Fungi Ascomycota Saccharomycotina Saccharomycetes Saccharomycetales Saccharomycetaceae Saccharomyces.
To calculate the pI (Isoelectric point - the pH where a protein has a neutral charge),
go to this page and enter the protein ID (e.g 3MG_ECOLI): http://us.expasy.org/tools/pi_tool.html
Links to references in other databases for protein PCNA_YEAST:
| Database | Pointer | Add. info#1 | Add. info#2 |
| EMBL | X16676 | CAA34664.1 | - |
| EMBL | X78993 | CAA55594.1 | - |
| EMBL | Z35957 | CAA85038.1 | - |
| EMBL | AY557715 | AAS56041.1 | - |
| PIR | S22851 | WMBYET. | |
| PDB | 1PLQ | X-ray | @=1-258. |
| PDB | 1PLR | X-ray | @=1-258. |
| PDB | 1SXJ | X-ray | F/G/H=1-258. |
| IntAct | P15873 | -.1 | |
| GermOnline | 138631 | -.1 | |
| Ensembl | YBR088C | Saccharomyces cerevisiae.1 | |
| GenomeReviews | Y13134_GR | YBR088C.1 | |
| SGD | S000000292 | POL30. | |
| BioCyc | SCER-S28-01:SCER-S28-01-000351-MONOMER | -.1 | |
| LinkHub | P15873 | -.1 | |
| GO | GO:0005634 | C:nucleus | IDA. |
| GO | GO:0005657 | C:replication fork | TAS. |
| GO | GO:0030337 | F:DNA polymerase processivity factor activity | TAS. |
| GO | GO:0005515 | F:protein binding | IPI. |
| GO | GO:0006284 | P:base-excision repair | TAS. |
| GO | GO:0006273 | P:lagging strand elongation | TAS. |
| GO | GO:0006272 | P:leading strand elongation | TAS. |
| GO | GO:0006298 | P:mismatch repair | TAS. |
| GO | GO:0006280 | P:mutagenesis | TAS. |
| GO | GO:0006289 | P:nucleotide-excision repair | TAS. |
| GO | GO:0006301 | P:postreplication repair | TAS. |
| InterPro | IPR000730 | Pr_cel_nuc_antig. | |
| PANTHER | PTHR11352 | Pr_cel_nuc_antig.1 | 1. |
| Pfam | PF02747 | PCNA_C | 1. |
| Pfam | PF00705 | PCNA_N | 1. |
| PIRSF | PIRSF002090 | PCNA | 1. |
| PRINTS | PR00339 | PCNACYCLIN. | |
| ProDom | PD002673 | Pr_cel_nuc_antig | 1. |
| TIGRFAMs | TIGR00590 | pcna | 1. |
| PROSITE | PS01251 | PCNA_1 | 1. |
| PROSITE | PS00293 | PCNA_2 | 1. |
Keywords:
3D-structure; Complete proteome; Direct protein sequencing; DNA replication; DNA-binding; Nuclear protein.
References:
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND PROTEIN SEQUENCE OF 1-41 AND
RP 231-240.
RX MEDLINE=90221805; PubMed=1970160;
RA Bauer G.A., Burgess P.M.J.;
RT "Molecular cloning, structure and expression of the yeast
RT proliferating cell nuclear antigen gene.";
RL Nucleic Acids Res. 18:261-265(1990).
RN [2]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=S288c;
RX MEDLINE=95208357; PubMed=7900426;
RA Mannhaupt G., Stucka R., Ehnle S., Vetter I., Feldmann H.;
RT "Analysis of a 70 kb region on the right arm of yeast chromosome II.";
RL Yeast 10:1363-1381(1994).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=S288c;
RX MEDLINE=95112788; PubMed=7813418;
RA Feldmann H., Aigle M., Aljinovic G., Andre B., Baclet M.C., Barthe C.,
RA Baur A., Becam A.-M., Biteau N., Boles E., Brandt T., Brendel M.,
RA Brueckner M., Bussereau F., Christiansen C., Contreras R., Crouzet M.,
RA Cziepluch C., Demolis N., Delaveau T., Doignon F., Domdey H.,
RA Duesterhus S., Dubois E., Dujon B., El Bakkoury M., Entian K.-D.,
RA Feuermann M., Fiers W., Fobo G.M., Fritz C., Gassenhuber J.,
RA Glansdorff N., Goffeau A., Grivell L.A., de Haan M., Hein C.,
RA Herbert C.J., Hollenberg C.P., Holmstroem K., Jacq C., Jacquet M.,
RA Jauniaux J.-C., Jonniaux J.-L., Kallesoee T., Kiesau P., Kirchrath L.,
RA Koetter P., Korol S., Liebl S., Logghe M., Lohan A.J.E., Louis E.J.,
RA Li Z.Y., Maat M.J., Mallet L., Mannhaupt G., Messenguy F., Miosga T.,
RA Molemans F., Mueller S., Nasr F., Obermaier B., Perea J., Pierard A.,
RA Piravandi E., Pohl F.M., Pohl T.M., Potier S., Proft M., Purnelle B.,
RA Ramezani Rad M., Rieger M., Rose M., Schaaff-Gerstenschlaeger I.,
RA Scherens B., Schwarzlose C., Skala J., Slonimski P.P., Smits P.H.M.,
RA Souciet J.-L., Steensma H.Y., Stucka R., Urrestarazu L.A.,
RA van der Aart Q.J.M., Van Dyck L., Vassarotti A., Vetter I.,
RA Vierendeels F., Vissers S., Wagner G., de Wergifosse P., Wolfe K.H.,
RA Zagulski M., Zimmermann F.K., Mewes H.-W., Kleine K.;
RT "Complete DNA sequence of yeast chromosome II.";
RL EMBO J. 13:5795-5809(1994).
RN [4]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=S288c;
RA Marsischky G., Rolfs A., Richardson A., Kane M., Baqui M., Taycher E.,
RA Hu Y., Vannberg F., Weger J., Kramer J., Moreira D., Kelley F.,
RA Zuo D., Raphael J., Hogle C., Jepson D., Williamson J., Camargo A.,
RA Gonzaga L., Vasconcelos A.T., Simpson A., Kolodner R., Harlow E.,
RA LaBaer J.;
RT "Creation of the YFLEX clone resource: cloning of Saccharomyces
RT cerevisiae ORFs in the Gateway recombinational cloning system.";
RL Submitted (FEB-2004) to the EMBL/GenBank/DDBJ databases.
RN [5]
RP X-RAY CRYSTALLOGRAPHY (2.3 ANGSTROMS).
RX MEDLINE=95094298; PubMed=8001157; DOI=10.1016/0092-8674(94)90014-0;
RA Krishna T.S., Kong X.P., Gary S., Burgers P.M., Kuriyan J.;
RT "Crystal structure of the eukaryotic DNA polymerase processivity
RT factor PCNA.";
RL Cell 79:1233-1243(1994).
Feature:
CHAIN 1 258 Proliferating cell nuclear antigen.
/FTId=PRO_0000149176.
DNA_BIND 61 80 Potential.
STRAND 2 8
HELIX 9 19
TURN 20 22
STRAND 24 31
TURN 32 33
STRAND 34 40
TURN 42 43
STRAND 44 53
HELIX 54 56
STRAND 57 60
STRAND 63 64
STRAND 66 71
HELIX 72 78
TURN 79 80
STRAND 82 82
TURN 83 85
STRAND 86 92
STRAND 94 95
STRAND 97 104
STRAND 106 109
STRAND 111 117
STRAND 119 119
STRAND 131 131
STRAND 134 140
HELIX 141 152
TURN 153 154
STRAND 156 163
TURN 164 165
STRAND 166 172
STRAND 174 175
STRAND 177 182
STRAND 185 185
TURN 188 189
HELIX 191 193
STRAND 195 201
STRAND 203 208
HELIX 209 220
TURN 221 221
STRAND 223 229
STRAND 231 233
STRAND 235 241
TURN 242 243
STRAND 244 250
STRAND 254 256
Comments:
-!- FUNCTION: This protein is an auxiliary protein of DNA polymerase
delta and is involved in the control of eukaryotic DNA replication
by increasing the polymerase's processibility during elongation of
the leading strand.
-!- SUBUNIT: Homotrimer.
-!- INTERACTION:
P26793:RAD27; NbExp=1; IntAct=EBI-12993, EBI-14693;
-!- SUBCELLULAR LOCATION: Nucleus.
-!- SIMILARITY: Belongs to the PCNA family.
-----------------------------------------------------------------------
Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms
Distributed under the Creative Commons Attribution-NoDerivs License
-----------------------------------------------------------------------
Sequence length: 258
MLEAKFEEAS LFKRIIDGFK DCVQLVNFQC KEDGIIAQAV DDSRVLLVSL EIGVEAFQEY
RCDHPVTLGM DLTSLSKILR CGNNTDTLTL IADNTPDSII LLFEDTKKDR IAEYSLKLMD
IDADFLKIEE LQYDSTLSLP SSEFSKIVRD LSQLSDSINI MITKETIKFV ADGDIGSGSV
IIKPFVDMEH PETSIKLEMD QPVDLTFGAK YLLDIIKGSS LSDRVGIRLS SEAPALFQFD
LKSGFLQFFL APKFNDEE