Protein data for UBIQ_YEAST:

Description:
Ubiquitin.

Molecular weight: 8557 M

View which proteins in this organism that is involved with DNA Repair;
classified after biological processes (using data from the GOA project):
DNA repair( GO:0006281 )


Important dates:
13-AUG-1987, integrated into UniProtKB/Swiss-Prot.
13-AUG-1987, sequence version 1.
07-FEB-2006, entry version 30.

Phylogenetic order:
Eukaryota Fungi Ascomycota Saccharomycotina Saccharomycetes Saccharomycetales Saccharomycetaceae Saccharomyces.

To calculate the pI (Isoelectric point - the pH where a protein has a neutral charge),
go to this page and enter the protein ID (e.g 3MG_ECOLI): http://us.expasy.org/tools/pi_tool.html

Links to references in other databases for protein UBIQ_YEAST:

DatabasePointerAdd. info#1Add. info#2
EMBLX01473CAA25704.1ALT_TERM
EMBLX01474CAA25706.1ALT_SEQ
EMBLX05728CAA29195.1ALT_TERM
EMBLX05729CAA29196.1ALT_TERM
EMBLX05730CAA29197.1ALT_TERM
EMBLX05731CAA29198.1ALT_TERM
EMBLX73541CAA51949.1ALT_TERM
EMBLZ28319CAA82173.1ALT_TERM
EMBLZ38059CAA86130.1ALT_TERM
EMBLU17246AAB67466.1ALT_TERM
EMBLZ73144CAA97489.1ALT_TERM
PIRA29456A29456.
PIRC29456UQBYR7.
PDB1OTRNMRB=1-76.
PDB1Q0WNMRB=1-76.
PDB1WR1NMRA=1-76.
IntActP61864-.1
GermOnline139683-.1
GermOnline140073-.1
GermOnline142034-.1
GermOnline142229-.1
SWISS-2DPAGEP61864YEAST.
SGDS000001410RPL40A.
SGDS000001802RPL40B.
SGDS000004157RPS31.
SGDS000003962UBI4.
LinkHubP61864-.1
GOGO:0005515F:protein bindingIPI.
InterProIPR000626Ubiquitin.
PfamPF00240ubiquitin1.
PRINTSPR00348UBIQUITIN.
SMARTSM00213UBQ1.
PROSITEPS00299UBIQUITIN_11.
PROSITEPS50053UBIQUITIN_21.

General information about the databases mentioned above

Keywords:
3D-structure; Complete proteome; DNA damage; DNA repair; Nuclear protein; Polyprotein; Ubl conjugation.

References:
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX MEDLINE=85061630; PubMed=6095120;
RA Oezkaynak E., Finley D., Varshavsky A.;
RT "The yeast ubiquitin gene: head-to-tail repeats encoding a
RT polyubiquitin precursor protein.";
RL Nature 312:663-666(1984).
RN [2]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA] (UBI1; UBI2; UBI3 AND UBI4).
RX MEDLINE=87275838; PubMed=3038523;
RA Oezkaynak E., Finley D., Solomon M.J., Varshavsky A.;
RT "The yeast ubiquitin genes: a family of natural gene fusions.";
RL EMBO J. 6:1429-1439(1987).
RN [3]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA] (UBI2).
RX MEDLINE=94205265; PubMed=8154186;
RA Bou G., Esteban P.F., Baladron V., Gonzalez G.A., Cantalejo J.G.,
RA Remacha M.A., Jimenez A., del Rey F., Ballesta J.P.G., Revuelta J.L.;
RT "The complete sequence of a 15,820 bp segment of Saccharomyces
RT cerevisiae chromosome XI contains the UBI2 and MPL1 genes and three
RT new open reading frames.";
RL Yeast 9:1349-1354(1993).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA] (UBI2).
RC STRAIN=S288c / FY1679;
RX MEDLINE=94255012; PubMed=8196765; DOI=10.1038/369371a0;
RA Dujon B., Alexandraki D., Andre B., Ansorge W., Baladron V.,
RA Ballesta J.P.G., Banrevi A., Bolle P.-A., Bolotin-Fukuhara M.,
RA Bossier P., Bou G., Boyer J., Buitrago M.J., Cheret G., Colleaux L.,
RA Daignan-Fornier B., del Rey F., Dion C., Domdey H., Duesterhoeft A.,
RA Duesterhus S., Entian K.-D., Erfle H., Esteban P.F., Feldmann H.,
RA Fernandes L., Fobo G.M., Fritz C., Fukuhara H., Gabel C., Gaillon L.,
RA Garcia-Cantalejo J.M., Garcia-Ramirez J.J., Gent M.E., Ghazvini M.,
RA Goffeau A., Gonzalez A., Grothues D., Guerreiro P., Hegemann J.H.,
RA Hewitt N., Hilger F., Hollenberg C.P., Horaitis O., Indge K.J.,
RA Jacquier A., James C.M., Jauniaux J.-C., Jimenez A., Keuchel H.,
RA Kirchrath L., Kleine K., Koetter P., Legrain P., Liebl S., Louis E.J.,
RA Maia e Silva A., Marck C., Monnier A.-L., Moestl D., Mueller S.,
RA Obermaier B., Oliver S.G., Pallier C., Pascolo S., Pfeiffer F.,
RA Philippsen P., Planta R.J., Pohl F.M., Pohl T.M., Poehlmann R.,
RA Portetelle D., Purnelle B., Puzos V., Ramezani Rad M., Rasmussen S.W.,
RA Remacha M.A., Revuelta J.L., Richard G.-F., Rieger M.,
RA Rodrigues-Pousada C., Rose M., Rupp T., Santos M.A., Schwager C.,
RA Sensen C., Skala J., Soares H., Sor F., Stegemann J., Tettelin H.,
RA Thierry A., Tzermia M., Urrestarazu L.A., van Dyck L.,
RA van Vliet-Reedijk J.C., Valens M., Vandenbol M., Vilela C.,
RA Vissers S., von Wettstein D., Voss H., Wiemann S., Xu G.,
RA Zimmermann J., Haasemann M., Becker I., Mewes H.-W.;
RT "Complete DNA sequence of yeast chromosome XI.";
RL Nature 369:371-378(1994).
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA] (UBI1).
RC STRAIN=S288c / AB972;
RX MEDLINE=97313266; PubMed=9169870;
RA Churcher C.M., Bowman S., Badcock K., Bankier A.T., Brown D.,
RA Chillingworth T., Connor R., Devlin K., Gentles S., Hamlin N.,
RA Harris D.E., Horsnell T., Hunt S., Jagels K., Jones M., Lye G.,
RA Moule S., Odell C., Pearson D., Rajandream M.A., Rice P., Rowley N.,
RA Skelton J., Smith V., Walsh S.V., Whitehead S., Barrell B.G.;
RT "The nucleotide sequence of Saccharomyces cerevisiae chromosome IX.";
RL Nature 387:84-87(1997).
RN [6]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA] (UBI3 AND UBI4).
RC STRAIN=S288c / AB972;
RX MEDLINE=97313267; PubMed=9169871;
RA Johnston M., Hillier L.W., Riles L., Albermann K., Andre B.,
RA Ansorge W., Benes V., Brueckner M., Delius H., Dubois E.,
RA Duesterhoeft A., Entian K.-D., Floeth M., Goffeau A., Hebling U.,
RA Heumann K., Heuss-Neitzel D., Hilbert H., Hilger F., Kleine K.,
RA Koetter P., Louis E.J., Messenguy F., Mewes H.-W., Miosga T.,
RA Moestl D., Mueller-Auer S., Nentwich U., Obermaier B., Piravandi E.,
RA Pohl T.M., Portetelle D., Purnelle B., Rechmann S., Rieger M.,
RA Rinke M., Rose M., Scharfe M., Scherens B., Scholler P., Schwager C.,
RA Schwarz S., Underwood A.P., Urrestarazu L.A., Vandenbol M.,
RA Verhasselt P., Vierendeels F., Voet M., Volckaert G., Voss H.,
RA Wambutt R., Wedler E., Wedler H., Zimmermann F.K., Zollner A.,
RA Hani J., Hoheisel J.D.;
RT "The nucleotide sequence of Saccharomyces cerevisiae chromosome XII.";
RL Nature 387:87-90(1997).
RN [7]
RP MUTAGENESIS OF LYS-29; LYS-48 AND LYS-63.
RX MEDLINE=95340540; PubMed=7615550; DOI=10.1074/jbc.270.29.17442;
RA Johnson E.S., Ma P.C.M., Ota I.M., Varshavsky A.;
RT "A proteolytic pathway that recognizes ubiquitin as a degradation
RT signal.";
RL J. Biol. Chem. 270:17442-17456(1995).
RN [8]
RP MUTAGENESIS OF LYSINE RESIDUES.
RX MEDLINE=95166210; PubMed=7862120;
RA Spence J., Sadis S., Haas A.L., Finley D.;
RT "A ubiquitin mutant with specific defects in DNA repair and
RT multiubiquitination.";
RL Mol. Cell. Biol. 15:1265-1273(1995).
RN [9]
RP X-RAY CRYSTALLOGRAPHY (2.25 ANGSTROMS) IN COMPLEX WITH YUH1.
RX PubMed=10406793; DOI=10.1093/emboj/18.14.3877;
RA Johnston S.C., Riddle S.M., Cohen R.E., Hill C.P.;
RT "Structural basis for the specificity of ubiquitin C-terminal
RT hydrolases.";
RL EMBO J. 18:3877-3887(1999).
RN [10]
RP X-RAY CRYSTALLOGRAPHY (1.7 ANGSTROMS) IN COMPLEX WITH VPS9.
RX PubMed=12628920; DOI=10.1093/emboj/cdg140;
RA Shih S.C., Prag G., Francis S.A., Sutanto M.A., Hurley J.H., Hicke L.;
RT "A ubiquitin-binding motif required for intramolecular
RT monoubiquitylation, the CUE domain.";
RL EMBO J. 22:1273-1281(2003).
RN [11]
RP STRUCTURE BY NMR IN COMPLEX WITH UBC1.
RX PubMed=11591345; DOI=10.1016/S0969-2126(01)00657-8;
RA Hamilton K.S., Ellison M.J., Barber K.R., Williams R.S., Huzil J.T.,
RA McKenna S., Ptak C., Glover M., Shaw G.S.;
RT "Structure of a conjugating enzyme-ubiquitin thiolester intermediate
RT reveals a novel role for the ubiquitin tail.";
RL Structure 9:897-904(2001).
RN [12]
RP STRUCTURE BY NMR OF COMPLEX WITH CUE2 N-TERMINAL DOMAIN.
RX PubMed=12787503; DOI=10.1016/S0092-8674(03)00362-3;
RA Kang R.S., Daniels C.M., Francis S.A., Shih S.C., Salerno W.J.,
RA Hicke L., Radhakrishnan I.;
RT "Solution structure of a CUE-ubiquitin complex reveals a conserved
RT mode of ubiquitin binding.";
RL Cell 113:621-630(2003).

Feature:
CHAIN 1 76 Ubiquitin.
/FTId=PRO_0000114861.
CROSSLNK 29 29 Glycyl lysine isopeptide (Lys-Gly)
(interchain with G-Cter in
multiubiquitin).
CROSSLNK 48 48 Glycyl lysine isopeptide (Lys-Gly)
(interchain with G-Cter in
multiubiquitin).
CROSSLNK 63 63 Glycyl lysine isopeptide (Lys-Gly)
(interchain with G-Cter in
multiubiquitin).
CROSSLNK 76 76 Glycyl lysine isopeptide (Gly-Lys)
(interchain with K-? in acceptor
proteins).
MUTAGEN 29 29 K->R: Deficiency in ubiquitin-protein
conjugate formation.
MUTAGEN 48 48 K->R: Deficiency in ubiquitin-protein
conjugate formation.
MUTAGEN 63 63 K->R: Deficiency in ubiquitin-protein
conjugate formation. Loss of DNA repair
function.
STRAND 2 10
STRAND 12 16
TURN 19 20
STRAND 21 21
HELIX 23 34
TURN 38 40
STRAND 41 45
TURN 46 47
STRAND 48 49
STRAND 52 53
HELIX 56 59
TURN 60 60
STRAND 63 64
STRAND 66 71
STRAND 73 74

Comments:
-!- FUNCTION: Involved in the ATP-dependent selective degradation of
cellular proteins, the maintenance of chromatin structure, the
regulation of gene expression, the stress response, and ribosome
biogenesis and DNA repair.
-!- INTERACTION:
P25369:LSB5; NbExp=1; IntAct=EBI-19797, EBI-10218;
Q06651:PIB1; NbExp=1; IntAct=EBI-19797, EBI-35947;
-!- SUBCELLULAR LOCATION: Nuclear and cytoplasmic.
-!- MISCELLANEOUS: UBI1 and UBI2 are synthesized as a polyprotein with
one copy of ubiquitin fused to ribosomal protein L40. UBI3 is a
polyprotein with one copy of ubiquitin fused to ribosomal protein
S37. UBI4 is a polyprotein containing 5 exact head to tail repeats
of ubiquitin, there is a final amino-acid (Asn) after the last
repeat.
-!- SIMILARITY: Belongs to the ubiquitin family.
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Sequence length: 76

   MQIFVKTLTG KTITLEVESS DTIDNVKSKI QDKEGIPPDQ QRLIFAGKQL EDGRTLSDYN
     IQKESTLHLV LRLRGG